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Article: High incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong
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TitleHigh incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong
 
AuthorsZhang, R2 1
Pan, L2
Zhao, Z2 3
Gu, JD2
 
KeywordsVibrio cholerae
Replication
Plasmid
Mai Po Nature Reserve
Diversity
Distribution
 
Issue Date2012
 
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
 
CitationEcotoxicology, 2012, v. 21 n. 6, p. 1661-1668 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10646-012-0939-7
 
AbstractMai Po Nature Reserve is the largest mangrove ecosystem and the most polluted coastal water body in Hong Kong. Plasmids screening of 100 Vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. These plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. Phylogenetic analysis of the partial 16S rRNA gene sequences confirmed that all plasmid(s)-bearing isolates belonged to Vibrio cholerae. Full DNA sequences of the plasmids in Groups I (pVCG1.1 and pVCG1.2), II (pVCG2.1), III (pVCG3.2) and IV (pVCG4.1) have been determined and the results showed that pVCG1.1, pVCG2.1 and pVCG3.2 were almost identical. Plasmids pVCG1.1, pVCG1.2 and pVCG4.1 are comprised of 4,439, 2,357 and 2,163 bp with the overall G+C content of 45.57, 53.54 and 43.09 %, respectively. pVCG1.1 is a novel plasmid, and plasmids pVCG1.2 and pVCG4.1 showed homology of replication initiation proteins to that of the theta type replicons. Attempts to cure the plasmids from their hosts were unsuccessful. These data suggest that plasmids of Vibrio spp. are a significant gene reservoir in the marine ecosystem.
 
DescriptionSpringer open access article
 
ISSN0963-9292
2012 Impact Factor: 2.773
2012 SCImago Journal Rankings: 1.041
 
DOIhttp://dx.doi.org/10.1007/s10646-012-0939-7
 
PubMed Central IDPMC3399079
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhang, R
 
dc.contributor.authorPan, L
 
dc.contributor.authorZhao, Z
 
dc.contributor.authorGu, JD
 
dc.date.accessioned2012-09-20T08:25:55Z
 
dc.date.available2012-09-20T08:25:55Z
 
dc.date.issued2012
 
dc.description.abstractMai Po Nature Reserve is the largest mangrove ecosystem and the most polluted coastal water body in Hong Kong. Plasmids screening of 100 Vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. These plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. Phylogenetic analysis of the partial 16S rRNA gene sequences confirmed that all plasmid(s)-bearing isolates belonged to Vibrio cholerae. Full DNA sequences of the plasmids in Groups I (pVCG1.1 and pVCG1.2), II (pVCG2.1), III (pVCG3.2) and IV (pVCG4.1) have been determined and the results showed that pVCG1.1, pVCG2.1 and pVCG3.2 were almost identical. Plasmids pVCG1.1, pVCG1.2 and pVCG4.1 are comprised of 4,439, 2,357 and 2,163 bp with the overall G+C content of 45.57, 53.54 and 43.09 %, respectively. pVCG1.1 is a novel plasmid, and plasmids pVCG1.2 and pVCG4.1 showed homology of replication initiation proteins to that of the theta type replicons. Attempts to cure the plasmids from their hosts were unsuccessful. These data suggest that plasmids of Vibrio spp. are a significant gene reservoir in the marine ecosystem.
 
dc.description.naturepublished_or_final_version
 
dc.descriptionSpringer open access article
 
dc.identifier.citationEcotoxicology, 2012, v. 21 n. 6, p. 1661-1668 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10646-012-0939-7
 
dc.identifier.citeulike10783166
 
dc.identifier.doihttp://dx.doi.org/10.1007/s10646-012-0939-7
 
dc.identifier.epage1668
 
dc.identifier.hkuros209712
 
dc.identifier.hkuros224960
 
dc.identifier.issn0963-9292
2012 Impact Factor: 2.773
2012 SCImago Journal Rankings: 1.041
 
dc.identifier.issue6
 
dc.identifier.pmcidPMC3399079
 
dc.identifier.pmid22684730
 
dc.identifier.scopuseid_2-s2.0-84864871908
 
dc.identifier.spage1661
 
dc.identifier.urihttp://hdl.handle.net/10722/165970
 
dc.identifier.volume21
 
dc.languageeng
 
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
 
dc.publisher.placeUnited States
 
dc.relation.ispartofEcotoxicology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAnti-Bacterial Agents - metabolism
 
dc.subject.meshBiodiversity
 
dc.subject.meshPlasmids - genetics
 
dc.subject.meshVibrio cholerae - classification - genetics - growth and development - isolation and purification
 
dc.subject.meshWater Microbiology
 
dc.subjectVibrio cholerae
 
dc.subjectReplication
 
dc.subjectPlasmid
 
dc.subjectMai Po Nature Reserve
 
dc.subjectDiversity
 
dc.subjectDistribution
 
dc.titleHigh incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong
 
dc.typeArticle
 
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Author Affiliations
  1. Nanjing Agricultural University
  2. The University of Hong Kong
  3. Research Environment Engineering Technique Co., Ltd.