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Conference Paper: A systematic re-evaluation of enzymatic isolation methodology for nucleus pulposus cells

TitleA systematic re-evaluation of enzymatic isolation methodology for nucleus pulposus cells
Authors
Issue Date2012
PublisherGeorg Thieme Verlag. The Journal's web site is located at http://www.thieme.com/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=1351&category_id=90&option=com_virtuemart&Itemid=53
Citation
The World Forum for Spine Research (WFSR 2012): The Intervertebral Disc - from Degeneration to Pain, Helsinki, Finland, 18-21 June 2012. In Global Spine Journal, 2012, v. 2 n. S1, no. P55 How to Cite?
AbstractINTRODUCTION: The nucleus pulposus (NP) is the central part of IVD and understanding its function is essential for studying IVD degeneration. Enzymatic digestion by collagenase is used to release cells from NP tissues for cell phenotype characterization or further in vitro cell culture studies. For cell isolation from NP of different species, various protocols exist and vary greatly in terms of enzyme concentration, digestion duration, and type of precollagenase treatments such as digestion by pronase, which is a mixture of different proteases. In contrast to NP from other species, the cells in bovine or human mature NP have fewer cell-cell contacts and are surrounded by less gel-like matrix rich in proteoglycan and collagen. Thus we hypothesized that the pronase dig...
Persistent Identifierhttp://hdl.handle.net/10722/165553
ISSN
2015 SCImago Journal Rankings: 0.108

 

DC FieldValueLanguage
dc.contributor.authorLee, JTYen_US
dc.contributor.authorLeung, VYLen_US
dc.contributor.authorTam, Ven_US
dc.contributor.authorCheung, KMCen_US
dc.date.accessioned2012-09-20T08:19:30Z-
dc.date.available2012-09-20T08:19:30Z-
dc.date.issued2012en_US
dc.identifier.citationThe World Forum for Spine Research (WFSR 2012): The Intervertebral Disc - from Degeneration to Pain, Helsinki, Finland, 18-21 June 2012. In Global Spine Journal, 2012, v. 2 n. S1, no. P55en_US
dc.identifier.issn2192-5682-
dc.identifier.urihttp://hdl.handle.net/10722/165553-
dc.description.abstractINTRODUCTION: The nucleus pulposus (NP) is the central part of IVD and understanding its function is essential for studying IVD degeneration. Enzymatic digestion by collagenase is used to release cells from NP tissues for cell phenotype characterization or further in vitro cell culture studies. For cell isolation from NP of different species, various protocols exist and vary greatly in terms of enzyme concentration, digestion duration, and type of precollagenase treatments such as digestion by pronase, which is a mixture of different proteases. In contrast to NP from other species, the cells in bovine or human mature NP have fewer cell-cell contacts and are surrounded by less gel-like matrix rich in proteoglycan and collagen. Thus we hypothesized that the pronase dig...-
dc.languageengen_US
dc.publisherGeorg Thieme Verlag. The Journal's web site is located at http://www.thieme.com/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=1351&category_id=90&option=com_virtuemart&Itemid=53-
dc.relation.ispartofGlobal Spine Journalen_US
dc.rightsGlobal Spine Journal. Copyright © Georg Thieme Verlag.-
dc.titleA systematic re-evaluation of enzymatic isolation methodology for nucleus pulposus cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLee, JTY: juliana.lee@hku.hken_US
dc.identifier.emailLeung, VYL: vicleung@hku.hken_US
dc.identifier.emailTam, V: vivtam@hku.hken_US
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_US
dc.identifier.authorityCheung, KMC=rp00387en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1055/s-0032-1319932-
dc.identifier.hkuros210485en_US
dc.identifier.volume2-
dc.identifier.issueS1-
dc.publisher.placeGermany-

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