Article: High incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong

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Publisher Website: 10.1007/s10646-012-0939-7
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Title	High incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong
Authors	Zhang, R1 Pan, L1 Zhao, Z1 Gu, JD1
Keywords	Vibrio cholerae Replication Plasmid Mai Po Nature Reserve Diversity Distribution
Issue Date	2012
Publisher	Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
Citation	Ecotoxicology, 2012, v. 21 n. 6, p. 1661-1668 [How to Cite?] DOI: http://dx.doi.org/10.1007/s10646-012-0939-7
Abstract	Mai Po Nature Reserve is the largest mangrove ecosystem and the most polluted coastal water body in Hong Kong. Plasmids screening of 100 Vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. These plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. Phylogenetic analysis of the partial 16S rRNA gene sequences confirmed that all plasmid(s)-bearing isolates belonged to Vibrio cholerae. Full DNA sequences of the plasmids in Groups I (pVCG1.1 and pVCG1.2), II (pVCG2.1), III (pVCG3.2) and IV (pVCG4.1) have been determined and the results showed that pVCG1.1, pVCG2.1 and pVCG3.2 were almost identical. Plasmids pVCG1.1, pVCG1.2 and pVCG4.1 are comprised of 4,439, 2,357 and 2,163 bp with the overall G+C content of 45.57, 53.54 and 43.09 %, respectively. pVCG1.1 is a novel plasmid, and plasmids pVCG1.2 and pVCG4.1 showed homology of replication initiation proteins to that of the theta type replicons. Attempts to cure the plasmids from their hosts were unsuccessful. These data suggest that plasmids of Vibrio spp. are a significant gene reservoir in the marine ecosystem.
Description	Springer open access article
ISSN	0963-9292 2011 Impact Factor: 2.355 2011 SCImago Journal Rankings: 0.141
DOI	http://dx.doi.org/10.1007/s10646-012-0939-7
PubMed Central ID	PMC3399079
References	References in Scopus

DC Field	Value
dc.contributor.author	Zhang, R
dc.contributor.author	Pan, L
dc.contributor.author	Zhao, Z
dc.contributor.author	Gu, JD
dc.date.accessioned	2012-09-20T08:25:55Z
dc.date.available	2012-09-20T08:25:55Z
dc.date.issued	2012
dc.description.abstract	Mai Po Nature Reserve is the largest mangrove ecosystem and the most polluted coastal water body in Hong Kong. Plasmids screening of 100 Vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. These plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. Phylogenetic analysis of the partial 16S rRNA gene sequences confirmed that all plasmid(s)-bearing isolates belonged to Vibrio cholerae. Full DNA sequences of the plasmids in Groups I (pVCG1.1 and pVCG1.2), II (pVCG2.1), III (pVCG3.2) and IV (pVCG4.1) have been determined and the results showed that pVCG1.1, pVCG2.1 and pVCG3.2 were almost identical. Plasmids pVCG1.1, pVCG1.2 and pVCG4.1 are comprised of 4,439, 2,357 and 2,163 bp with the overall G+C content of 45.57, 53.54 and 43.09 %, respectively. pVCG1.1 is a novel plasmid, and plasmids pVCG1.2 and pVCG4.1 showed homology of replication initiation proteins to that of the theta type replicons. Attempts to cure the plasmids from their hosts were unsuccessful. These data suggest that plasmids of Vibrio spp. are a significant gene reservoir in the marine ecosystem.
dc.description.nature	published_or_final_version
dc.description	Springer open access article
dc.identifier.citation	Ecotoxicology, 2012, v. 21 n. 6, p. 1661-1668 [How to Cite?] DOI: http://dx.doi.org/10.1007/s10646-012-0939-7
dc.identifier.citeulike	10783166
dc.identifier.doi	http://dx.doi.org/10.1007/s10646-012-0939-7
dc.identifier.epage	1668
dc.identifier.hkuros	209712
dc.identifier.issn	0963-9292 2011 Impact Factor: 2.355 2011 SCImago Journal Rankings: 0.141
dc.identifier.issue	6
dc.identifier.pmcid	PMC3399079
dc.identifier.pmid	22684730
dc.identifier.scopus	eid_2-s2.0-84864871908
dc.identifier.spage	1661
dc.identifier.uri	http://hdl.handle.net/10722/165970
dc.identifier.volume	21
dc.language	eng
dc.publisher	Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
dc.publisher.place	United States
dc.relation.ispartof	Ecotoxicology
dc.relation.references	References in Scopus
dc.rights	Creative Commons: Attribution 3.0 Hong Kong License
dc.subject.mesh	Anti-Bacterial Agents - metabolism
dc.subject.mesh	Biodiversity
dc.subject.mesh	Plasmids - genetics
dc.subject.mesh	Vibrio cholerae - classification - genetics - growth and development - isolation and purification
dc.subject.mesh	Water Microbiology
dc.subject	Vibrio cholerae
dc.subject	Replication
dc.subject	Plasmid
dc.subject	Mai Po Nature Reserve
dc.subject	Diversity
dc.subject	Distribution
dc.title	High incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong
dc.type	Article

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Author Affiliations

The University of Hong Kong