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Article: Novel and conventional assays in determining abundance of Streptococcus mutans in saliva

TitleNovel and conventional assays in determining abundance of Streptococcus mutans in saliva
Authors
Issue Date2012
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439
Citation
International Journal of Paediatric Dentistry, 2012, v. 22 n. 5, p. 363-368 How to Cite?
AbstractBACKGROUND: Effective caries control and management requires identification of susceptible children for timely intervention. Streptococcus mutans (S. mutans) is an important biomarker of caries risk. AIM: This study aimed to comparatively evaluate the validities of a novel immunoassay and a conventional culture-based assay in detecting salivary S. mutans in a paediatric cohort. METHODS: 190 children aged 3-4 years were recruited. The abundance of S. mutans in their saliva samples was analysed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay system (Saliva-Check MUTANS) based on monoclonal antibody technology and a Taqman real-time PCR assay taken as a gold standard. RESULTS: The novel immunoassay accurately differentiated saliva samples with high (>/=5 x 10(5) CFU/mL) and low (<5 x 10(5) CFU/mL) S. mutans levels. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with moderate (>/=10(4) CFU/mL) S. mutans level. Its sensitivity/ specificity in selecting children with high (>/=10(5) CFU/mL) and very high (>10(6) CFU/mL) S. mutans levels were not sufficient (78.7%/79.8% and 25.8%/91.8%, respectively). CONCLUSION: The monoclonal antibody-based immunoassay accurately and rapidly determines S. mutans abundance in saliva and could be useful for chairside assessment of children's caries risk.
Persistent Identifierhttp://hdl.handle.net/10722/163747
ISSN
2015 Impact Factor: 1.303
2015 SCImago Journal Rankings: 0.721
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGao, XLen_US
dc.contributor.authorSeneviratne, CJen_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorChu, CHen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-09-20T07:50:38Z-
dc.date.available2012-09-20T07:50:38Z-
dc.date.issued2012en_US
dc.identifier.citationInternational Journal of Paediatric Dentistry, 2012, v. 22 n. 5, p. 363-368en_US
dc.identifier.issn0960-7439-
dc.identifier.urihttp://hdl.handle.net/10722/163747-
dc.description.abstractBACKGROUND: Effective caries control and management requires identification of susceptible children for timely intervention. Streptococcus mutans (S. mutans) is an important biomarker of caries risk. AIM: This study aimed to comparatively evaluate the validities of a novel immunoassay and a conventional culture-based assay in detecting salivary S. mutans in a paediatric cohort. METHODS: 190 children aged 3-4 years were recruited. The abundance of S. mutans in their saliva samples was analysed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay system (Saliva-Check MUTANS) based on monoclonal antibody technology and a Taqman real-time PCR assay taken as a gold standard. RESULTS: The novel immunoassay accurately differentiated saliva samples with high (>/=5 x 10(5) CFU/mL) and low (<5 x 10(5) CFU/mL) S. mutans levels. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with moderate (>/=10(4) CFU/mL) S. mutans level. Its sensitivity/ specificity in selecting children with high (>/=10(5) CFU/mL) and very high (>10(6) CFU/mL) S. mutans levels were not sufficient (78.7%/79.8% and 25.8%/91.8%, respectively). CONCLUSION: The monoclonal antibody-based immunoassay accurately and rapidly determines S. mutans abundance in saliva and could be useful for chairside assessment of children's caries risk.-
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439-
dc.relation.ispartofInternational Journal of Paediatric Dentistryen_US
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.titleNovel and conventional assays in determining abundance of Streptococcus mutans in salivaen_US
dc.typeArticleen_US
dc.identifier.emailGao, XL: gaoxl@hkucc.hku.hken_US
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.authorityGao, X=rp01509en_US
dc.identifier.authoritySeneviratne, CJ=rp01372en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-263X.2011.01207.x-
dc.identifier.pmid22211704-
dc.identifier.scopuseid_2-s2.0-84864946276-
dc.identifier.hkuros210380en_US
dc.identifier.volume22en_US
dc.identifier.issue5en_US
dc.identifier.spage363en_US
dc.identifier.epage368en_US
dc.identifier.eissn1365-263X-
dc.identifier.isiWOS:000307308900008-
dc.publisher.placeUnited Kingdom-

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