Article: Epigenetic disruption of interferon-γ response through silencing the tumor suppressor interferon regulatory factor 8 in nasopharyngeal, esophageal and multiple other carcinomas
| Title | Epigenetic disruption of interferon-γ response through silencing the tumor suppressor interferon regulatory factor 8 in nasopharyngeal, esophageal and multiple other carcinomas | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Lee, KY4 Geng, H4 Ng, KM4 Yu, J8 Van Hasselt, A8 Cao, Y6 Zeng, YX1 Wong, AHY4 Wang, X4 Ying, J4 Srivastava, G2 Lung, ML5 Wang, LD3 Kwok, TT8 Levi, BZ7 Chan, ATC4 Sung, JJY8 Tao, Q4 | ||||||
| Keywords | Carcinoma CpG island IRF8 Methylation Tumor suppressor gene | ||||||
| Issue Date | 2008 | ||||||
| Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/onc | ||||||
| Citation | Oncogene, 2008, v. 27 n. 39, p. 5267-5276 [How to Cite?] DOI: http://dx.doi.org/10.1038/onc.2008.147 | ||||||
| Abstract | 16q24 is frequently deleted in multiple tumors including cancers of nasopharynx, esophagus, breast, prostate and liver. By array comparative genomic hybridization (aCGH), we refined a 16q24 hemizygous deletion in nasopharyngeal carcinoma (NPC) cell lines. Semi-quantitative RT-PCR analysis revealed interferon regulatory factor 8 (IRF8) as the only downregulated gene within this deletion. IRF8 belongs to a family of interferon (IFN) regulatory factors that modulate various important physiologic processes including host defense, cell growth and differentiation and immune regulation. In contrast to the broad expression of IRF8 in normal adult and fetal tissues, transcriptional silencing and promoter methylation of IRF8 were frequently detected in multiple carcinoma (except for hepatocellular) cell lines (100% in NPC, 88% in esophageal and 18-78% in other carcinoma cell lines) and in a large collection of primary carcinomas (78% in NPC, 36-71% in other carcinomas). Methylation of the IRF8 promoter led to the disruption of its response to IFN-γ stimulation. Pharmacological and genetic demethylation could restore IRF8 expression, indicating a direct epigenetic mechanism. Ectopic expression of IRF8 in tumor cells lacking its expression strongly inhibited their clonogenicity, confirming its tumor suppressor function. Thus, IRF8 was identified as a functional tumor suppressor, which is frequently silenced by epigenetic mechanism in multiple carcinomas. © 2008 Macmillan Publishers Limited All rights reserved. | ||||||
| ISSN | 0950-9232 2011 Impact Factor: 6.373 2011 SCImago Journal Rankings: 1.216 | ||||||
| DOI | http://dx.doi.org/10.1038/onc.2008.147 | ||||||
| ISI Accession Number ID | WOS:000258915100011
Funding Information: This project was supported by a Michael and Betty Kadoorie Cancer Genetics Research Program (MBKCGRP) Grant to QT and a Hong Kong RGC Central Allocation Grant (CA06/07.SC03, QT). We thank Drs Bert Vogelstein, George Tsao (Dolly Huang), Sun Young Rha and Kaitai Yao for some cell lines, DSMZ (German Collection of Microorganisms and Cell Cultures) for the KYSE cell lines [Shimada et al., Cancer 69: 277-284 (1992)], Dr C Langford at the Wellcome Trust Sanger Institute, Cambridge, UK for aCGH slides, and Tzer- Jing Seng (Johns Hopkins Singapore) for her valuable help in aCGH analysis. | ||||||
| References | References in Scopus |
| dc.contributor.author | Lee, KY | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Geng, H | ||||||
| dc.contributor.author | Ng, KM | ||||||
| dc.contributor.author | Yu, J | ||||||
| dc.contributor.author | Van Hasselt, A | ||||||
| dc.contributor.author | Cao, Y | ||||||
| dc.contributor.author | Zeng, YX | ||||||
| dc.contributor.author | Wong, AHY | ||||||
| dc.contributor.author | Wang, X | ||||||
| dc.contributor.author | Ying, J | ||||||
| dc.contributor.author | Srivastava, G | ||||||
| dc.contributor.author | Lung, ML | ||||||
| dc.contributor.author | Wang, LD | ||||||
| dc.contributor.author | Kwok, TT | ||||||
| dc.contributor.author | Levi, BZ | ||||||
| dc.contributor.author | Chan, ATC | ||||||
| dc.contributor.author | Sung, JJY | ||||||
| dc.contributor.author | Tao, Q | ||||||
| dc.date.accessioned | 2012-05-29T06:13:51Z | ||||||
| dc.date.available | 2012-05-29T06:13:51Z | ||||||
| dc.date.issued | 2008 | ||||||
| dc.description.abstract | 16q24 is frequently deleted in multiple tumors including cancers of nasopharynx, esophagus, breast, prostate and liver. By array comparative genomic hybridization (aCGH), we refined a 16q24 hemizygous deletion in nasopharyngeal carcinoma (NPC) cell lines. Semi-quantitative RT-PCR analysis revealed interferon regulatory factor 8 (IRF8) as the only downregulated gene within this deletion. IRF8 belongs to a family of interferon (IFN) regulatory factors that modulate various important physiologic processes including host defense, cell growth and differentiation and immune regulation. In contrast to the broad expression of IRF8 in normal adult and fetal tissues, transcriptional silencing and promoter methylation of IRF8 were frequently detected in multiple carcinoma (except for hepatocellular) cell lines (100% in NPC, 88% in esophageal and 18-78% in other carcinoma cell lines) and in a large collection of primary carcinomas (78% in NPC, 36-71% in other carcinomas). Methylation of the IRF8 promoter led to the disruption of its response to IFN-γ stimulation. Pharmacological and genetic demethylation could restore IRF8 expression, indicating a direct epigenetic mechanism. Ectopic expression of IRF8 in tumor cells lacking its expression strongly inhibited their clonogenicity, confirming its tumor suppressor function. Thus, IRF8 was identified as a functional tumor suppressor, which is frequently silenced by epigenetic mechanism in multiple carcinomas. © 2008 Macmillan Publishers Limited All rights reserved. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Oncogene, 2008, v. 27 n. 39, p. 5267-5276 [How to Cite?] DOI: http://dx.doi.org/10.1038/onc.2008.147 | ||||||
| dc.identifier.citeulike | 2791205 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1038/onc.2008.147 | ||||||
| dc.identifier.epage | 5276 | ||||||
| dc.identifier.hkuros | 150716 | ||||||
| dc.identifier.isi | WOS:000258915100011
Funding Information: This project was supported by a Michael and Betty Kadoorie Cancer Genetics Research Program (MBKCGRP) Grant to QT and a Hong Kong RGC Central Allocation Grant (CA06/07.SC03, QT). We thank Drs Bert Vogelstein, George Tsao (Dolly Huang), Sun Young Rha and Kaitai Yao for some cell lines, DSMZ (German Collection of Microorganisms and Cell Cultures) for the KYSE cell lines [Shimada et al., Cancer 69: 277-284 (1992)], Dr C Langford at the Wellcome Trust Sanger Institute, Cambridge, UK for aCGH slides, and Tzer- Jing Seng (Johns Hopkins Singapore) for her valuable help in aCGH analysis. | ||||||
| dc.identifier.issn | 0950-9232 2011 Impact Factor: 6.373 2011 SCImago Journal Rankings: 1.216 | ||||||
| dc.identifier.issue | 39 | ||||||
| dc.identifier.pmid | 18469857 | ||||||
| dc.identifier.scopus | eid_2-s2.0-51349083104 | ||||||
| dc.identifier.spage | 5267 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/148578 | ||||||
| dc.identifier.volume | 27 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/onc | ||||||
| dc.publisher.place | United Kingdom | ||||||
| dc.relation.ispartof | Oncogene | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Cell Line, Tumor | ||||||
| dc.subject.mesh | Dna Methylation | ||||||
| dc.subject.mesh | Down-Regulation | ||||||
| dc.subject.mesh | Epigenesis, Genetic | ||||||
| dc.subject.mesh | Esophageal Neoplasms - Genetics | ||||||
| dc.subject.mesh | Gene Silencing | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Interferon Regulatory Factors - Genetics | ||||||
| dc.subject.mesh | Interferon-Gamma - Physiology | ||||||
| dc.subject.mesh | Nasopharyngeal Neoplasms - Genetics | ||||||
| dc.subject.mesh | Promoter Regions, Genetic | ||||||
| dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | ||||||
| dc.subject | Carcinoma | ||||||
| dc.subject | CpG island | ||||||
| dc.subject | IRF8 | ||||||
| dc.subject | Methylation | ||||||
| dc.subject | Tumor suppressor gene | ||||||
| dc.title | Epigenetic disruption of interferon-γ response through silencing the tumor suppressor interferon regulatory factor 8 in nasopharyngeal, esophageal and multiple other carcinomas | ||||||
| dc.type | Article |
Author Affiliations
- Sun Yat-Sen University Cancer Center
- The University of Hong Kong
- Zhengzhou University
- Prince of Wales Hospital Hong Kong
- Hong Kong University of Science and Technology
- Central South University China
- Technion - Israel Institute of Technology
- Chinese University of Hong Kong