File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Time-resolved ICP-MS measurement for single-cell analysis and on-line cytometry

TitleTime-resolved ICP-MS measurement for single-cell analysis and on-line cytometry
Authors
Issue Date2010
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/jaas
Citation
Journal Of Analytical Atomic Spectrometry, 2010, v. 25 n. 7, p. 1114-1122 How to Cite?
AbstractA method of simultaneous cell counting and determination of constituent metals in single cells using time-resolved inductively coupled plasma-mass spectrometry (ICP-MS) is reported. A unicellular alga, Chlorella vulgaris, was used as a model in this study. Algae suspensions of number density of 10 6/mL were converted into fine aerosols using conventional solution nebulization for sample introduction into the ICP. The metals in each cell are converted into a plume of gaseous analyte ions in the ICP. Each metal in the plume produces a current spike at the detector of the mass spectrometer. ICP-MS intensity spikes of major elements, such as Mg of 108 atoms/cell, and trace elements, such as Mn and Cu of 5 × 106 atoms/cell, were detected readily. The number density of the cells in an algae suspension can be determined by counting the number of the ICP-MS intensity spikes of the major element (Mg) over a fixed period of time. The uncertainty in cell counting was approximately 2% RSD, similar to that of hemacytometry. The ICP-MS spike intensity of an element is related to its quantity in the algal cell. Quantitative determination of the metal contents of the algal cells using metal oxide particles for calibration is feasible. Semi-quantitative measurement is also possible using aqueous standards for calibration. Rapid measurement of sorbed analyte ions on the biological cells without separation of the cells from the original suspension is demonstrated in kinetic study of the interactions of metal ions and algae in aqueous standards. © 2010 The Royal Society of Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/168465
ISSN
2015 Impact Factor: 3.379
2015 SCImago Journal Rankings: 1.022
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, KSen_US
dc.contributor.authorChan, WTen_US
dc.date.accessioned2012-10-08T03:19:17Z-
dc.date.available2012-10-08T03:19:17Z-
dc.date.issued2010en_US
dc.identifier.citationJournal Of Analytical Atomic Spectrometry, 2010, v. 25 n. 7, p. 1114-1122en_US
dc.identifier.issn0267-9477en_US
dc.identifier.urihttp://hdl.handle.net/10722/168465-
dc.description.abstractA method of simultaneous cell counting and determination of constituent metals in single cells using time-resolved inductively coupled plasma-mass spectrometry (ICP-MS) is reported. A unicellular alga, Chlorella vulgaris, was used as a model in this study. Algae suspensions of number density of 10 6/mL were converted into fine aerosols using conventional solution nebulization for sample introduction into the ICP. The metals in each cell are converted into a plume of gaseous analyte ions in the ICP. Each metal in the plume produces a current spike at the detector of the mass spectrometer. ICP-MS intensity spikes of major elements, such as Mg of 108 atoms/cell, and trace elements, such as Mn and Cu of 5 × 106 atoms/cell, were detected readily. The number density of the cells in an algae suspension can be determined by counting the number of the ICP-MS intensity spikes of the major element (Mg) over a fixed period of time. The uncertainty in cell counting was approximately 2% RSD, similar to that of hemacytometry. The ICP-MS spike intensity of an element is related to its quantity in the algal cell. Quantitative determination of the metal contents of the algal cells using metal oxide particles for calibration is feasible. Semi-quantitative measurement is also possible using aqueous standards for calibration. Rapid measurement of sorbed analyte ions on the biological cells without separation of the cells from the original suspension is demonstrated in kinetic study of the interactions of metal ions and algae in aqueous standards. © 2010 The Royal Society of Chemistry.en_US
dc.languageengen_US
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/jaasen_US
dc.relation.ispartofJournal of Analytical Atomic Spectrometryen_US
dc.titleTime-resolved ICP-MS measurement for single-cell analysis and on-line cytometryen_US
dc.typeArticleen_US
dc.identifier.emailChan, WT:wtchan@hku.hken_US
dc.identifier.authorityChan, WT=rp00668en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1039/c002272aen_US
dc.identifier.scopuseid_2-s2.0-77953853935en_US
dc.identifier.hkuros174884-
dc.identifier.hkuros232298-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953853935&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume25en_US
dc.identifier.issue7en_US
dc.identifier.spage1114en_US
dc.identifier.epage1122en_US
dc.identifier.eissn1364-5544-
dc.identifier.isiWOS:000279099000025-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHo, KS=36162334100en_US
dc.identifier.scopusauthoridChan, WT=7403918827en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats