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Conference Paper: Signal involved in the switch to fate commitment of bone marrow-derived Schwann cells

TitleSignal involved in the switch to fate commitment of bone marrow-derived Schwann cells
Authors
Issue Date2012
PublisherHKSN & BPHK.
Citation
The Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 74-75, abstract P61 How to Cite?
AbstractSchwann cell transplantation can promote axonal regrowth and remyelination following nerve injury. Our group has reported the generation of fate-committed bone marrow-derived Schwann cells by co-culturing with dorsal root ganglia (DRG, E14/15 rats) neurons (Shea et. al, 2010). Juxtacrine/paracrine signals from DRG neurons are therefore thought to provide instructive cues to direct the switch of bone marrow-derived Schwann cell-like cells (SCLC) to fate committed Schwann cells. We hypothesized that neuregulin 1 Type III (Nrg 1 Type III), the membrane bound form of Nrg contributes to the switch but not the soluble isoforms. SCLC treated with soluble Nrg did not show significant changes in morphology nor marker expression when compared with untreated SCLCs. Purified DRG neurons were found to express Nrg 1 Type III as indicated by immunocytochemistry and polymerase chain reaction for the mRNA. A Nrg 1 Type III construct was also made for transfection into HEK 293T and mouse embryonic fibroblasts (MEF) such that these could be tested as surrogate cell types in co-culture with SCLCs to pursue cell-specific effects of Nrg 1 Type III on differentiation of SCLCs. The findings promise a way for generating fate committed Schwann cells for autologous transplantation into nerve lesion sites to promote functional recovery.
DescriptionPoster Presentation: P61
Persistent Identifierhttp://hdl.handle.net/10722/165083

 

DC FieldValueLanguage
dc.contributor.authorLeung, KHYen_US
dc.contributor.authorTsui, YPen_US
dc.contributor.authorShea, GKHen_US
dc.contributor.authorTai, EWYen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2012-09-20T08:14:40Z-
dc.date.available2012-09-20T08:14:40Z-
dc.date.issued2012en_US
dc.identifier.citationThe Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 74-75, abstract P61en_US
dc.identifier.urihttp://hdl.handle.net/10722/165083-
dc.descriptionPoster Presentation: P61-
dc.description.abstractSchwann cell transplantation can promote axonal regrowth and remyelination following nerve injury. Our group has reported the generation of fate-committed bone marrow-derived Schwann cells by co-culturing with dorsal root ganglia (DRG, E14/15 rats) neurons (Shea et. al, 2010). Juxtacrine/paracrine signals from DRG neurons are therefore thought to provide instructive cues to direct the switch of bone marrow-derived Schwann cell-like cells (SCLC) to fate committed Schwann cells. We hypothesized that neuregulin 1 Type III (Nrg 1 Type III), the membrane bound form of Nrg contributes to the switch but not the soluble isoforms. SCLC treated with soluble Nrg did not show significant changes in morphology nor marker expression when compared with untreated SCLCs. Purified DRG neurons were found to express Nrg 1 Type III as indicated by immunocytochemistry and polymerase chain reaction for the mRNA. A Nrg 1 Type III construct was also made for transfection into HEK 293T and mouse embryonic fibroblasts (MEF) such that these could be tested as surrogate cell types in co-culture with SCLCs to pursue cell-specific effects of Nrg 1 Type III on differentiation of SCLCs. The findings promise a way for generating fate committed Schwann cells for autologous transplantation into nerve lesion sites to promote functional recovery.-
dc.languageengen_US
dc.publisherHKSN & BPHK.-
dc.relation.ispartofProgram Book of the Joint Scientific Meetingen_US
dc.titleSignal involved in the switch to fate commitment of bone marrow-derived Schwann cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, KHY: kath76@hku.hken_US
dc.identifier.emailTsui, YP: h0694071@graduate.hku.hken_US
dc.identifier.emailShea, GKH: grahams@hku.hken_US
dc.identifier.emailTai, EWY: evietai@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros209438en_US
dc.identifier.spage74-
dc.identifier.epage75-
dc.publisher.placeHong Kong-

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