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Article: Induction of glucagon responsiveness in transformed MDCK cells unresponsive to glucagon

TitleInduction of glucagon responsiveness in transformed MDCK cells unresponsive to glucagon
Other Titles[30] Induction of glucagon responsiveness in transformed MDCK cells unresponsive to glucagon
Authors
Issue Date1985
PublisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879
Citation
Methods In Enzymology, 1985, v. 109, p. 356-360 How to Cite?
AbstractThis chapter discusses a cloned line of Madin–Darby canine kidney (MDCK) cells, which were transformed with Harvey murine sarcoma virus. This line is maintained in continuous culture under the same conditions as the parental MDCK cells in Dulbecco's MEM, 5% fetal bovine serum, 5% CO2/95% air, with 80% humidity. The morphology of this transformed line is more fibroblastic than that of normal cells; in addition, a 21,000 Da protein (p21) coded by the virus has been identified on the inner surface of the plasma membrane of this transformed line. The growth characteristics of transformed MDCK cells are similar to normal cells; both grow equally well in serum free media. Like normal MDCK cells, the adenylate cyclase of the transformed line responds to a variety of hormones. However, transformation results in a selective loss of glucagon responsiveness because of the absence of glucagon binding sites at the cell surface. Thus, this cell line represents a good model system to examine factors that regulate the expression of differentiated functions.
Persistent Identifierhttp://hdl.handle.net/10722/167463
ISSN
2014 Impact Factor: 2.088
2015 SCImago Journal Rankings: 1.501

 

DC FieldValueLanguage
dc.contributor.authorBeckner, SKen_US
dc.contributor.authorDarfler, FJen_US
dc.contributor.authorLin, MCen_US
dc.date.accessioned2012-10-08T03:07:20Z-
dc.date.available2012-10-08T03:07:20Z-
dc.date.issued1985en_US
dc.identifier.citationMethods In Enzymology, 1985, v. 109, p. 356-360en_US
dc.identifier.issn0076-6879en_US
dc.identifier.urihttp://hdl.handle.net/10722/167463-
dc.description.abstractThis chapter discusses a cloned line of Madin–Darby canine kidney (MDCK) cells, which were transformed with Harvey murine sarcoma virus. This line is maintained in continuous culture under the same conditions as the parental MDCK cells in Dulbecco's MEM, 5% fetal bovine serum, 5% CO2/95% air, with 80% humidity. The morphology of this transformed line is more fibroblastic than that of normal cells; in addition, a 21,000 Da protein (p21) coded by the virus has been identified on the inner surface of the plasma membrane of this transformed line. The growth characteristics of transformed MDCK cells are similar to normal cells; both grow equally well in serum free media. Like normal MDCK cells, the adenylate cyclase of the transformed line responds to a variety of hormones. However, transformation results in a selective loss of glucagon responsiveness because of the absence of glucagon binding sites at the cell surface. Thus, this cell line represents a good model system to examine factors that regulate the expression of differentiated functions.-
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879en_US
dc.relation.ispartofMethods in Enzymologyen_US
dc.titleInduction of glucagon responsiveness in transformed MDCK cells unresponsive to glucagonen_US
dc.title.alternative[30] Induction of glucagon responsiveness in transformed MDCK cells unresponsive to glucagon-
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0076-6879(85)09100-5-
dc.identifier.scopuseid_2-s2.0-0021893843en_US
dc.identifier.volume109en_US
dc.identifier.spage356en_US
dc.identifier.epage360en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBeckner, SK=7004064238en_US
dc.identifier.scopusauthoridDarfler, FJ=6603120994en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US

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