Article: Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer

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TitleAnalysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer
AuthorsLi, D6
Zhao, Y6
Liu, C4
Chen, X6
Qi, Y5
Jiang, Y5 6
Zou, C6
Zhang, X6
Liu, S6
Wang, X1
Zhao, D6
Sun, Q1
Zeng, Z3
Dress, A3
Lin, MC6
Kung, HF6 7
Rui, H5
Liu, LZ5
Mao, F1
Jiang, BH2 5
Lai, L6
Issue Date2011
CitationClinical Cancer Research, 2011, v. 17 n. 7, p. 1722-1730 [How to Cite?]
DOI: http://dx.doi.org/10.1158/1078-0432.CCR-10-1800
AbstractPurpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer. Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues. Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer. Conclusions: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets. ©2011 AACR.
ISSN1078-0432
2011 Impact Factor: 7.742
2011 SCImago Journal Rankings: 1.066
DOIhttp://dx.doi.org/10.1158/1078-0432.CCR-10-1800
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLi, D
dc.contributor.authorZhao, Y
dc.contributor.authorLiu, C
dc.contributor.authorChen, X
dc.contributor.authorQi, Y
dc.contributor.authorJiang, Y
dc.contributor.authorZou, C
dc.contributor.authorZhang, X
dc.contributor.authorLiu, S
dc.contributor.authorWang, X
dc.contributor.authorZhao, D
dc.contributor.authorSun, Q
dc.contributor.authorZeng, Z
dc.contributor.authorDress, A
dc.contributor.authorLin, MC
dc.contributor.authorKung, HF
dc.contributor.authorRui, H
dc.contributor.authorLiu, LZ
dc.contributor.authorMao, F
dc.contributor.authorJiang, BH
dc.contributor.authorLai, L
dc.date.accessioned2012-10-08T03:19:56Z
dc.date.available2012-10-08T03:19:56Z
dc.date.issued2011
dc.description.abstractPurpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer. Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues. Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer. Conclusions: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets. ©2011 AACR.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationClinical Cancer Research, 2011, v. 17 n. 7, p. 1722-1730 [How to Cite?]
DOI: http://dx.doi.org/10.1158/1078-0432.CCR-10-1800
dc.identifier.doihttp://dx.doi.org/10.1158/1078-0432.CCR-10-1800
dc.identifier.epage1730
dc.identifier.issn1078-0432
2011 Impact Factor: 7.742
2011 SCImago Journal Rankings: 1.066
dc.identifier.issue7
dc.identifier.pmid21350001
dc.identifier.scopuseid_2-s2.0-79953330808
dc.identifier.spage1722
dc.identifier.urihttp://hdl.handle.net/10722/168518
dc.identifier.volume17
dc.languageeng
dc.publisher.placeUnited States
dc.relation.ispartofClinical Cancer Research
dc.relation.referencesReferences in Scopus
dc.subject.mesh3' Untranslated Regions
dc.subject.meshBase Sequence
dc.subject.meshBreast Neoplasms - Genetics - Metabolism - Pathology
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Movement
dc.subject.meshCell Proliferation
dc.subject.meshCpg Islands
dc.subject.meshCyclin D1 - Metabolism
dc.subject.meshDna Methylation
dc.subject.meshDown-Regulation
dc.subject.meshFemale
dc.subject.meshGene Silencing
dc.subject.meshGenes, Reporter
dc.subject.meshHumans
dc.subject.meshLuciferases, Renilla - Biosynthesis - Genetics
dc.subject.meshMicrornas - Genetics - Metabolism
dc.subject.meshNeoplasm Invasiveness
dc.subject.meshProto-Oncogene Proteins C-Raf - Metabolism
dc.subject.meshRestriction Mapping
dc.titleAnalysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer
dc.typeArticle
Author Affiliations
  1. Peking Union Medical College
  2. Nanjing Medical University
  3. Chinese Academy of Sciences
  4. Fudan University Shanghai Medical College
  5. Thomas Jefferson University
  6. East China Normal University
  7. Chinese University of Hong Kong