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Article: Microfluidic chip-capillary electrophoresis for two orders extension of adjustable upper working range for profiling of inorganic and organic anions in urine

TitleMicrofluidic chip-capillary electrophoresis for two orders extension of adjustable upper working range for profiling of inorganic and organic anions in urine
Authors
Issue Date2010
Citation
Electrophoresis, 2010, v. 31 n. 18, p. 3044-3052 How to Cite?
AbstractTo meet the need for onsite monitoring of urine anions, a microfluidic chip-capillary electrophoresis device was designed, fabricated and tested to extend the upper CE working range for an enhancement up to 500 fold (100 fold for sample dilution and 5 folds for CE injection) in order to analyze highly variable anionic metabolites in urine samples. Capillaries were embedded between two PMMA plates with laser-fabricated microchannel patterns to produce the microfluidic chip-capillary electrophoresis to perform standard/sample dilution and CE injection with adjustable dilution ratios. A circular ferrofluid valve was incorporated on-chip to perform cleanup and conditioning, mixing and dilution, injection and CE separation. Under optimized conditions, a complete assay for four samples can be achieved within an hour for 15 anions commonly found in urines. Satisfactory working ranges (0.005-500 mM) and low detection limits (0.5-6.5 μM based on S/N = 2) are obtained with satisfactory repeatability (RSD, = 55) 0.52-0.87% and 4.1-6.5% for migration time and peak area, respectively. The working ranges with two orders adjustable upper extension are adequate to cover all analytes concentrations commonly found in human urine samples. The device fabricated shows sufficiently large experimentally verifiable enhancement factor to meet the application requirements. Its reliability was established by more than 94% recoveries of spiked standards and agreeable results from parallel method comparison with conventional ion chromatography method. The extension of the upper CE working range enables flexible onsite dilution on demand, a quick turn-around of results, and a low-cost device suitable for bedside monitoring of patients under critical conditions for metabolic disorders. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/168474
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, WPen_US
dc.contributor.authorLau, KMen_US
dc.contributor.authorFung, YSen_US
dc.date.accessioned2012-10-08T03:19:25Z-
dc.date.available2012-10-08T03:19:25Z-
dc.date.issued2010en_US
dc.identifier.citationElectrophoresis, 2010, v. 31 n. 18, p. 3044-3052en_US
dc.identifier.issn0173-0835en_US
dc.identifier.urihttp://hdl.handle.net/10722/168474-
dc.description.abstractTo meet the need for onsite monitoring of urine anions, a microfluidic chip-capillary electrophoresis device was designed, fabricated and tested to extend the upper CE working range for an enhancement up to 500 fold (100 fold for sample dilution and 5 folds for CE injection) in order to analyze highly variable anionic metabolites in urine samples. Capillaries were embedded between two PMMA plates with laser-fabricated microchannel patterns to produce the microfluidic chip-capillary electrophoresis to perform standard/sample dilution and CE injection with adjustable dilution ratios. A circular ferrofluid valve was incorporated on-chip to perform cleanup and conditioning, mixing and dilution, injection and CE separation. Under optimized conditions, a complete assay for four samples can be achieved within an hour for 15 anions commonly found in urines. Satisfactory working ranges (0.005-500 mM) and low detection limits (0.5-6.5 μM based on S/N = 2) are obtained with satisfactory repeatability (RSD, = 55) 0.52-0.87% and 4.1-6.5% for migration time and peak area, respectively. The working ranges with two orders adjustable upper extension are adequate to cover all analytes concentrations commonly found in human urine samples. The device fabricated shows sufficiently large experimentally verifiable enhancement factor to meet the application requirements. Its reliability was established by more than 94% recoveries of spiked standards and agreeable results from parallel method comparison with conventional ion chromatography method. The extension of the upper CE working range enables flexible onsite dilution on demand, a quick turn-around of results, and a low-cost device suitable for bedside monitoring of patients under critical conditions for metabolic disorders. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.en_US
dc.languageengen_US
dc.relation.ispartofElectrophoresisen_US
dc.subject.meshAmino Acids - Chemistry - Urineen_US
dc.subject.meshAnions - Chemistry - Urineen_US
dc.subject.meshBiological Markers - Chemistry - Urineen_US
dc.subject.meshCarboxylic Acids - Chemistry - Urineen_US
dc.subject.meshElectrophoresis, Microchip - Instrumentation - Methodsen_US
dc.subject.meshEquipment Designen_US
dc.subject.meshHumansen_US
dc.subject.meshSensitivity And Specificityen_US
dc.titleMicrofluidic chip-capillary electrophoresis for two orders extension of adjustable upper working range for profiling of inorganic and organic anions in urineen_US
dc.typeArticleen_US
dc.identifier.emailFung, YS:ysfung@hku.hken_US
dc.identifier.authorityFung, YS=rp00697en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/elps.201000297en_US
dc.identifier.pmid20872610-
dc.identifier.scopuseid_2-s2.0-77956898378en_US
dc.identifier.hkuros181793-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956898378&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume31en_US
dc.identifier.issue18en_US
dc.identifier.spage3044en_US
dc.identifier.epage3052en_US
dc.identifier.isiWOS:000283389900005-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridGuo, WP=37025980200en_US
dc.identifier.scopusauthoridLau, KM=7401559826en_US
dc.identifier.scopusauthoridFung, YS=13309754700en_US

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