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Article: High incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong

TitleHigh incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong
Authors
KeywordsVibrio cholerae
Replication
Plasmid
Mai Po Nature Reserve
Diversity
Distribution
Issue Date2012
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
Citation
Ecotoxicology, 2012, v. 21 n. 6, p. 1661-1668 How to Cite?
AbstractMai Po Nature Reserve is the largest mangrove ecosystem and the most polluted coastal water body in Hong Kong. Plasmids screening of 100 Vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. These plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. Phylogenetic analysis of the partial 16S rRNA gene sequences confirmed that all plasmid(s)-bearing isolates belonged to Vibrio cholerae. Full DNA sequences of the plasmids in Groups I (pVCG1.1 and pVCG1.2), II (pVCG2.1), III (pVCG3.2) and IV (pVCG4.1) have been determined and the results showed that pVCG1.1, pVCG2.1 and pVCG3.2 were almost identical. Plasmids pVCG1.1, pVCG1.2 and pVCG4.1 are comprised of 4,439, 2,357 and 2,163 bp with the overall G+C content of 45.57, 53.54 and 43.09 %, respectively. pVCG1.1 is a novel plasmid, and plasmids pVCG1.2 and pVCG4.1 showed homology of replication initiation proteins to that of the theta type replicons. Attempts to cure the plasmids from their hosts were unsuccessful. These data suggest that plasmids of Vibrio spp. are a significant gene reservoir in the marine ecosystem.
Persistent Identifierhttp://hdl.handle.net/10722/165970
ISSN
2014 Impact Factor: 2.706
2014 SCImago Journal Rankings: 1.080
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ren_HK
dc.contributor.authorPan, Len_HK
dc.contributor.authorZhao, Zen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2012-09-20T08:25:55Z-
dc.date.available2012-09-20T08:25:55Z-
dc.date.issued2012en_HK
dc.identifier.citationEcotoxicology, 2012, v. 21 n. 6, p. 1661-1668en_HK
dc.identifier.issn0963-9292en_HK
dc.identifier.urihttp://hdl.handle.net/10722/165970-
dc.description.abstractMai Po Nature Reserve is the largest mangrove ecosystem and the most polluted coastal water body in Hong Kong. Plasmids screening of 100 Vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. These plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. Phylogenetic analysis of the partial 16S rRNA gene sequences confirmed that all plasmid(s)-bearing isolates belonged to Vibrio cholerae. Full DNA sequences of the plasmids in Groups I (pVCG1.1 and pVCG1.2), II (pVCG2.1), III (pVCG3.2) and IV (pVCG4.1) have been determined and the results showed that pVCG1.1, pVCG2.1 and pVCG3.2 were almost identical. Plasmids pVCG1.1, pVCG1.2 and pVCG4.1 are comprised of 4,439, 2,357 and 2,163 bp with the overall G+C content of 45.57, 53.54 and 43.09 %, respectively. pVCG1.1 is a novel plasmid, and plasmids pVCG1.2 and pVCG4.1 showed homology of replication initiation proteins to that of the theta type replicons. Attempts to cure the plasmids from their hosts were unsuccessful. These data suggest that plasmids of Vibrio spp. are a significant gene reservoir in the marine ecosystem.en_HK
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292en_HK
dc.relation.ispartofEcotoxicologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectVibrio choleraeen_HK
dc.subjectReplicationen_HK
dc.subjectPlasmiden_HK
dc.subjectMai Po Nature Reserveen_HK
dc.subjectDiversityen_HK
dc.subjectDistributionen_HK
dc.subject.meshAnti-Bacterial Agents - metabolism-
dc.subject.meshBiodiversity-
dc.subject.meshPlasmids - genetics-
dc.subject.meshVibrio cholerae - classification - genetics - growth and development - isolation and purification-
dc.subject.meshWater Microbiology-
dc.titleHigh incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.emailZhang, R: rfzhang@njau.edu.cnen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hk-
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1007/s10646-012-0939-7en_HK
dc.identifier.pmid22684730-
dc.identifier.pmcidPMC3399079-
dc.identifier.scopuseid_2-s2.0-84864871908en_HK
dc.identifier.hkuros209712en_US
dc.identifier.hkuros224960-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84864871908&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1661en_HK
dc.identifier.epage1668en_HK
dc.identifier.isiWOS:000306592100011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridZhao, Z=7404148369en_HK
dc.identifier.scopusauthoridPan, L=54393873900en_HK
dc.identifier.scopusauthoridZhang, R=37121280100en_HK
dc.identifier.citeulike10783166-

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