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Article: Organochlorine isotopic pattern-enhanced detection and quantification of triclosan and its metabolites in human serum by ultra-high-performance liquid chromatography/quadrupole time-of-flight/mass spectrometry

TitleOrganochlorine isotopic pattern-enhanced detection and quantification of triclosan and its metabolites in human serum by ultra-high-performance liquid chromatography/quadrupole time-of-flight/mass spectrometry
Authors
Issue Date2012
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0951-4198/
Citation
Rapid Communications In Mass Spectrometry, 2012, v. 26 n. 2, p. 123-132 How to Cite?
AbstractOrganochlorines possess special isotopic patterns that obey the chlorine rule. In the case of triclosan (TCS), which contains three chlorine atoms, the isotopic patterns are composed of seven obvious peaks with the calculated masses ranging from 286.9435 to 292.9350 in negative ion mode and with specific isotopic abundance ratios of 100:13.1:97.1:12.6:31.8:4.1:3.6. In this study, mass differences between the calculated and observed m/z values for all isotopic peaks of TCS were less than 3.5 ppm in the analyses of the serum samples by ultra-high-performance liquid chromatography/quadrupole time-of-flight/mass spectrometry (UHPLC-Q-TOF/MS). Combining the characteristics described above, four metabolites were identified as sulfonated TCS, glucuronidated TCS and hydroxylated sulfonated TCS. Several novel MS techniques were applied to improve the sensitivity of quantification of TCS. The limit of detection for TCS in a 250 μL serum sample was 0.05 ng/mL, which was over twenty times lower than values obtained by the LC/triple quadrupole-MS/MS method reported in the literature. The concentration of total TCS (free and conjugated) was quantified to range from 0.15 to 217 ng/mL, whereas free TCS ranged from 0.15 to 10 ng/mL. To the best of our knowledge, this is the first report on the identification of TCS and metabolites in human serum, and it also provides the most sensitive LC/MS approach for the quantification of TCS. Copyright © 2011 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/148672
ISSN
2015 Impact Factor: 2.226
2015 SCImago Journal Rankings: 0.819
ISI Accession Number ID
Funding AgencyGrant Number
Research & Conference Grants Administration System
University of Hong Kong201007176089
201007176082
Funding Information:

We thank the Research & Conference Grants Administration System and the Small Project Funding (201007176089 and 201007176082) of The University of Hong Kong for their financial support.

References

 

DC FieldValueLanguage
dc.contributor.authorWu, JLen_US
dc.contributor.authorLeung, KFen_US
dc.contributor.authorTong, SFen_US
dc.contributor.authorLam, CWen_US
dc.date.accessioned2012-05-29T06:14:34Z-
dc.date.available2012-05-29T06:14:34Z-
dc.date.issued2012en_US
dc.identifier.citationRapid Communications In Mass Spectrometry, 2012, v. 26 n. 2, p. 123-132en_US
dc.identifier.issn0951-4198en_US
dc.identifier.urihttp://hdl.handle.net/10722/148672-
dc.description.abstractOrganochlorines possess special isotopic patterns that obey the chlorine rule. In the case of triclosan (TCS), which contains three chlorine atoms, the isotopic patterns are composed of seven obvious peaks with the calculated masses ranging from 286.9435 to 292.9350 in negative ion mode and with specific isotopic abundance ratios of 100:13.1:97.1:12.6:31.8:4.1:3.6. In this study, mass differences between the calculated and observed m/z values for all isotopic peaks of TCS were less than 3.5 ppm in the analyses of the serum samples by ultra-high-performance liquid chromatography/quadrupole time-of-flight/mass spectrometry (UHPLC-Q-TOF/MS). Combining the characteristics described above, four metabolites were identified as sulfonated TCS, glucuronidated TCS and hydroxylated sulfonated TCS. Several novel MS techniques were applied to improve the sensitivity of quantification of TCS. The limit of detection for TCS in a 250 μL serum sample was 0.05 ng/mL, which was over twenty times lower than values obtained by the LC/triple quadrupole-MS/MS method reported in the literature. The concentration of total TCS (free and conjugated) was quantified to range from 0.15 to 217 ng/mL, whereas free TCS ranged from 0.15 to 10 ng/mL. To the best of our knowledge, this is the first report on the identification of TCS and metabolites in human serum, and it also provides the most sensitive LC/MS approach for the quantification of TCS. Copyright © 2011 John Wiley & Sons, Ltd.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0951-4198/en_US
dc.relation.ispartofRapid Communications in Mass Spectrometryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshDolphinsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrocarbons, Chlorinated - Blood - Chemistryen_US
dc.subject.meshIsotopesen_US
dc.subject.meshLimit Of Detectionen_US
dc.subject.meshMass Spectrometry - Methodsen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshTriclosan - Blood - Chemistryen_US
dc.titleOrganochlorine isotopic pattern-enhanced detection and quantification of triclosan and its metabolites in human serum by ultra-high-performance liquid chromatography/quadrupole time-of-flight/mass spectrometryen_US
dc.typeArticleen_US
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_US
dc.identifier.authorityLam, CW=rp00260en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/rcm.5303en_US
dc.identifier.pmid22173800-
dc.identifier.scopuseid_2-s2.0-83755163205en_US
dc.identifier.hkuros201623-
dc.identifier.hkuros208502-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-83755163205&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume26en_US
dc.identifier.issue2en_US
dc.identifier.spage123en_US
dc.identifier.epage132en_US
dc.identifier.isiWOS:000299738500004-
dc.publisher.placeUnited Kingdomen_US

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