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Article: Determination of amino acids and proteins by dual-electrode detection in a flow system

TitleDetermination of amino acids and proteins by dual-electrode detection in a flow system
Authors
Issue Date1995
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 1995, v. 67 n. 6, p. 1121-1124 How to Cite?
AbstractA new analytical procedure using a serial dual-electrode detector was developed for amino acid and protein analysis. Bromine was generated at die upstream electrode and detected by the downstream electrode. Amino acids and proteins present in the samples were shown to lower the downstream current but had no apparent effect on me upstream current. The potential of both electrodes, the flow rate of the mobile electrolyte, and the dispersion of the samples were optimized to give satisfactory linear working ranges for seven amino acids (10-6-10-3 M) and total proteins (10-7-10-3 M). The working range can be extended up to 10-1 M amino acid and 10-2 M protein by using a more anodic upstream potential. Direct determination without preconcentration can thus be performed on all the real food samples analyzed. This method has been applied to the flow injection analysis of total proteins in food, with results comparable to those obtained using the established AOAC procedure. The dual-electrode detector provides a suitable method for detecting analytes after HPLC separation. Compared to the traditional method with UV detection, the dual-electrode detector is shown to provide a better selectivity for the detection of ribonuclease and catalase in food.
Persistent Identifierhttp://hdl.handle.net/10722/167260
ISSN
2015 Impact Factor: 5.886
2015 SCImago Journal Rankings: 2.369
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFung, YSen_US
dc.contributor.authorMo, SYen_US
dc.date.accessioned2012-10-08T03:04:57Z-
dc.date.available2012-10-08T03:04:57Z-
dc.date.issued1995en_US
dc.identifier.citationAnalytical Chemistry, 1995, v. 67 n. 6, p. 1121-1124en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://hdl.handle.net/10722/167260-
dc.description.abstractA new analytical procedure using a serial dual-electrode detector was developed for amino acid and protein analysis. Bromine was generated at die upstream electrode and detected by the downstream electrode. Amino acids and proteins present in the samples were shown to lower the downstream current but had no apparent effect on me upstream current. The potential of both electrodes, the flow rate of the mobile electrolyte, and the dispersion of the samples were optimized to give satisfactory linear working ranges for seven amino acids (10-6-10-3 M) and total proteins (10-7-10-3 M). The working range can be extended up to 10-1 M amino acid and 10-2 M protein by using a more anodic upstream potential. Direct determination without preconcentration can thus be performed on all the real food samples analyzed. This method has been applied to the flow injection analysis of total proteins in food, with results comparable to those obtained using the established AOAC procedure. The dual-electrode detector provides a suitable method for detecting analytes after HPLC separation. Compared to the traditional method with UV detection, the dual-electrode detector is shown to provide a better selectivity for the detection of ribonuclease and catalase in food.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_US
dc.relation.ispartofAnalytical Chemistryen_US
dc.titleDetermination of amino acids and proteins by dual-electrode detection in a flow systemen_US
dc.typeArticleen_US
dc.identifier.emailFung, YS:ysfung@hku.hken_US
dc.identifier.authorityFung, YS=rp00697en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ac00102a016-
dc.identifier.scopuseid_2-s2.0-0000249165en_US
dc.identifier.hkuros1286-
dc.identifier.volume67en_US
dc.identifier.issue6en_US
dc.identifier.spage1121en_US
dc.identifier.epage1124en_US
dc.identifier.isiWOS:A1995QM00100016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFung, YS=13309754700en_US
dc.identifier.scopusauthoridMo, SY=7004957705en_US

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