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Article: Alteration of immunological properties of bacterial lipopolysaccharide by calcium hydroxide treatment

TitleAlteration of immunological properties of bacterial lipopolysaccharide by calcium hydroxide treatment
Authors
Issue Date2001
PublisherChinese Medical Association (中華醫學會). The Journal's web site is located at http://zhkqyx.periodicals.net.cn/default.html
Citation
Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal Of Stomatology, 2001, v. 36 n. 6, p. 417-420 How to Cite?
AbstractOBJECTIVE: To study alteration of immunological properties of LPS by Ca(OH)2 treatment, and to establish an experimental model in which ThP-1 cell line was cultured in vitro to observe the alteration of immunological properties of LPS. METHODS: ThP-1 cell line was used to establish a culture model in vitro. Amount of TNF-alpha released from stimulated cells acted as the index on immunological properties of the stimuli and alteration of immunological properties of LPS by Ca(OH)2 treatment. RESULTS: Within the range of observed LPS concentrations (10 micrograms/L-10 mg/L), the original immunological properties of LPS decreased significantly after treatment by Ca(OH)2 for a week (P < 0.001). When pH > or = 12.30, the amount of TNF-alpha excreted by monocyte did not differ significantly from the control group (P > 0.05). CONCLUSIONS: After treatment by Ca(OH)2 for a week, the original immunological properties of LPS decreased significantly. High pH value of Ca(OH)2 played a critical role during the alteration of immunological properties of LPS.
Persistent Identifierhttp://hdl.handle.net/10722/154169
ISSN
2015 SCImago Journal Rankings: 0.121

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorLin, Qen_US
dc.date.accessioned2012-08-08T08:23:39Z-
dc.date.available2012-08-08T08:23:39Z-
dc.date.issued2001en_US
dc.identifier.citationZhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal Of Stomatology, 2001, v. 36 n. 6, p. 417-420en_US
dc.identifier.issn1002-0098en_US
dc.identifier.urihttp://hdl.handle.net/10722/154169-
dc.description.abstractOBJECTIVE: To study alteration of immunological properties of LPS by Ca(OH)2 treatment, and to establish an experimental model in which ThP-1 cell line was cultured in vitro to observe the alteration of immunological properties of LPS. METHODS: ThP-1 cell line was used to establish a culture model in vitro. Amount of TNF-alpha released from stimulated cells acted as the index on immunological properties of the stimuli and alteration of immunological properties of LPS by Ca(OH)2 treatment. RESULTS: Within the range of observed LPS concentrations (10 micrograms/L-10 mg/L), the original immunological properties of LPS decreased significantly after treatment by Ca(OH)2 for a week (P < 0.001). When pH > or = 12.30, the amount of TNF-alpha excreted by monocyte did not differ significantly from the control group (P > 0.05). CONCLUSIONS: After treatment by Ca(OH)2 for a week, the original immunological properties of LPS decreased significantly. High pH value of Ca(OH)2 played a critical role during the alteration of immunological properties of LPS.en_US
dc.languageengen_US
dc.publisherChinese Medical Association (中華醫學會). The Journal's web site is located at http://zhkqyx.periodicals.net.cn/default.htmlen_US
dc.relation.ispartofZhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatologyen_US
dc.subject.meshCalcium Hydroxide - Pharmacologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshLipopolysaccharides - Pharmacologyen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Biosynthesisen_US
dc.titleAlteration of immunological properties of bacterial lipopolysaccharide by calcium hydroxide treatmenten_US
dc.typeArticleen_US
dc.identifier.emailZhang, C:zhangcf@hku.hken_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11930713-
dc.identifier.scopuseid_2-s2.0-0035524419en_US
dc.identifier.volume36en_US
dc.identifier.issue6en_US
dc.identifier.spage417en_US
dc.identifier.epage420en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWang, X=8407096000en_US
dc.identifier.scopusauthoridZhang, C=7405494609en_US
dc.identifier.scopusauthoridLin, Q=36864656400en_US

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