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Article: Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism

TitleBaicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism
Authors
KeywordsApoptosis
Cell structure
Cell viability
Controlled study
Cytotoxicity
Issue Date2012
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/home
Citation
Chinese Medicine, 2012, v. 7 n. 1, article no. 1 How to Cite?
AbstractBACKGROUND: Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism. METHODS: Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS) and loss of mitochondrial membrane potential (DeltaPsim) were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots. RESULTS: Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of DeltaPsim, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2. CONCLUSION: The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.
Persistent Identifierhttp://hdl.handle.net/10722/166087
ISSN
2015 Impact Factor: 1.58
2015 SCImago Journal Rankings: 0.655
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSong, JXen_HK
dc.contributor.authorChoi, MYMen_HK
dc.contributor.authorWong, KCKen_HK
dc.contributor.authorChung, WWYen_HK
dc.contributor.authorSze, CWen_HK
dc.contributor.authorNg, TBen_HK
dc.contributor.authorZhang, KYBen_HK
dc.date.accessioned2012-09-20T08:27:58Z-
dc.date.available2012-09-20T08:27:58Z-
dc.date.issued2012en_HK
dc.identifier.citationChinese Medicine, 2012, v. 7 n. 1, article no. 1en_HK
dc.identifier.issn1749-8546en_HK
dc.identifier.urihttp://hdl.handle.net/10722/166087-
dc.description.abstractBACKGROUND: Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism. METHODS: Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS) and loss of mitochondrial membrane potential (DeltaPsim) were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots. RESULTS: Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of DeltaPsim, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2. CONCLUSION: The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/homeen_HK
dc.relation.ispartofChinese Medicineen_HK
dc.rightsChinese Medicine. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectApoptosis-
dc.subjectCell structure-
dc.subjectCell viability-
dc.subjectControlled study-
dc.subjectCytotoxicity-
dc.titleBaicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonismen_HK
dc.typeArticleen_HK
dc.identifier.emailSze, CW: stephens@hku.hken_HK
dc.identifier.emailZhang, KYB: ybzhang@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityZhang, KYB=rp01410en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1749-8546-7-1en_HK
dc.identifier.pmid22264378-
dc.identifier.pmcidPMC3275529-
dc.identifier.scopuseid_2-s2.0-84856011723en_HK
dc.identifier.hkuros202969en_US
dc.identifier.hkuros209479-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84856011723&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue1, article no. 1-
dc.identifier.isiWOS:000320456900001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, KYB=23483121900en_HK
dc.identifier.scopusauthoridNg, TB=35311803300en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridChung, WWY=54960057800en_HK
dc.identifier.scopusauthoridWong, KCK=54961623800en_HK
dc.identifier.scopusauthoridChoi, MYM=54959521400en_HK
dc.identifier.scopusauthoridSong, JX=24339343800en_HK

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