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Article: H3K27me3 protein is a promising predictive biomarker of patients' survival and chemoradioresistance in human nasopharyngeal carcinoma
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TitleH3K27me3 protein is a promising predictive biomarker of patients' survival and chemoradioresistance in human nasopharyngeal carcinoma
 
AuthorsCai, MY2
Tong, ZT2 1
Zhu, W2
Wen, ZZ2
Rao, HL2
Kong, LL1
Guan, XY2
Kung, HF2 3
Zeng, YX2
Xie, D2
 
Issue Date2011
 
PublisherThe Feinstein Institute for Medical Research. The Journal's web site is located at http://www.molmed.org
 
CitationMolecular Medicine, 2011, v. 17 n. 11, p. 1137-1145 [How to Cite?]
DOI: http://dx.doi.org/10.2119/molmed.2011.00054
 
AbstractTrimethylation of lysine 27 on histone H3 (H3K27me3) is an epigenetic change which plays a critical role in tumor development and/or progression. However, the molecular status of H3K27me3 and its clinicopathologic/prognostic significance in nasopharyngeal carcinoma (NPC) have not been elucidated. In this study, the methods of Western blotting and immunohistochemistry (IHC) were utilized to examine the expression of H3K27me3 protein in NPC tissues and nonneoplastic nasopharyngeal epithelial tissues. Receiver operating characteristic (ROC) curve analysis was used to determine the cutpoint for H3K27me3 high expression. High expression of H3K27me3 could be observed in 127/209 (60.8%) of NPCs and in 8/50 (16.0%) normal nasopharyngeal epithelial tissues (P < 0.001). Further correlation analysis demonstrated that high expression of H3K27me3 was positively associated with tumor later T classification, tumor metastasis, advanced clinical stage and chemoradioresistance (P < 0.05). Moreover, high expression of H3K27me3 was closely associated with NPC patient shortened survival time as evidenced by univariate and multivariate analysis (P < 0.05). Consequently, a new clinicopathologic prognostic model with three poor prognostic factors (H3K27me3 expression, distant metastasis and treatment regimen) was constructed. The model could stratify risk significantly (low, intermediate and high) for overall survival and progression-free survival (P < 0.0001). These findings provide evidence that H3K27me3 expression, as examined by IHC, has the potential to be used as an immunomarker to predict NPC chemoradiotherapy response and patient prognosis. The combined clinicopathologic prognostic model may become a useful tool for identifying NPC patients with different clinical outcomes. © 2011 The Feinstein Institute for Medical Research.
 
ISSN1076-1551
2013 Impact Factor: 4.824
 
DOIhttp://dx.doi.org/10.2119/molmed.2011.00054
 
ISI Accession Number IDWOS:000297958800004
Funding AgencyGrant Number
973 Project of China2010CB912802
2010CB529401
Foundation of Guangzhou Science and Technology Bureau, China2005Z1-E0131
Funding Information:

This work was supported by the 973 Project of China (2010CB912802 and 2010CB529401) and the Foundation of Guangzhou Science and Technology Bureau, China (2005Z1-E0131).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCai, MY
 
dc.contributor.authorTong, ZT
 
dc.contributor.authorZhu, W
 
dc.contributor.authorWen, ZZ
 
dc.contributor.authorRao, HL
 
dc.contributor.authorKong, LL
 
dc.contributor.authorGuan, XY
 
dc.contributor.authorKung, HF
 
dc.contributor.authorZeng, YX
 
dc.contributor.authorXie, D
 
dc.date.accessioned2012-06-26T06:12:08Z
 
dc.date.available2012-06-26T06:12:08Z
 
dc.date.issued2011
 
dc.description.abstractTrimethylation of lysine 27 on histone H3 (H3K27me3) is an epigenetic change which plays a critical role in tumor development and/or progression. However, the molecular status of H3K27me3 and its clinicopathologic/prognostic significance in nasopharyngeal carcinoma (NPC) have not been elucidated. In this study, the methods of Western blotting and immunohistochemistry (IHC) were utilized to examine the expression of H3K27me3 protein in NPC tissues and nonneoplastic nasopharyngeal epithelial tissues. Receiver operating characteristic (ROC) curve analysis was used to determine the cutpoint for H3K27me3 high expression. High expression of H3K27me3 could be observed in 127/209 (60.8%) of NPCs and in 8/50 (16.0%) normal nasopharyngeal epithelial tissues (P < 0.001). Further correlation analysis demonstrated that high expression of H3K27me3 was positively associated with tumor later T classification, tumor metastasis, advanced clinical stage and chemoradioresistance (P < 0.05). Moreover, high expression of H3K27me3 was closely associated with NPC patient shortened survival time as evidenced by univariate and multivariate analysis (P < 0.05). Consequently, a new clinicopathologic prognostic model with three poor prognostic factors (H3K27me3 expression, distant metastasis and treatment regimen) was constructed. The model could stratify risk significantly (low, intermediate and high) for overall survival and progression-free survival (P < 0.0001). These findings provide evidence that H3K27me3 expression, as examined by IHC, has the potential to be used as an immunomarker to predict NPC chemoradiotherapy response and patient prognosis. The combined clinicopathologic prognostic model may become a useful tool for identifying NPC patients with different clinical outcomes. © 2011 The Feinstein Institute for Medical Research.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationMolecular Medicine, 2011, v. 17 n. 11, p. 1137-1145 [How to Cite?]
DOI: http://dx.doi.org/10.2119/molmed.2011.00054
 
dc.identifier.doihttp://dx.doi.org/10.2119/molmed.2011.00054
 
dc.identifier.epage1145
 
dc.identifier.hkuros203483
 
dc.identifier.isiWOS:000297958800004
Funding AgencyGrant Number
973 Project of China2010CB912802
2010CB529401
Foundation of Guangzhou Science and Technology Bureau, China2005Z1-E0131
Funding Information:

This work was supported by the 973 Project of China (2010CB912802 and 2010CB529401) and the Foundation of Guangzhou Science and Technology Bureau, China (2005Z1-E0131).

 
dc.identifier.issn1076-1551
2013 Impact Factor: 4.824
 
dc.identifier.issue11
 
dc.identifier.pmid21738951
 
dc.identifier.scopuseid_2-s2.0-82855172243
 
dc.identifier.spage1137
 
dc.identifier.urihttp://hdl.handle.net/10722/150844
 
dc.identifier.volume17
 
dc.languageeng
 
dc.publisherThe Feinstein Institute for Medical Research. The Journal's web site is located at http://www.molmed.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofMolecular Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.titleH3K27me3 protein is a promising predictive biomarker of patients' survival and chemoradioresistance in human nasopharyngeal carcinoma
 
dc.typeArticle
 
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<contributor.author>Rao, HL</contributor.author>
<contributor.author>Kong, LL</contributor.author>
<contributor.author>Guan, XY</contributor.author>
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<contributor.author>Zeng, YX</contributor.author>
<contributor.author>Xie, D</contributor.author>
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Author Affiliations
  1. Anhui Medical University
  2. Sun Yat-Sen University
  3. Chinese University of Hong Kong