Article: Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR

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TitleMajor human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR
AuthorsYang, N2
Chu, DLH2
Wong, MML2
Qi, H2
Wu, RSS1
Kong, RYC2
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
CitationMarine Pollution Bulletin, 2011, v. 62 n. 12, p. 2654-2658 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.09.027
AbstractMarine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.
ISSN0025-326X
2011 Impact Factor: 2.503
2011 SCImago Journal Rankings: 0.098
DOIhttp://dx.doi.org/10.1016/j.marpolbul.2011.09.027
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorYang, N
dc.contributor.authorChu, DLH
dc.contributor.authorWong, MML
dc.contributor.authorQi, H
dc.contributor.authorWu, RSS
dc.contributor.authorKong, RYC
dc.date.accessioned2012-09-20T08:25:57Z
dc.date.available2012-09-20T08:25:57Z
dc.date.issued2011
dc.description.abstractMarine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMarine Pollution Bulletin, 2011, v. 62 n. 12, p. 2654-2658 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.09.027
dc.identifier.citeulike9931429
dc.identifier.doihttp://dx.doi.org/10.1016/j.marpolbul.2011.09.027
dc.identifier.epage2658
dc.identifier.hkuros209779
dc.identifier.hkuros203340
dc.identifier.issn0025-326X
2011 Impact Factor: 2.503
2011 SCImago Journal Rankings: 0.098
dc.identifier.issue12
dc.identifier.pmid22001296
dc.identifier.scopuseid_2-s2.0-81855194308
dc.identifier.spage2654
dc.identifier.urihttp://hdl.handle.net/10722/165977
dc.identifier.volume62
dc.languageeng
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
dc.publisher.placeUnited Kingdom
dc.relation.ispartofMarine Pollution Bulletin
dc.relation.referencesReferences in Scopus
dc.subject.meshEnvironmental Monitoring - methods
dc.subject.meshEscherichia coli - isolation & purification
dc.subject.meshGenotype
dc.subject.meshHepatitis A Virus, Human - classification - genetics - isolation & purification
dc.subject.meshHong Kong
dc.subject.meshHumans
dc.subject.meshPhylogeny
dc.subject.meshReal-Time Polymerase Chain Reaction - methods
dc.subject.meshReproducibility of Results
dc.subject.meshSeawater - analysis
dc.subject.meshSensitivity and Specificity
dc.subject.meshSequence Analysis, DNA
dc.subject.meshSewage - analysis
dc.subject.meshWater Pollution, Chemical - analysis
dc.titleMajor human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong