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Article: Effects of substrate loading and co-substrates on thermophilic anaerobic conversion of microcrystalline cellulose and microbial communities revealed using high-throughput sequencing

TitleEffects of substrate loading and co-substrates on thermophilic anaerobic conversion of microcrystalline cellulose and microbial communities revealed using high-throughput sequencing
Authors
KeywordsAnaerobic
Co-substrate
High-throughput
Illumina
Microcrystalline cellulose
Issue Date2012
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ijhydene
Citation
International Journal of Hydrogen Energy, 2012, v. 37 n. 18, p. 13652-13659 How to Cite?
AbstractBatch tests were conducted to investigate the effect of co-substrates, including glucose, xylose and starch, on thermophilic anaerobic conversion of microcrystalline cellulose using mixed culture enriched from anaerobic digestion sludge (ADS). Up to 30.9% of cellulose was utilized with xylose as co-substrate. When using glucose as co-substrate, cellulose conversion rate reached the maximum of 0.048 g/l/h at cellulose loading of 5.0 g/l. Illumina high-throughput sequencing of the 16S rRNA gene revealed that the thermophilic consortium exclusively consisted of Clostridium (more than 70% of all sequences). Growth of Thermoanaerobacterium over Clostridium would inhibit cellulose conversion capacity of the consortium. But the growth of Thermoanaerobacterium could be repressed by pH higher than pH 6.0. Co-substrates caused noticeable variation of bacterial community structure. Predominance of Thermoanaerobacterium over Clostridium was observed when monosugars (glucose and xylose) were used as co-substrate without pH control. Starch was ineffective as co-substrate because it competed with cellulose for Clostridium. Highlights: Co-substrate could affect cellulose conversion rate and community structure. Cellulose loading could affect cellulose conversion rate but not community structure. Clostridium was the cellulolytic constituent in the community. Thermoanaerobacterium growth over Clostridium inhibits cellulose conversion and repressed by pH > 6. Thermoanaerobacterium dominates when using monosugar co-substrate without pH control. © 2012 Hydrogen Energy Publications, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/163868
ISSN
2015 Impact Factor: 3.205
2015 SCImago Journal Rankings: 1.330
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXia, Yen_US
dc.contributor.authorCai, Len_US
dc.contributor.authorZhang, Ten_US
dc.contributor.authorFang, HHPen_US
dc.date.accessioned2012-09-20T07:52:37Z-
dc.date.available2012-09-20T07:52:37Z-
dc.date.issued2012en_US
dc.identifier.citationInternational Journal of Hydrogen Energy, 2012, v. 37 n. 18, p. 13652-13659en_US
dc.identifier.issn0360-3199-
dc.identifier.urihttp://hdl.handle.net/10722/163868-
dc.description.abstractBatch tests were conducted to investigate the effect of co-substrates, including glucose, xylose and starch, on thermophilic anaerobic conversion of microcrystalline cellulose using mixed culture enriched from anaerobic digestion sludge (ADS). Up to 30.9% of cellulose was utilized with xylose as co-substrate. When using glucose as co-substrate, cellulose conversion rate reached the maximum of 0.048 g/l/h at cellulose loading of 5.0 g/l. Illumina high-throughput sequencing of the 16S rRNA gene revealed that the thermophilic consortium exclusively consisted of Clostridium (more than 70% of all sequences). Growth of Thermoanaerobacterium over Clostridium would inhibit cellulose conversion capacity of the consortium. But the growth of Thermoanaerobacterium could be repressed by pH higher than pH 6.0. Co-substrates caused noticeable variation of bacterial community structure. Predominance of Thermoanaerobacterium over Clostridium was observed when monosugars (glucose and xylose) were used as co-substrate without pH control. Starch was ineffective as co-substrate because it competed with cellulose for Clostridium. Highlights: Co-substrate could affect cellulose conversion rate and community structure. Cellulose loading could affect cellulose conversion rate but not community structure. Clostridium was the cellulolytic constituent in the community. Thermoanaerobacterium growth over Clostridium inhibits cellulose conversion and repressed by pH > 6. Thermoanaerobacterium dominates when using monosugar co-substrate without pH control. © 2012 Hydrogen Energy Publications, LLC.-
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ijhydene-
dc.relation.ispartofInternational Journal of Hydrogen Energyen_US
dc.subjectAnaerobic-
dc.subjectCo-substrate-
dc.subjectHigh-throughput-
dc.subjectIllumina-
dc.subjectMicrocrystalline cellulose-
dc.titleEffects of substrate loading and co-substrates on thermophilic anaerobic conversion of microcrystalline cellulose and microbial communities revealed using high-throughput sequencingen_US
dc.typeArticleen_US
dc.identifier.emailCai, L: lcai@hku.hken_US
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_US
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.identifier.authorityFang, HHP=rp00115en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ijhydene.2012.02.079-
dc.identifier.scopuseid_2-s2.0-84865524626-
dc.identifier.hkuros208090en_US
dc.identifier.volume37-
dc.identifier.issue18-
dc.identifier.spage13652-
dc.identifier.epage13659-
dc.identifier.eissn1879-3487-
dc.identifier.isiWOS:000309043200051-
dc.publisher.placeUnited Kingdom-

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