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Conference Paper: Immobilization of chondroitinase ABCI on chitosan beads to improve axonal regrowth in CSPG-enriched astrocyte culture

TitleImmobilization of chondroitinase ABCI on chitosan beads to improve axonal regrowth in CSPG-enriched astrocyte culture
Authors
Issue Date2012
PublisherHKSN & BPHK.
Citation
The Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 75, abstract P62 How to Cite?
AbstractAfter nerve injury, scar tissue enriched in chondroitin sulfate proteoglycans (CSPGs) is often formed to contain the lesion but is restrictive to axonal regrowth. Chondroitinase ABCI (ChABCI) has been exploited to cleave CS moieties of the PGs and thus to enhance prospects of axonal regrowth through the lesion. ChABCI activity decay in vivo has however hindered application of the enzyme in nerve regeneration. We attempted to address this by immobilization of recombinant ChABCI on a selected matrix. Chitosan was chosen because it is non-toxic, biocompatible and tunable in rate of biodegradation. We prepared chitosan beads (73.89μm±28.59) that precluded phagocytosis by inflammatory cells. ChABCI that was cross-linked to the chitosan beads under specified conditions demonstrated CS-cleaving activity both in biochemical assay and in astrocyte cultures that had been activated to secrete CSPGs. Factors that contribute to activity decay as thermal instability and susceptibility to end product inhibition are assessed. Axonal regrowth on activated astrocyte cultures was improved by co-treatment with ChABC I and ChABC II.
DescriptionPoster Presentation: P62
Persistent Identifierhttp://hdl.handle.net/10722/165084

 

DC FieldValueLanguage
dc.contributor.authorKwok, LFen_US
dc.contributor.authorTam, KWen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2012-09-20T08:14:40Z-
dc.date.available2012-09-20T08:14:40Z-
dc.date.issued2012en_US
dc.identifier.citationThe Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 75, abstract P62en_US
dc.identifier.urihttp://hdl.handle.net/10722/165084-
dc.descriptionPoster Presentation: P62-
dc.description.abstractAfter nerve injury, scar tissue enriched in chondroitin sulfate proteoglycans (CSPGs) is often formed to contain the lesion but is restrictive to axonal regrowth. Chondroitinase ABCI (ChABCI) has been exploited to cleave CS moieties of the PGs and thus to enhance prospects of axonal regrowth through the lesion. ChABCI activity decay in vivo has however hindered application of the enzyme in nerve regeneration. We attempted to address this by immobilization of recombinant ChABCI on a selected matrix. Chitosan was chosen because it is non-toxic, biocompatible and tunable in rate of biodegradation. We prepared chitosan beads (73.89μm±28.59) that precluded phagocytosis by inflammatory cells. ChABCI that was cross-linked to the chitosan beads under specified conditions demonstrated CS-cleaving activity both in biochemical assay and in astrocyte cultures that had been activated to secrete CSPGs. Factors that contribute to activity decay as thermal instability and susceptibility to end product inhibition are assessed. Axonal regrowth on activated astrocyte cultures was improved by co-treatment with ChABC I and ChABC II.-
dc.languageengen_US
dc.publisherHKSN & BPHK.-
dc.relation.ispartofProgram Book of the Joint Scientific Meetingen_US
dc.titleImmobilization of chondroitinase ABCI on chitosan beads to improve axonal regrowth in CSPG-enriched astrocyte cultureen_US
dc.typeConference_Paperen_US
dc.identifier.emailKwok, LF: lamfungs@hku.hken_US
dc.identifier.emailTam, KW: tamkw@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros200592en_US
dc.identifier.hkuros209441-
dc.identifier.spage75-
dc.identifier.epage75-
dc.publisher.placeHong Kong-

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