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Article: Filamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis

TitleFilamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis
Authors
KeywordsFilamin A
Small interfering RNA
Actin filament
Blood testis barrier
Cytoskeleton
Issue Date2012
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2012, v. 153 n. 10, p. 5023-5035 How to Cite?
AbstractThe blood-testis barrier (BTB) is an important ultrastructure in the testis. A delay in its assembly during postnatal development leads to meiotic arrest. Also, a disruption of the BTB by toxicants in adult rats leads to a failure in spermatogonial differentiation. However, the regulation of BTB assembly remains unknown. Herein, filamin A, an actin filament cross-linker that is known to maintain and regulate cytoskeleton structure and function in other epithelia, was shown to be highly expressed during the assembly of Sertoli cell BTB in vitro and postnatal development of BTB in vivo, perhaps being used to maintain the actin filament network at the BTB. A knockdown of filamin A by RNA interference was found to partially perturb the Sertoli cell tight junction (TJ) permeability barrier both in vitro and in vivo. Interestingly, this down-regulating effect on the TJ barrier function after the knockdown of filamin A was associated with a mis-localization of both TJ and basal ectoplasmic specialization proteins. Filamin A knockdown also induced a disorganization of the actin filament network in Sertoli cells in vitro and in vivo. Collectively, these findings illustrate that filamin A regulates BTB assembly by recruiting these proteins to the microenvironment in the seminiferous epithelium to serve as the building blocks. In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis. Copyright © 2012 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/165938
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSu, Wen_HK
dc.contributor.authorMruk, DDen_HK
dc.contributor.authorLie, PPYen_HK
dc.contributor.authorLui, WYen_HK
dc.contributor.authorCheng, CYen_HK
dc.date.accessioned2012-09-20T08:25:41Z-
dc.date.available2012-09-20T08:25:41Z-
dc.date.issued2012en_HK
dc.identifier.citationEndocrinology, 2012, v. 153 n. 10, p. 5023-5035en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/165938-
dc.description.abstractThe blood-testis barrier (BTB) is an important ultrastructure in the testis. A delay in its assembly during postnatal development leads to meiotic arrest. Also, a disruption of the BTB by toxicants in adult rats leads to a failure in spermatogonial differentiation. However, the regulation of BTB assembly remains unknown. Herein, filamin A, an actin filament cross-linker that is known to maintain and regulate cytoskeleton structure and function in other epithelia, was shown to be highly expressed during the assembly of Sertoli cell BTB in vitro and postnatal development of BTB in vivo, perhaps being used to maintain the actin filament network at the BTB. A knockdown of filamin A by RNA interference was found to partially perturb the Sertoli cell tight junction (TJ) permeability barrier both in vitro and in vivo. Interestingly, this down-regulating effect on the TJ barrier function after the knockdown of filamin A was associated with a mis-localization of both TJ and basal ectoplasmic specialization proteins. Filamin A knockdown also induced a disorganization of the actin filament network in Sertoli cells in vitro and in vivo. Collectively, these findings illustrate that filamin A regulates BTB assembly by recruiting these proteins to the microenvironment in the seminiferous epithelium to serve as the building blocks. In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis. Copyright © 2012 by The Endocrine Society.en_HK
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.subjectFilamin A-
dc.subjectSmall interfering RNA-
dc.subjectActin filament-
dc.subjectBlood testis barrier-
dc.subjectCytoskeleton-
dc.titleFilamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testisen_HK
dc.typeArticleen_HK
dc.identifier.emailLui, WY: wylui@hku.hken_HK
dc.identifier.authorityLui, WY=rp00756en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2012-1286en_HK
dc.identifier.pmid22872576-
dc.identifier.scopuseid_2-s2.0-84866753015en_HK
dc.identifier.hkuros206215en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84866753015&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume153en_HK
dc.identifier.issue10en_HK
dc.identifier.spage5023en_HK
dc.identifier.epage5035en_HK
dc.identifier.isiWOS:000309210200043-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSu, W=7402009940en_HK
dc.identifier.scopusauthoridMruk, DD=6701823934en_HK
dc.identifier.scopusauthoridLie, PPY=15839862700en_HK
dc.identifier.scopusauthoridLui, WY=35220192400en_HK
dc.identifier.scopusauthoridCheng, CY=7404797787en_HK

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