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Article: Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR

TitleMajor human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR
Authors
Yang, NChu, DLHWong, MMLQi, HWu, RSSKong, RYC
KeywordsFecal contamination
HAV genotype IB
Hepatitis A virus
Hong Kong marine waters
TaqMan real-time PCR
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
Citation
Marine Pollution Bulletin, 2011, v. 62 n. 12, p. 2654-2658 How to Cite?
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.09.027
AbstractMarine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/165977
ISSN
0025-326X
2021 Impact Factor: 7.001
2020 SCImago Journal Rankings: 1.548
ISI Accession Number ID
WOS:000298520400023
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorYang, Nen_HK
dc.contributor.authorChu, DLHen_HK
dc.contributor.authorWong, MMLen_HK
dc.contributor.authorQi, Hen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2012-09-20T08:25:57Z-
dc.date.available2012-09-20T08:25:57Z-
dc.date.issued2011en_HK
dc.identifier.citationMarine Pollution Bulletin, 2011, v. 62 n. 12, p. 2654-2658en_HK
dc.identifier.issn0025-326Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/165977-
dc.description.abstractMarine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbulen_HK
dc.relation.ispartofMarine Pollution Bulletinen_HK
dc.subjectFecal contamination-
dc.subjectHAV genotype IB-
dc.subjectHepatitis A virus-
dc.subjectHong Kong marine waters-
dc.subjectTaqMan real-time PCR-
dc.subject.meshEnvironmental Monitoring - methodsen_HK
dc.subject.meshEscherichia coli - isolation & purificationen_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHepatitis A Virus, Human - classification - genetics - isolation & purificationen_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshReal-Time Polymerase Chain Reaction - methodsen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshSeawater - analysisen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSewage - analysisen_HK
dc.subject.meshWater Pollution, Chemical - analysisen_HK
dc.titleMajor human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCRen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.marpolbul.2011.09.027en_HK
dc.identifier.pmid22001296-
dc.identifier.scopuseid_2-s2.0-81855194308en_HK
dc.identifier.hkuros209779en_US
dc.identifier.hkuros203340-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-81855194308&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume62en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2654en_HK
dc.identifier.epage2658en_HK
dc.identifier.isiWOS:000298520400023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, N=54781922300en_HK
dc.identifier.scopusauthoridChu, DLH=25229992700en_HK
dc.identifier.scopusauthoridWong, MML=35390115400en_HK
dc.identifier.scopusauthoridQi, H=54781674400en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK
dc.identifier.citeulike9931429-
dc.identifier.issnl0025-326X-

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