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Article: Identification of the site of inhibition of mitogenic signaling by oncogenic ras-p21 by a ras effector peptide

TitleIdentification of the site of inhibition of mitogenic signaling by oncogenic ras-p21 by a ras effector peptide
Authors
Issue Date2002
Citation
Journal Of Protein Chemistry, 2002, v. 21 n. 5, p. 367-370 How to Cite?
AbstractWe have previously found that a ras switch 1 domain peptide (PNC-7, residues 35-47) selectively blocks oocyte maturation induced by oncogenic (Val 12-containing) ras-p21 protein and also blocks c-raf-induced oocyte maturation. We now find that oncogenic p21 does not inhibit oocyte maturation of a constitutively activated raf protein (raf BXB) that is lacking most of the first 301 amino terminal amino acids, including the major ras binding domain and accessory ras-binding regions. We also find that a dominant negative raf that completely blocks c-raf-induced maturation likewise does not block raf-BXB-induced maturation. We conclude that PNC-7 blocks ras by binding to the amino-terminal domain of raf and that raf BXB must initiate signal transduction in the cytosol.
Persistent Identifierhttp://hdl.handle.net/10722/167739
ISSN
2005 Impact Factor: 1.487
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChie, Len_US
dc.contributor.authorFriedman, FKen_US
dc.contributor.authorKung, HFen_US
dc.contributor.authorLin, MCMen_US
dc.contributor.authorChung, Den_US
dc.contributor.authorPincus, MRen_US
dc.date.accessioned2012-10-08T03:10:49Z-
dc.date.available2012-10-08T03:10:49Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Protein Chemistry, 2002, v. 21 n. 5, p. 367-370en_US
dc.identifier.issn0277-8033en_US
dc.identifier.urihttp://hdl.handle.net/10722/167739-
dc.description.abstractWe have previously found that a ras switch 1 domain peptide (PNC-7, residues 35-47) selectively blocks oocyte maturation induced by oncogenic (Val 12-containing) ras-p21 protein and also blocks c-raf-induced oocyte maturation. We now find that oncogenic p21 does not inhibit oocyte maturation of a constitutively activated raf protein (raf BXB) that is lacking most of the first 301 amino terminal amino acids, including the major ras binding domain and accessory ras-binding regions. We also find that a dominant negative raf that completely blocks c-raf-induced maturation likewise does not block raf-BXB-induced maturation. We conclude that PNC-7 blocks ras by binding to the amino-terminal domain of raf and that raf BXB must initiate signal transduction in the cytosol.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Protein Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshEnzyme Inhibitors - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshMitogens - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshOncogene Protein P21(Ras) - Antagonists & Inhibitors - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshOocytes - Drug Effects - Growth & Developmenten_US
dc.subject.meshPeptide Fragments - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshProto-Oncogene Proteins C-Raf - Antagonists & Inhibitors - Chemistry - Genetics - Metabolismen_US
dc.subject.meshSignal Transduction - Drug Effectsen_US
dc.subject.meshXenopus Laevisen_US
dc.titleIdentification of the site of inhibition of mitogenic signaling by oncogenic ras-p21 by a ras effector peptideen_US
dc.typeArticleen_US
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MCM=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/A:1019998403181en_US
dc.identifier.pmid12206511-
dc.identifier.scopuseid_2-s2.0-0036637644en_US
dc.identifier.hkuros79190-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036637644&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume21en_US
dc.identifier.issue5en_US
dc.identifier.spage367en_US
dc.identifier.epage370en_US
dc.identifier.isiWOS:000177629500007-
dc.identifier.scopusauthoridChie, L=6602998028en_US
dc.identifier.scopusauthoridFriedman, FK=7101617312en_US
dc.identifier.scopusauthoridKung, HF=7402514190en_US
dc.identifier.scopusauthoridLin, MCM=7404816359en_US
dc.identifier.scopusauthoridChung, D=7401719222en_US
dc.identifier.scopusauthoridPincus, MR=7101995825en_US

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