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Article: Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR
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TitleMajor human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR
 
AuthorsYang, N2
Chu, DLH2
Wong, MML2
Qi, H2
Wu, RSS1
Kong, RYC2
 
Issue Date2011
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
 
CitationMarine Pollution Bulletin, 2011, v. 62 n. 12, p. 2654-2658 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.09.027
 
AbstractMarine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.
 
ISSN0025-326X
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
 
DOIhttp://dx.doi.org/10.1016/j.marpolbul.2011.09.027
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, N
 
dc.contributor.authorChu, DLH
 
dc.contributor.authorWong, MML
 
dc.contributor.authorQi, H
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorKong, RYC
 
dc.date.accessioned2012-09-20T08:25:57Z
 
dc.date.available2012-09-20T08:25:57Z
 
dc.date.issued2011
 
dc.description.abstractMarine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMarine Pollution Bulletin, 2011, v. 62 n. 12, p. 2654-2658 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.09.027
 
dc.identifier.citeulike9931429
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.marpolbul.2011.09.027
 
dc.identifier.epage2658
 
dc.identifier.hkuros209779
 
dc.identifier.hkuros203340
 
dc.identifier.issn0025-326X
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
 
dc.identifier.issue12
 
dc.identifier.pmid22001296
 
dc.identifier.scopuseid_2-s2.0-81855194308
 
dc.identifier.spage2654
 
dc.identifier.urihttp://hdl.handle.net/10722/165977
 
dc.identifier.volume62
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofMarine Pollution Bulletin
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshEnvironmental Monitoring - methods
 
dc.subject.meshEscherichia coli - isolation & purification
 
dc.subject.meshGenotype
 
dc.subject.meshHepatitis A Virus, Human - classification - genetics - isolation & purification
 
dc.subject.meshHong Kong
 
dc.subject.meshHumans
 
dc.subject.meshPhylogeny
 
dc.subject.meshReal-Time Polymerase Chain Reaction - methods
 
dc.subject.meshReproducibility of Results
 
dc.subject.meshSeawater - analysis
 
dc.subject.meshSensitivity and Specificity
 
dc.subject.meshSequence Analysis, DNA
 
dc.subject.meshSewage - analysis
 
dc.subject.meshWater Pollution, Chemical - analysis
 
dc.titleMajor human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR
 
dc.typeArticle
 
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<contributor.author>Kong, RYC</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong