File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Extent of antigenic cross-reactivity among highly pathogenic H5N1 influenza viruses

TitleExtent of antigenic cross-reactivity among highly pathogenic H5N1 influenza viruses
Authors
Issue Date2011
PublisherAmerican Society for Microbiology.
Citation
Journal of Clinical Microbiology, 2011, v. 49 n. 10, p. 3531-3536 How to Cite?
AbstractHighly pathogenic H5N1 avian influenza viruses emerged in 1996 and have since evolved so extensively that a single strain can no longer be used as a prepandemic vaccine or diagnostic reagent. We therefore sought to identify the H5N1 strains that may best serve as cross-reactive diagnostic reagents. We compared the cross-reactivity of 27 viruses of clades 0, 1, 2.1, 2.2, 2.3, and 4 and of four computationally designed ancestral H5N1 strains by hemagglutination inhibition (HI) and microneutralization (MN) assays. Antigenic cartography was used to analyze the large quantity of resulting data. Cartographs of HI titers with chicken red blood cells were similar to those of MN titers, but HI with horse red blood cells decreased antigenic distances among the H5N1 strains studied. Thus, HI with horse red blood cells seems to be the assay of choice for H5N1 diagnostics. Whereas clade 2.2 antigens were able to detect antibodies raised to most of the tested H5N1 viruses (and clade 2.2-specific antisera detected most of the H5N1 antigens), ancestral strain A exhibited the widest reactivity pattern and hence was the best candidate diagnostic reagent for broad detection of H5N1 strains.
Persistent Identifierhttp://hdl.handle.net/10722/166791
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDucatez, MFen_US
dc.contributor.authorCai, Zen_US
dc.contributor.authorPeiris, Men_US
dc.contributor.authorGuan, Yen_US
dc.contributor.authorYe, Zen_US
dc.contributor.authorWan, XFen_US
dc.contributor.authorWebby, RJen_US
dc.date.accessioned2012-09-20T08:48:13Z-
dc.date.available2012-09-20T08:48:13Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Clinical Microbiology, 2011, v. 49 n. 10, p. 3531-3536en_US
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/166791-
dc.description.abstractHighly pathogenic H5N1 avian influenza viruses emerged in 1996 and have since evolved so extensively that a single strain can no longer be used as a prepandemic vaccine or diagnostic reagent. We therefore sought to identify the H5N1 strains that may best serve as cross-reactive diagnostic reagents. We compared the cross-reactivity of 27 viruses of clades 0, 1, 2.1, 2.2, 2.3, and 4 and of four computationally designed ancestral H5N1 strains by hemagglutination inhibition (HI) and microneutralization (MN) assays. Antigenic cartography was used to analyze the large quantity of resulting data. Cartographs of HI titers with chicken red blood cells were similar to those of MN titers, but HI with horse red blood cells decreased antigenic distances among the H5N1 strains studied. Thus, HI with horse red blood cells seems to be the assay of choice for H5N1 diagnostics. Whereas clade 2.2 antigens were able to detect antibodies raised to most of the tested H5N1 viruses (and clade 2.2-specific antisera detected most of the H5N1 antigens), ancestral strain A exhibited the widest reactivity pattern and hence was the best candidate diagnostic reagent for broad detection of H5N1 strains.-
dc.languageengen_US
dc.publisherAmerican Society for Microbiology.-
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.-
dc.rightsCopyright © American Society for Microbiology, [Journal of Clinical Microbiology, 2011, v. 49 n. 10, p. 3531-3536]-
dc.subject.meshAntibodies, Viral - immunology-
dc.subject.meshAntigens, Viral - immunology-
dc.subject.meshChickens-
dc.subject.meshCross Reactions-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - classification - immunology-
dc.titleExtent of antigenic cross-reactivity among highly pathogenic H5N1 influenza virusesen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, M: malik@hkucc.hku.hken_US
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_US
dc.identifier.emailWebby, RJ: richard.webby@stjude.org-
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.identifier.authorityGuan, Y=rp00397en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JCM.01279-11-
dc.identifier.pmid21832017-
dc.identifier.pmcidPMC3187336-
dc.identifier.scopuseid_2-s2.0-80053538503-
dc.identifier.hkuros211256en_US
dc.identifier.volume49en_US
dc.identifier.issue10en_US
dc.identifier.spage3531en_US
dc.identifier.epage3536en_US
dc.identifier.isiWOS:000295360700014-
dc.publisher.placeUnited States-
dc.identifier.scopusauthoridDucatez, MF=10044731300-
dc.identifier.scopusauthoridCai, Z=8941786700-
dc.identifier.scopusauthoridPeiris, M=7005486823-
dc.identifier.scopusauthoridGuan, Y=7202924055-
dc.identifier.scopusauthoridYe, Z=7401956778-
dc.identifier.scopusauthoridWan, XF=9335365800-
dc.identifier.scopusauthoridWebby, RJ=35448064800-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats