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Article: Three-dimensional nanocomposite scaffolds for bone tissue engineering: from design to application

TitleThree-dimensional nanocomposite scaffolds for bone tissue engineering: from design to application
Authors
KeywordsBone tissue engineering
Scaffold
Selective laser sintering
Nanocomposite
Growth factor
Issue Date2012
PublisherWorld Scientific Publishing Co. Pte. Ltd. The Journal's web site is located at http://www.worldscinet.com/nl/nl.shtml
Citation
Nano LIFE, 2012, v. 2 n. 1, article no. 1250005 How to Cite?
AbstractSelective laser sintering (SLS), a rapid prototyping technology, was investigated for producing bone tissue engineering scaffolds. Completely biodegradable osteoconductive calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) scaffolds were successfully fabricated via SLS using Ca-P/PHBV nanocomposite microspheres. In the SLS manufacturing route, the architecture of tissue engineering scaffolds (pore shape, size, interconnectivity, etc.) can be designed and the sintering process can be optimized for obtaining scaffolds with desirable porous structures and mechanical properties. SLS was also shown to be very effective in producing highly complex porous structures using nanocomposite microspheres. To render SLS-formed Ca-P/PHBV scaffolds osteoinductive, recombinant human bone morphogenetic protein-2 (rhBMP-2) could be loaded onto the scaffolds. For achieving a controlled release of rhBMP-2 from scaffolds, surface modification of Ca-P/PHBV scaffolds by gelatin entrapment and heparin immobilization was needed. The immobilized heparin provided binding affinity for rhBMP-2. Surface modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 enhanced the proliferation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) and also their alkaline phosphatase activity. In in vivo experiments using a rabbit model, surface modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 promoted ectopic bone formation, exhibiting their osteoinductivity. The strategy of combining advanced scaffold fabrication, nanocomposite material, and controlled growth factor delivery is promising for bone tissue regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/164215
ISSN

 

DC FieldValueLanguage
dc.contributor.authorDuan, Ben_US
dc.contributor.authorWang, Men_US
dc.contributor.authorLu, WWen_US
dc.date.accessioned2012-09-20T07:56:39Z-
dc.date.available2012-09-20T07:56:39Z-
dc.date.issued2012en_US
dc.identifier.citationNano LIFE, 2012, v. 2 n. 1, article no. 1250005en_US
dc.identifier.issn1793-9844-
dc.identifier.urihttp://hdl.handle.net/10722/164215-
dc.description.abstractSelective laser sintering (SLS), a rapid prototyping technology, was investigated for producing bone tissue engineering scaffolds. Completely biodegradable osteoconductive calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) scaffolds were successfully fabricated via SLS using Ca-P/PHBV nanocomposite microspheres. In the SLS manufacturing route, the architecture of tissue engineering scaffolds (pore shape, size, interconnectivity, etc.) can be designed and the sintering process can be optimized for obtaining scaffolds with desirable porous structures and mechanical properties. SLS was also shown to be very effective in producing highly complex porous structures using nanocomposite microspheres. To render SLS-formed Ca-P/PHBV scaffolds osteoinductive, recombinant human bone morphogenetic protein-2 (rhBMP-2) could be loaded onto the scaffolds. For achieving a controlled release of rhBMP-2 from scaffolds, surface modification of Ca-P/PHBV scaffolds by gelatin entrapment and heparin immobilization was needed. The immobilized heparin provided binding affinity for rhBMP-2. Surface modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 enhanced the proliferation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) and also their alkaline phosphatase activity. In in vivo experiments using a rabbit model, surface modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 promoted ectopic bone formation, exhibiting their osteoinductivity. The strategy of combining advanced scaffold fabrication, nanocomposite material, and controlled growth factor delivery is promising for bone tissue regeneration.-
dc.languageengen_US
dc.publisherWorld Scientific Publishing Co. Pte. Ltd. The Journal's web site is located at http://www.worldscinet.com/nl/nl.shtmlen_US
dc.relation.ispartofNano LIFEen_US
dc.rightsNano LIFE. Copyright © World Scientific Publishing Co. Pte. Ltd.-
dc.rightsElectronic version of an article published as Nano LIFE, 2012, v. 2 n. 1, article no. 1250005. DOI: 10.1142/S1793984411000396-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBone tissue engineering-
dc.subjectScaffold-
dc.subjectSelective laser sintering-
dc.subjectNanocomposite-
dc.subjectGrowth factor-
dc.titleThree-dimensional nanocomposite scaffolds for bone tissue engineering: from design to applicationen_US
dc.typeArticleen_US
dc.identifier.emailWang, M: memwang@hku.hken_US
dc.identifier.emailLu, WW: wwlu@hku.hken_US
dc.identifier.authorityWang, M=rp00185en_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturepostprint-
dc.identifier.doi10.1142/S1793984411000396-
dc.identifier.hkuros207519en_US
dc.identifier.volume2en_US
dc.identifier.issue1, article no. 1250005-
dc.publisher.placeSingapore-

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