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Article: Microbial community characteristics of petroleum reservoir production water amended with n-alkanes and incubated under nitrate-, sulfate-reducing and methanogenic conditions

TitleMicrobial community characteristics of petroleum reservoir production water amended with n-alkanes and incubated under nitrate-, sulfate-reducing and methanogenic conditions
Authors
Keywords16s rrna gene
Actinobacteria
Archaeal
Clone library
Crenarchaeota
Issue Date2012
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
Citation
International Biodeterioration and Biodegradation, 2012, v. 69, p. 87-96 How to Cite?
Abstract
Methanogenic, sulfate- and nitrate-reducing enrichment cultures amended with long-chain n-alkanes (C 15-C 20) were established with production water from Huabei oilfield in China in the present study. Chemical analyses indicated that degradation of n-alkanes was evident under all three conditions after 356 days of incubation. Phylogenetic analyses based on 16S rRNA gene amplification indicated that α-, β-, γ-Proteobacteria and Bacteroidetes were detected in the nitrate-reducing enrichment; Actinobacteria, Nitrospira and δ-Proteobacteria were recovered from both the sulfate-reducing and methanogenic enrichments. Actinobacteria and Nitrospira were the most abundant in methanogenic and sulfate-reducing enrichment, respectively. The archaeal clone libraries showed that the order Methanomicrobiales within the phylum Euryarchaeota predominated methanogenic enrichment; whereas the unclassified class Thermoprotei within the phylum Crenarchaeota prevailed in sulfate-reducing enrichment. Comparison of 16S rRNA gene sequences from genomic DNA extracted directly from the petroleum reservoir production water with those from the three active enrichments showed that the available electron acceptors had a strong influence on the microbial community composition. In addition, genes encoding the alkylsuccinate synthase (assA) and methyl coenzyme-M reductase (mcrA) were amplified from the methanogenic enrichment and the results suggested that fumarate addition was probably involved in the degradation of n-alkanes. These results shed light on the potential utilization of microbial metabolism in remediation of hydrocarbon contamination or in enhancing the recovery of residual oil for energy. © 2012 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/165966
ISSN
2013 Impact Factor: 2.235
2013 SCImago Journal Rankings: 0.884
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Wen_HK
dc.contributor.authorWang, LYen_HK
dc.contributor.authorDuan, RYen_HK
dc.contributor.authorLiu, JFen_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorMu, BZen_HK
dc.date.accessioned2012-09-20T08:25:53Z-
dc.date.available2012-09-20T08:25:53Z-
dc.date.issued2012en_HK
dc.identifier.citationInternational Biodeterioration and Biodegradation, 2012, v. 69, p. 87-96en_HK
dc.identifier.issn0964-8305en_HK
dc.identifier.urihttp://hdl.handle.net/10722/165966-
dc.description.abstractMethanogenic, sulfate- and nitrate-reducing enrichment cultures amended with long-chain n-alkanes (C 15-C 20) were established with production water from Huabei oilfield in China in the present study. Chemical analyses indicated that degradation of n-alkanes was evident under all three conditions after 356 days of incubation. Phylogenetic analyses based on 16S rRNA gene amplification indicated that α-, β-, γ-Proteobacteria and Bacteroidetes were detected in the nitrate-reducing enrichment; Actinobacteria, Nitrospira and δ-Proteobacteria were recovered from both the sulfate-reducing and methanogenic enrichments. Actinobacteria and Nitrospira were the most abundant in methanogenic and sulfate-reducing enrichment, respectively. The archaeal clone libraries showed that the order Methanomicrobiales within the phylum Euryarchaeota predominated methanogenic enrichment; whereas the unclassified class Thermoprotei within the phylum Crenarchaeota prevailed in sulfate-reducing enrichment. Comparison of 16S rRNA gene sequences from genomic DNA extracted directly from the petroleum reservoir production water with those from the three active enrichments showed that the available electron acceptors had a strong influence on the microbial community composition. In addition, genes encoding the alkylsuccinate synthase (assA) and methyl coenzyme-M reductase (mcrA) were amplified from the methanogenic enrichment and the results suggested that fumarate addition was probably involved in the degradation of n-alkanes. These results shed light on the potential utilization of microbial metabolism in remediation of hydrocarbon contamination or in enhancing the recovery of residual oil for energy. © 2012 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibioden_HK
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_HK
dc.subject16s rrna geneen_HK
dc.subjectActinobacteriaen_HK
dc.subjectArchaealen_HK
dc.subjectClone libraryen_HK
dc.subjectCrenarchaeota-
dc.titleMicrobial community characteristics of petroleum reservoir production water amended with n-alkanes and incubated under nitrate-, sulfate-reducing and methanogenic conditionsen_HK
dc.typeArticleen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.emailMu, BZ: bzmu@ecust.edu.cn-
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ibiod.2012.01.005en_HK
dc.identifier.scopuseid_2-s2.0-84862829149en_HK
dc.identifier.hkuros209631en_US
dc.identifier.hkuros224966-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862829149&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume69en_HK
dc.identifier.spage87en_HK
dc.identifier.epage96en_HK
dc.identifier.isiWOS:000302051500013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMu, BZ=7004585949en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridLiu, JF=36096880500en_HK
dc.identifier.scopusauthoridDuan, RY=55260893500en_HK
dc.identifier.scopusauthoridWang, LY=36803082300en_HK
dc.identifier.scopusauthoridLi, W=55120957300en_HK
dc.identifier.citeulike10382687-

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