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Article: Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer
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TitleAnalysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer
 
AuthorsLi, D6
Zhao, Y6
Liu, C4
Chen, X6
Qi, Y5
Jiang, Y6 5
Zou, C6
Zhang, X6
Liu, S6
Wang, X1
Zhao, D6
Sun, Q1
Zeng, Z3
Dress, A3
Lin, MC6
Kung, HF6 7
Rui, H5
Liu, LZ5
Mao, F1
Jiang, BH2 5
Lai, L6
 
Issue Date2011
 
CitationClinical Cancer Research, 2011, v. 17 n. 7, p. 1722-1730 [How to Cite?]
DOI: http://dx.doi.org/10.1158/1078-0432.CCR-10-1800
 
AbstractPurpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer. Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues. Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer. Conclusions: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets. ©2011 AACR.
 
ISSN1078-0432
2013 Impact Factor: 8.193
2013 SCImago Journal Rankings: 5.150
 
DOIhttp://dx.doi.org/10.1158/1078-0432.CCR-10-1800
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, D
 
dc.contributor.authorZhao, Y
 
dc.contributor.authorLiu, C
 
dc.contributor.authorChen, X
 
dc.contributor.authorQi, Y
 
dc.contributor.authorJiang, Y
 
dc.contributor.authorZou, C
 
dc.contributor.authorZhang, X
 
dc.contributor.authorLiu, S
 
dc.contributor.authorWang, X
 
dc.contributor.authorZhao, D
 
dc.contributor.authorSun, Q
 
dc.contributor.authorZeng, Z
 
dc.contributor.authorDress, A
 
dc.contributor.authorLin, MC
 
dc.contributor.authorKung, HF
 
dc.contributor.authorRui, H
 
dc.contributor.authorLiu, LZ
 
dc.contributor.authorMao, F
 
dc.contributor.authorJiang, BH
 
dc.contributor.authorLai, L
 
dc.date.accessioned2012-10-08T03:19:56Z
 
dc.date.available2012-10-08T03:19:56Z
 
dc.date.issued2011
 
dc.description.abstractPurpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer. Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues. Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer. Conclusions: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets. ©2011 AACR.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical Cancer Research, 2011, v. 17 n. 7, p. 1722-1730 [How to Cite?]
DOI: http://dx.doi.org/10.1158/1078-0432.CCR-10-1800
 
dc.identifier.doihttp://dx.doi.org/10.1158/1078-0432.CCR-10-1800
 
dc.identifier.epage1730
 
dc.identifier.issn1078-0432
2013 Impact Factor: 8.193
2013 SCImago Journal Rankings: 5.150
 
dc.identifier.issue7
 
dc.identifier.pmid21350001
 
dc.identifier.scopuseid_2-s2.0-79953330808
 
dc.identifier.spage1722
 
dc.identifier.urihttp://hdl.handle.net/10722/168518
 
dc.identifier.volume17
 
dc.languageeng
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical Cancer Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.mesh3' Untranslated Regions
 
dc.subject.meshBase Sequence
 
dc.subject.meshBreast Neoplasms - Genetics - Metabolism - Pathology
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshCell Movement
 
dc.subject.meshCell Proliferation
 
dc.subject.meshCpg Islands
 
dc.subject.meshCyclin D1 - Metabolism
 
dc.subject.meshDna Methylation
 
dc.subject.meshDown-Regulation
 
dc.subject.meshFemale
 
dc.subject.meshGene Silencing
 
dc.subject.meshGenes, Reporter
 
dc.subject.meshHumans
 
dc.subject.meshLuciferases, Renilla - Biosynthesis - Genetics
 
dc.subject.meshMicrornas - Genetics - Metabolism
 
dc.subject.meshNeoplasm Invasiveness
 
dc.subject.meshProto-Oncogene Proteins C-Raf - Metabolism
 
dc.subject.meshRestriction Mapping
 
dc.titleAnalysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer
 
dc.typeArticle
 
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<description.abstract>Purpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer. Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues. Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer. Conclusions: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets. &#169;2011 AACR.</description.abstract>
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Author Affiliations
  1. Peking Union Medical College
  2. Nanjing Medical University
  3. Chinese Academy of Sciences
  4. Fudan University Shanghai Medical College
  5. Thomas Jefferson University
  6. East China Normal University
  7. Chinese University of Hong Kong