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Article: Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
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TitleEvaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
 
AuthorsTse, M1
Kim, M2
Chan, CH1
Ho, PL1
Ma, SK1
Guan, Y1
Peiris, JSM1
 
Issue Date2012
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
 
CitationClinical And Vaccine Immunology, 2012, v. 19 n. 3, p. 334-337 [How to Cite?]
DOI: http://dx.doi.org/10.1128/CVI.05358-11
 
AbstractThe reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
 
ISSN1556-6811
2012 Impact Factor: 2.598
2012 SCImago Journal Rankings: 0.938
 
DOIhttp://dx.doi.org/10.1128/CVI.05358-11
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTse, M
 
dc.contributor.authorKim, M
 
dc.contributor.authorChan, CH
 
dc.contributor.authorHo, PL
 
dc.contributor.authorMa, SK
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorPeiris, JSM
 
dc.date.accessioned2012-09-20T08:48:15Z
 
dc.date.available2012-09-20T08:48:15Z
 
dc.date.issued2012
 
dc.description.abstractThe reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical And Vaccine Immunology, 2012, v. 19 n. 3, p. 334-337 [How to Cite?]
DOI: http://dx.doi.org/10.1128/CVI.05358-11
 
dc.identifier.doihttp://dx.doi.org/10.1128/CVI.05358-11
 
dc.identifier.epage337
 
dc.identifier.hkuros211311
 
dc.identifier.issn1556-6811
2012 Impact Factor: 2.598
2012 SCImago Journal Rankings: 0.938
 
dc.identifier.issue3
 
dc.identifier.pmid22219314
 
dc.identifier.scopuseid_2-s2.0-84863247182
 
dc.identifier.spage334
 
dc.identifier.urihttp://hdl.handle.net/10722/166794
 
dc.identifier.volume19
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical and Vaccine Immunology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAntibodies, Viral - blood
 
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methods
 
dc.subject.meshInfluenza A Virus, H1N1 Subtype - immunology
 
dc.subject.meshOrthomyxoviridae Infections - diagnosis - immunology - virology
 
dc.subject.meshROC Curve
 
dc.subject.meshSensitivity and Specificity
 
dc.subject.meshSeroepidemiologic Studies
 
dc.subject.meshSwine
 
dc.subject.meshSwine Diseases - diagnosis - immunology - virology
 
dc.titleEvaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
 
dc.typeArticle
 
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<contributor.author>Guan, Y</contributor.author>
<contributor.author>Peiris, JSM</contributor.author>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Food and Agriculture Organization of the United Nations