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Article: The role of cryopreservation in the biomechanical properties of the intervertebral disc

TitleThe role of cryopreservation in the biomechanical properties of the intervertebral disc
Authors
KeywordsAllograft
Biomechanics
Cryopreservation
Intervertebral disc
Transplantation
Issue Date2011
PublisherSwiss Society for Biomaterials. The Journal's web site is located at http://www.ecmjournal.org
Citation
European Cells And Materials, 2011, v. 22, p. 393-402 How to Cite?
AbstractImplantation of intervertebral disc (IVD) allograft or tissue engineered disc constructs in the spine has emerged as an alternative to artificial disc replacement for the treatment of severe degenerative disc disease (DDD). Establishment of a bank of cryopreserved IVD allografts enables size matching and facilitates logistics for effective clinical management. However, the biomechanical properties of cryopreserved IVDs have not been previously reported. This study aimed to assess if cryopreservation with different concentrations of cryopreservant agents (CPA) would affect the dynamic viscoelastic properties of the IVD. Whole porcine lumbar IVDs (n = 40) were harvested and processed using various concentrations of CPA, 0% CPA, 10% CPA and 20% CPA. The discs were cryopreserved using a stepwise freezing protocol and stored in liquid nitrogen. After four weeks of storage, the cryopreserved IVDs were quickly thawed at 37 °C for dynamic viscoelastic testing. The apparent modulus, elastic modulus (G"), viscous modulus (G") and loss modulus (G"/G') were calculated and compared to a fresh control group. Cryopreserved IVD without cryopreservants was significantly stiffer than the control. In the dynamic viscoelastic testing, cryopreservation with the use of CPA was able to preserve both G' and G" of an IVD. No significant differences were found between fresh IVD and IVD cryopreserved with 10% CPA or 20% CPA. This study demonstrated that CPAs at an optimal concentration could preserve the mechanical properties of the IVD allograft and can provide further credence for the application of long-term storage of IVD allografts for disc transplantation or tissue engineered construct applications.
Persistent Identifierhttp://hdl.handle.net/10722/164430
ISSN
2015 Impact Factor: 3.654
2015 SCImago Journal Rankings: 1.771
References

 

DC FieldValueLanguage
dc.contributor.authorLam, SKLen_HK
dc.contributor.authorChan, SCWen_HK
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorLuk, KDKen_HK
dc.date.accessioned2012-09-20T07:59:32Z-
dc.date.available2012-09-20T07:59:32Z-
dc.date.issued2011en_HK
dc.identifier.citationEuropean Cells And Materials, 2011, v. 22, p. 393-402en_HK
dc.identifier.issn1473-2262en_HK
dc.identifier.urihttp://hdl.handle.net/10722/164430-
dc.description.abstractImplantation of intervertebral disc (IVD) allograft or tissue engineered disc constructs in the spine has emerged as an alternative to artificial disc replacement for the treatment of severe degenerative disc disease (DDD). Establishment of a bank of cryopreserved IVD allografts enables size matching and facilitates logistics for effective clinical management. However, the biomechanical properties of cryopreserved IVDs have not been previously reported. This study aimed to assess if cryopreservation with different concentrations of cryopreservant agents (CPA) would affect the dynamic viscoelastic properties of the IVD. Whole porcine lumbar IVDs (n = 40) were harvested and processed using various concentrations of CPA, 0% CPA, 10% CPA and 20% CPA. The discs were cryopreserved using a stepwise freezing protocol and stored in liquid nitrogen. After four weeks of storage, the cryopreserved IVDs were quickly thawed at 37 °C for dynamic viscoelastic testing. The apparent modulus, elastic modulus (G"), viscous modulus (G") and loss modulus (G"/G') were calculated and compared to a fresh control group. Cryopreserved IVD without cryopreservants was significantly stiffer than the control. In the dynamic viscoelastic testing, cryopreservation with the use of CPA was able to preserve both G' and G" of an IVD. No significant differences were found between fresh IVD and IVD cryopreserved with 10% CPA or 20% CPA. This study demonstrated that CPAs at an optimal concentration could preserve the mechanical properties of the IVD allograft and can provide further credence for the application of long-term storage of IVD allografts for disc transplantation or tissue engineered construct applications.en_HK
dc.languageengen_US
dc.publisherSwiss Society for Biomaterials. The Journal's web site is located at http://www.ecmjournal.orgen_HK
dc.relation.ispartofEuropean Cells and Materialsen_HK
dc.subjectAllograften_HK
dc.subjectBiomechanicsen_HK
dc.subjectCryopreservationen_HK
dc.subjectIntervertebral discen_HK
dc.subjectTransplantationen_HK
dc.subject.meshBiomechanics-
dc.subject.meshCryopreservation-
dc.subject.meshElastic Modulus-
dc.subject.meshIntervertebral Disc-
dc.subject.meshSwine-
dc.titleThe role of cryopreservation in the biomechanical properties of the intervertebral discen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid22179937-
dc.identifier.scopuseid_2-s2.0-84857933873en_HK
dc.identifier.hkuros207339en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857933873&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.spage393en_HK
dc.identifier.epage402en_HK
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridLam, SKL=55631314600en_HK
dc.identifier.scopusauthoridChan, SCW=55209636300en_HK
dc.identifier.scopusauthoridLeung, VYL=35337438900en_HK
dc.identifier.scopusauthoridLu, WW=55763794518en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridLuk, KDK=55763794674en_HK
dc.customcontrol.immutablejt 130312-

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