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Article: Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.

TitleExpression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.
Authors
Issue Date2004
PublisherPeking University Health Science Center. The Journal's web site is located at http://xuebao.bjmu.edu.cn/
Citation
Beijing Da Xue Xue Bao. Yi Xue Ban = Journal Of Peking University. Health Sciences, 2004, v. 36 n. 6, p. 646-649 How to Cite?
AbstractOBJECTIVE: To study the expression of osteoprotegerin(OPG) and receptor activator nuclear factor kappa B ligand(RANKL) at protein level in human periodontal ligament cells (HPDLCs), and the effect of 1alpha,25(OH)(2) vitamin D(3) [1,25(OH)(2)vitD(3)] on the secretion of OPG protein in vitro. METHODS: HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase. The expression of RANKL in HPDLCs at protein level was tested by immunocyto-chemistry. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the OPG protein which was secreted into the culture medium by HPDLCs cultured with and without 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3) on the 0, 2nd, 4th, and 6th days, respectively. RESULTS: RANKL protein was detected on the membrane and plasma of HPDLCs, and OPG protein was secreted in the culture medium. The secretion of OPG protein was down-regulated by 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3). CONCLUSION: HPDLCs have the bone metabolism system of OPG/RANKL, which works during the process of 1alpha,25 (OH)(2)vitD(3) inducing HPDLCs. The conclusion has laid the groundwork for the study on bone remodelling mechanisms of HPDLCs.
Persistent Identifierhttp://hdl.handle.net/10722/154516
ISSN
2015 SCImago Journal Rankings: 0.144

 

DC FieldValueLanguage
dc.contributor.authorZhang, Den_US
dc.contributor.authorYang, YQen_US
dc.contributor.authorLi, XTen_US
dc.contributor.authorFu, MKen_US
dc.date.accessioned2012-08-08T08:25:54Z-
dc.date.available2012-08-08T08:25:54Z-
dc.date.issued2004en_US
dc.identifier.citationBeijing Da Xue Xue Bao. Yi Xue Ban = Journal Of Peking University. Health Sciences, 2004, v. 36 n. 6, p. 646-649en_US
dc.identifier.issn1671-167Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/154516-
dc.description.abstractOBJECTIVE: To study the expression of osteoprotegerin(OPG) and receptor activator nuclear factor kappa B ligand(RANKL) at protein level in human periodontal ligament cells (HPDLCs), and the effect of 1alpha,25(OH)(2) vitamin D(3) [1,25(OH)(2)vitD(3)] on the secretion of OPG protein in vitro. METHODS: HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase. The expression of RANKL in HPDLCs at protein level was tested by immunocyto-chemistry. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the OPG protein which was secreted into the culture medium by HPDLCs cultured with and without 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3) on the 0, 2nd, 4th, and 6th days, respectively. RESULTS: RANKL protein was detected on the membrane and plasma of HPDLCs, and OPG protein was secreted in the culture medium. The secretion of OPG protein was down-regulated by 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3). CONCLUSION: HPDLCs have the bone metabolism system of OPG/RANKL, which works during the process of 1alpha,25 (OH)(2)vitD(3) inducing HPDLCs. The conclusion has laid the groundwork for the study on bone remodelling mechanisms of HPDLCs.en_US
dc.languageengen_US
dc.publisherPeking University Health Science Center. The Journal's web site is located at http://xuebao.bjmu.edu.cn/en_US
dc.relation.ispartofBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciencesen_US
dc.subject.meshCalcifediol - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshOsteoprotegerin - Biosynthesisen_US
dc.subject.meshPeriodontal Ligament - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshRank Ligand - Biosynthesisen_US
dc.titleExpression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.en_US
dc.typeArticleen_US
dc.identifier.emailYang, YQ:yangyanq@hkucc.hku.hken_US
dc.identifier.authorityYang, YQ=rp00045en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid15605102-
dc.identifier.scopuseid_2-s2.0-44249092609en_US
dc.identifier.hkuros146514-
dc.identifier.volume36en_US
dc.identifier.issue6en_US
dc.identifier.spage646en_US
dc.identifier.epage649en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridZhang, D=7405356257en_US
dc.identifier.scopusauthoridYang, YQ=36623085300en_US
dc.identifier.scopusauthoridLi, XT=7501710811en_US
dc.identifier.scopusauthoridFu, MK=7202031177en_US

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