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Article: The oil-drop tensiometer: Potential applications for studying the kinetics of (phospho)lipase action

TitleThe oil-drop tensiometer: Potential applications for studying the kinetics of (phospho)lipase action
Authors
KeywordsBarostat
Hyperbar
Interfacial Tension
Lipase Kinetics
Oil Drop
Triacylglycerol
Issue Date1994
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/chemphyslip
Citation
Chemistry And Physics Of Lipids, 1994, v. 71 n. 2, p. 163-173 How to Cite?
AbstractIn 1987, Nury et al. adapted the well-known oil-drop technique for measuring interfacial tension for the purpose of monitoring the lipase hydrolysis of natural long-chain triacylglycerols. On the basis of this initial study, we developed an automated, digitised, computer-driven device with which we further prospected for applications of the oil-drop tensiometer in studies on lipolytic enzyme kinetics. In the present study, we first established that the new device provided reliable interfacial tension measurements, similar to those previously published. Furthermore, by keeping the oil-water interfacial tension at a fixed end-point value, the enzyme kinetics can be monitored using the change with time in the area of the oil drop. We then describe specific applications involving: (i) the measurement of lipase kinetics using minute amounts of enzyme; we checked the existence of a linear relationship between the initial decrease in the interfacial tension and the lipase concentration and found that the initial rates did not vary significantly between several successive drops formed within a 20-min period in the same lipase solution; (ii) the kinetic assay of phospholipase A 2 and (iii) studying the effects of high-pressure conditions upon lipase activity.
Persistent Identifierhttp://hdl.handle.net/10722/167520
ISSN
2015 Impact Factor: 2.901
2015 SCImago Journal Rankings: 0.971
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLabourdenne, Sen_US
dc.contributor.authorGaudryRolland, Nen_US
dc.contributor.authorLetellier, Sen_US
dc.contributor.authorLin, Men_US
dc.contributor.authorCagna, Aen_US
dc.contributor.authorEsposito, Gen_US
dc.contributor.authorVerger, Ren_US
dc.contributor.authorRivière, Cen_US
dc.date.accessioned2012-10-08T03:08:00Z-
dc.date.available2012-10-08T03:08:00Z-
dc.date.issued1994en_US
dc.identifier.citationChemistry And Physics Of Lipids, 1994, v. 71 n. 2, p. 163-173en_US
dc.identifier.issn0009-3084en_US
dc.identifier.urihttp://hdl.handle.net/10722/167520-
dc.description.abstractIn 1987, Nury et al. adapted the well-known oil-drop technique for measuring interfacial tension for the purpose of monitoring the lipase hydrolysis of natural long-chain triacylglycerols. On the basis of this initial study, we developed an automated, digitised, computer-driven device with which we further prospected for applications of the oil-drop tensiometer in studies on lipolytic enzyme kinetics. In the present study, we first established that the new device provided reliable interfacial tension measurements, similar to those previously published. Furthermore, by keeping the oil-water interfacial tension at a fixed end-point value, the enzyme kinetics can be monitored using the change with time in the area of the oil drop. We then describe specific applications involving: (i) the measurement of lipase kinetics using minute amounts of enzyme; we checked the existence of a linear relationship between the initial decrease in the interfacial tension and the lipase concentration and found that the initial rates did not vary significantly between several successive drops formed within a 20-min period in the same lipase solution; (ii) the kinetic assay of phospholipase A 2 and (iii) studying the effects of high-pressure conditions upon lipase activity.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/chemphyslipen_US
dc.relation.ispartofChemistry and Physics of Lipidsen_US
dc.subjectBarostaten_US
dc.subjectHyperbaren_US
dc.subjectInterfacial Tensionen_US
dc.subjectLipase Kineticsen_US
dc.subjectOil Dropen_US
dc.subjectTriacylglycerolen_US
dc.titleThe oil-drop tensiometer: Potential applications for studying the kinetics of (phospho)lipase actionen_US
dc.typeArticleen_US
dc.identifier.emailLin, M:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, M=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0009-3084(94)90068-Xen_US
dc.identifier.scopuseid_2-s2.0-0028338845en_US
dc.identifier.volume71en_US
dc.identifier.issue2en_US
dc.identifier.spage163en_US
dc.identifier.epage173en_US
dc.identifier.isiWOS:A1994NU69100004-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridLabourdenne, S=6507278492en_US
dc.identifier.scopusauthoridGaudryRolland, N=6505738369en_US
dc.identifier.scopusauthoridLetellier, S=16157681000en_US
dc.identifier.scopusauthoridLin, M=7404816359en_US
dc.identifier.scopusauthoridCagna, A=6603235005en_US
dc.identifier.scopusauthoridEsposito, G=16156060800en_US
dc.identifier.scopusauthoridVerger, R=7102853507en_US
dc.identifier.scopusauthoridRivière, C=7005624039en_US

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