File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

TitleIdentification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry
Authors
Keywords5-Fluorouracil Response Proteins
Colorectal Cancer
Mass Spectrometry
Sw480
Two-Dimensional Electrophoresis
Issue Date2008
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htm
Citation
Oncology Reports, 2008, v. 20 n. 1, p. 89-98 How to Cite?
AbstractColorectal cancer (CRC) is the second most prevalent cause of cancer-related deaths in the Western world. 5-Fluorouracil (5-FU) is a standard chemotherapeutic drug to treat CRC. However, the response rate is less than 20% and patients who have responded to 5-FU may become resistant. Therefore there is an urgent need to examine the 5-FU response proteins so that patients with no response to 5-FU can change to other treatment strategies promptly. In this study, the proteomic expression profile in a CRC cell line SW480 before and after 5-FU treatment was examined using 2-dimensional electrophoresis technology. Fourteen proteins with differential expression were identified using mass spectrometry and 7 of them were validated using immunocytochemical (ICC) staining. Protein identification indicated that cyclophilin A, cytokeratin 19 (CK19), cytokeratin 8 (CK8), ras-related nuclear protein, heat shock protein 27 (hsp27) and peroxiredoxin 6 (Prx 6) were upregulated whereas heat shock protein 60 (hsp60), cytokeratin 18 (CK18), cytokeratin 9 (CK9), carbamoylphosphate synthetase I, α-enolase, heat shock protein 70 (hsp70), nm23 and β-actin were down-regulated. Seven of the 14 proteins detected were validated by ICC staining, which showed that the expression of hsp27, Prx 6 and hsp70 correlated with that from proteomics profiling. Our results suggest that hsp27, Prx 6 and hsp70 are potential 5-FU response proteins and they may represent potential targets for further evaluation in other 5-FU-sensitive and -resistant CRC cell lines.
Persistent Identifierhttp://hdl.handle.net/10722/168320
ISSN
2015 Impact Factor: 2.486
2015 SCImago Journal Rankings: 0.968
References

 

DC FieldValueLanguage
dc.contributor.authorWong, CSZen_US
dc.contributor.authorWaiKi Wong, Ven_US
dc.contributor.authorMingLok Chan, Cen_US
dc.contributor.authorBuigYue Ma, Ben_US
dc.contributor.authorPun Hui, Een_US
dc.contributor.authorChiKeung Wong, Men_US
dc.contributor.authorYanYee Lam, Men_US
dc.contributor.authorChiChuen, Ten_US
dc.contributor.authorChan, WHen_US
dc.contributor.authorCheuk, Wen_US
dc.contributor.authorTakCheung Chan, Aen_US
dc.date.accessioned2012-10-08T03:17:32Z-
dc.date.available2012-10-08T03:17:32Z-
dc.date.issued2008en_US
dc.identifier.citationOncology Reports, 2008, v. 20 n. 1, p. 89-98en_US
dc.identifier.issn1021-335Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/168320-
dc.description.abstractColorectal cancer (CRC) is the second most prevalent cause of cancer-related deaths in the Western world. 5-Fluorouracil (5-FU) is a standard chemotherapeutic drug to treat CRC. However, the response rate is less than 20% and patients who have responded to 5-FU may become resistant. Therefore there is an urgent need to examine the 5-FU response proteins so that patients with no response to 5-FU can change to other treatment strategies promptly. In this study, the proteomic expression profile in a CRC cell line SW480 before and after 5-FU treatment was examined using 2-dimensional electrophoresis technology. Fourteen proteins with differential expression were identified using mass spectrometry and 7 of them were validated using immunocytochemical (ICC) staining. Protein identification indicated that cyclophilin A, cytokeratin 19 (CK19), cytokeratin 8 (CK8), ras-related nuclear protein, heat shock protein 27 (hsp27) and peroxiredoxin 6 (Prx 6) were upregulated whereas heat shock protein 60 (hsp60), cytokeratin 18 (CK18), cytokeratin 9 (CK9), carbamoylphosphate synthetase I, α-enolase, heat shock protein 70 (hsp70), nm23 and β-actin were down-regulated. Seven of the 14 proteins detected were validated by ICC staining, which showed that the expression of hsp27, Prx 6 and hsp70 correlated with that from proteomics profiling. Our results suggest that hsp27, Prx 6 and hsp70 are potential 5-FU response proteins and they may represent potential targets for further evaluation in other 5-FU-sensitive and -resistant CRC cell lines.en_US
dc.languageengen_US
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htmen_US
dc.relation.ispartofOncology Reportsen_US
dc.subject5-Fluorouracil Response Proteinsen_US
dc.subjectColorectal Canceren_US
dc.subjectMass Spectrometryen_US
dc.subjectSw480en_US
dc.subjectTwo-Dimensional Electrophoresisen_US
dc.titleIdentification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometryen_US
dc.typeArticleen_US
dc.identifier.emailChan, WH:waichan@hku.hken_US
dc.identifier.authorityChan, WH=rp00667en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-49849090629en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49849090629&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue1en_US
dc.identifier.spage89en_US
dc.identifier.epage98en_US
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridWong, CSZ=26039647800en_US
dc.identifier.scopusauthoridWaiKi Wong, V=25626846000en_US
dc.identifier.scopusauthoridMingLok Chan, C=25626783000en_US
dc.identifier.scopusauthoridBuigYue Ma, B=7403301016en_US
dc.identifier.scopusauthoridPun Hui, E=7005081895en_US
dc.identifier.scopusauthoridChiKeung Wong, M=25626292900en_US
dc.identifier.scopusauthoridYanYee Lam, M=25626915200en_US
dc.identifier.scopusauthoridChiChuen, T=25626079400en_US
dc.identifier.scopusauthoridChan, WH=13310083000en_US
dc.identifier.scopusauthoridCheuk, W=7003958432en_US
dc.identifier.scopusauthoridTakCheung Chan, A=25626859700en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats