Article: Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs

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- Professor Peiris, Joseph Sriyal Malik
- Professor Guan, Yi

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Publisher Website: 10.1128/CVI.05358-11
Scopus: eid_2-s2.0-84863247182
PMID: 22219314

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Title	Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
Authors	Tse, M1 Kim, M2 Chan, CH1 Ho, PL1 Ma, SK1 Guan, Y1 Peiris, JSM1
Issue Date	2012
Publisher	American Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
Citation	Clinical And Vaccine Immunology, 2012, v. 19 n. 3, p. 334-337 [How to Cite?] DOI: http://dx.doi.org/10.1128/CVI.05358-11
Abstract	The reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
ISSN	1556-6811 2011 Impact Factor: 2.546 2011 SCImago Journal Rankings: 0.248
DOI	http://dx.doi.org/10.1128/CVI.05358-11
References	References in Scopus

DC Field	Value
dc.contributor.author	Tse, M
dc.contributor.author	Kim, M
dc.contributor.author	Chan, CH
dc.contributor.author	Ho, PL
dc.contributor.author	Ma, SK
dc.contributor.author	Guan, Y
dc.contributor.author	Peiris, JSM
dc.date.accessioned	2012-09-20T08:48:15Z
dc.date.available	2012-09-20T08:48:15Z
dc.date.issued	2012
dc.description.abstract	The reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
dc.description.nature	Link_to_subscribed_fulltext
dc.identifier.citation	Clinical And Vaccine Immunology, 2012, v. 19 n. 3, p. 334-337 [How to Cite?] DOI: http://dx.doi.org/10.1128/CVI.05358-11
dc.identifier.doi	http://dx.doi.org/10.1128/CVI.05358-11
dc.identifier.epage	337
dc.identifier.hkuros	211311
dc.identifier.issn	1556-6811 2011 Impact Factor: 2.546 2011 SCImago Journal Rankings: 0.248
dc.identifier.issue	3
dc.identifier.pmid	22219314
dc.identifier.scopus	eid_2-s2.0-84863247182
dc.identifier.spage	334
dc.identifier.uri	http://hdl.handle.net/10722/166794
dc.identifier.volume	19
dc.language	eng
dc.publisher	American Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
dc.publisher.place	United States
dc.relation.ispartof	Clinical and Vaccine Immunology
dc.relation.references	References in Scopus
dc.subject.mesh	Animals
dc.subject.mesh	Antibodies, Viral - blood
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay - methods
dc.subject.mesh	Influenza A Virus, H1N1 Subtype - immunology
dc.subject.mesh	Orthomyxoviridae Infections - diagnosis - immunology - virology
dc.subject.mesh	ROC Curve
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Seroepidemiologic Studies
dc.subject.mesh	Swine
dc.subject.mesh	Swine Diseases - diagnosis - immunology - virology
dc.title	Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
dc.type	Article

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Author Affiliations

The University of Hong Kong Li Ka Shing Faculty of Medicine
Food and Agriculture Organization of the United Nations

Article: Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs

The HKU Scholars Hub has contact details for these author(s).