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Article: Inhibition of cholera toxin activation of the adenylate cyclase system in intact HeLa cells

TitleInhibition of cholera toxin activation of the adenylate cyclase system in intact HeLa cells
Authors
Issue Date1980
Citation
Journal Of Cyclic Nucleotide Research, 1980, v. 6 n. 5, p. 359-367 How to Cite?
AbstractCholera toxin treatment activates the adenylate cyclase in intact HeLa cells. However, pretreatment of the cells with chemicals known to inhibit receptor internalization and lysosomal processing blocks the toxin include methylamine, ammonium chloride, chloroquine and dansylcadaverine. These chemicals did not affect either the binding of ( 125I)-cholera toxin to HeLa cells nor the ability of A 1 peptide to activate the adenylate cyclase in plasma membrane preparations. We conclude that these chemicals act on the processing of the toxin subsequent to its binding and that internalization and lysosomal processing mediate the release of the active fragment from cholera toxin, which activates the adenylate cyclase system.
Persistent Identifierhttp://hdl.handle.net/10722/167451
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLin, MCen_US
dc.contributor.authorTaniuchi, Men_US
dc.date.accessioned2012-10-08T03:07:10Z-
dc.date.available2012-10-08T03:07:10Z-
dc.date.issued1980en_US
dc.identifier.citationJournal Of Cyclic Nucleotide Research, 1980, v. 6 n. 5, p. 359-367en_US
dc.identifier.issn0095-1544en_US
dc.identifier.urihttp://hdl.handle.net/10722/167451-
dc.description.abstractCholera toxin treatment activates the adenylate cyclase in intact HeLa cells. However, pretreatment of the cells with chemicals known to inhibit receptor internalization and lysosomal processing blocks the toxin include methylamine, ammonium chloride, chloroquine and dansylcadaverine. These chemicals did not affect either the binding of ( 125I)-cholera toxin to HeLa cells nor the ability of A 1 peptide to activate the adenylate cyclase in plasma membrane preparations. We conclude that these chemicals act on the processing of the toxin subsequent to its binding and that internalization and lysosomal processing mediate the release of the active fragment from cholera toxin, which activates the adenylate cyclase system.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Cyclic Nucleotide Researchen_US
dc.subject.meshAdenylate Cyclase - Metabolismen_US
dc.subject.meshAmmonium Chloride - Pharmacologyen_US
dc.subject.meshCadaverine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshChloroquine - Pharmacologyen_US
dc.subject.meshCholera Toxin - Antagonists & Inhibitors - Metabolismen_US
dc.subject.meshEnzyme Activation - Drug Effectsen_US
dc.subject.meshG(M1) Gangliosideen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshMethylamines - Pharmacologyen_US
dc.subject.meshReceptors, Cell Surfaceen_US
dc.subject.meshReceptors, Immunologic - Antagonists & Inhibitorsen_US
dc.titleInhibition of cholera toxin activation of the adenylate cyclase system in intact HeLa cellsen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7217459-
dc.identifier.scopuseid_2-s2.0-0019297732en_US
dc.identifier.volume6en_US
dc.identifier.issue5en_US
dc.identifier.spage359en_US
dc.identifier.epage367en_US
dc.identifier.isiWOS:A1980LC39900003-
dc.identifier.scopusauthoridLin, MC=7404816359en_US
dc.identifier.scopusauthoridTaniuchi, M=6701625278en_US

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