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Article: Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs

TitleEvaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
Authors
Issue Date2012
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
Citation
Clinical And Vaccine Immunology, 2012, v. 19 n. 3, p. 334-337 How to Cite?
AbstractThe reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/166794
ISSN
2014 Impact Factor: 2.470
2014 SCImago Journal Rankings: 1.157
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, Men_HK
dc.contributor.authorKim, Men_HK
dc.contributor.authorChan, CHen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorMa, SKen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2012-09-20T08:48:15Z-
dc.date.available2012-09-20T08:48:15Z-
dc.date.issued2012en_HK
dc.identifier.citationClinical And Vaccine Immunology, 2012, v. 19 n. 3, p. 334-337en_HK
dc.identifier.issn1556-6811en_HK
dc.identifier.urihttp://hdl.handle.net/10722/166794-
dc.description.abstractThe reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively. Copyright © 2012, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/ en_HK
dc.relation.ispartofClinical and Vaccine Immunologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntibodies, Viral - blooden_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methodsen_HK
dc.subject.meshInfluenza A Virus, H1N1 Subtype - immunologyen_HK
dc.subject.meshOrthomyxoviridae Infections - diagnosis - immunology - virologyen_HK
dc.subject.meshROC Curveen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSeroepidemiologic Studiesen_HK
dc.subject.meshSwineen_HK
dc.subject.meshSwine Diseases - diagnosis - immunology - virologyen_HK
dc.titleEvaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigsen_HK
dc.typeArticleen_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/CVI.05358-11en_HK
dc.identifier.pmid22219314en_HK
dc.identifier.scopuseid_2-s2.0-84863247182en_HK
dc.identifier.hkuros211311en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863247182&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue3en_HK
dc.identifier.spage334en_HK
dc.identifier.epage337en_HK
dc.identifier.isiWOS:000300841200006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTse, M=55278130600en_HK
dc.identifier.scopusauthoridKim, M=55278074400en_HK
dc.identifier.scopusauthoridChan, CH=55276514900en_HK
dc.identifier.scopusauthoridHo, PL=55276473900en_HK
dc.identifier.scopusauthoridMa, SK=55277726300en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK

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