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- Publisher Website: 10.1038/nsb948
- Scopus: eid_2-s2.0-0042123525
- PMID: 12833153
- WOS: WOS:000184412400014
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Article: Structure of the LpxC deacetylase with a bound substrate-analog inhibitor
Title | Structure of the LpxC deacetylase with a bound substrate-analog inhibitor |
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Authors | |
Issue Date | 2003 |
Citation | Nature Structural Biology, 2003, v. 10 n. 8, p. 645-651 How to Cite? |
Abstract | The zinc-dependent UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) catalyzes the first committed step in the biosynthesis of lipid A, the hydrophobic anchor of lipopolysaccharide (LPS) that constitutes the outermost monolayer of Gram-negative bacteria. As LpxC is crucial for the survival of Gram-negative organisms and has no sequence homology to known mammalian deacetylases or amidases, it is an excellent target for the design of new antibiotics. The solution structure of LpxC from Aquifex aeolicus in complex with a substrate-analog inhibitor, TU-514, reveals a novel α/β fold, a unique zinc-binding motif and a hydrophobic passage that captures the acyl chain of the inhibitor. On the basis of biochemical and structural studies, we propose a catalytic mechanism for LpxC, suggest a model for substrate binding and provide evidence that mobility and dynamics in structural motifs close to the active site have key roles in the capture of the substrate. |
Persistent Identifier | http://hdl.handle.net/10722/167822 |
ISSN | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Coggins, BE | en_US |
dc.contributor.author | Li, X | en_US |
dc.contributor.author | Mcclerren, AL | en_US |
dc.contributor.author | Hindsgaul, O | en_US |
dc.contributor.author | Raetz, CRH | en_US |
dc.contributor.author | Zhou, P | en_US |
dc.date.accessioned | 2012-10-08T03:11:59Z | - |
dc.date.available | 2012-10-08T03:11:59Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Nature Structural Biology, 2003, v. 10 n. 8, p. 645-651 | en_US |
dc.identifier.issn | 1072-8368 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167822 | - |
dc.description.abstract | The zinc-dependent UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) catalyzes the first committed step in the biosynthesis of lipid A, the hydrophobic anchor of lipopolysaccharide (LPS) that constitutes the outermost monolayer of Gram-negative bacteria. As LpxC is crucial for the survival of Gram-negative organisms and has no sequence homology to known mammalian deacetylases or amidases, it is an excellent target for the design of new antibiotics. The solution structure of LpxC from Aquifex aeolicus in complex with a substrate-analog inhibitor, TU-514, reveals a novel α/β fold, a unique zinc-binding motif and a hydrophobic passage that captures the acyl chain of the inhibitor. On the basis of biochemical and structural studies, we propose a catalytic mechanism for LpxC, suggest a model for substrate binding and provide evidence that mobility and dynamics in structural motifs close to the active site have key roles in the capture of the substrate. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Nature Structural Biology | en_US |
dc.subject.mesh | Amidohydrolases - Antagonists & Inhibitors - Chemistry - Genetics | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Anti-Bacterial Agents - Chemistry - Pharmacology | en_US |
dc.subject.mesh | Catalytic Domain | en_US |
dc.subject.mesh | Drug Design | en_US |
dc.subject.mesh | Enzyme Inhibitors - Chemistry - Pharmacology | en_US |
dc.subject.mesh | Escherichia Coli - Enzymology - Genetics | en_US |
dc.subject.mesh | Gram-Negative Bacteria - Enzymology - Genetics | en_US |
dc.subject.mesh | Models, Molecular | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Nuclear Magnetic Resonance, Biomolecular | en_US |
dc.subject.mesh | Protein Conformation | en_US |
dc.subject.mesh | Recombinant Proteins - Antagonists & Inhibitors - Chemistry - Genetics | en_US |
dc.subject.mesh | Sequence Homology, Amino Acid | en_US |
dc.subject.mesh | Substrate Specificity | en_US |
dc.subject.mesh | Zinc - Chemistry | en_US |
dc.title | Structure of the LpxC deacetylase with a bound substrate-analog inhibitor | en_US |
dc.type | Article | en_US |
dc.identifier.email | Li, X:xuechenl@hku.hk | en_US |
dc.identifier.authority | Li, X=rp00742 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1038/nsb948 | en_US |
dc.identifier.pmid | 12833153 | - |
dc.identifier.scopus | eid_2-s2.0-0042123525 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0042123525&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 10 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.spage | 645 | en_US |
dc.identifier.epage | 651 | en_US |
dc.identifier.isi | WOS:000184412400014 | - |
dc.identifier.f1000 | 1015024 | - |
dc.identifier.scopusauthorid | Coggins, BE=6507075734 | en_US |
dc.identifier.scopusauthorid | Li, X=24168958800 | en_US |
dc.identifier.scopusauthorid | McClerren, AL=7801327115 | en_US |
dc.identifier.scopusauthorid | Hindsgaul, O=7102966220 | en_US |
dc.identifier.scopusauthorid | Raetz, CRH=7102514726 | en_US |
dc.identifier.scopusauthorid | Zhou, P=7401848622 | en_US |
dc.identifier.issnl | 1072-8368 | - |