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Article: Anti-heat-shock protein 70 kDa antibodies in vascular patients

TitleAnti-heat-shock protein 70 kDa antibodies in vascular patients
Authors
KeywordsAneurysm
Antibodies
Critical ischaemia
ELISA
Heat-shock protein 70
Intermittent claudication
Issue Date1999
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ejvs
Citation
European Journal Of Vascular And Endovascular Surgery, 1999, v. 18 n. 5, p. 381-385 How to Cite?
AbstractIntroduction and aim of study: there is recent evidence that the immune system plays an essential role in the pathogenesis of atherosclerosis, with both cellular and humoral mechanisms being involved. Heat-shock proteins (HSPs) have been detected in atherosclerotic lesions, and antibodies to HSPs have also been found to be raised in patients with carotid stenoses. The aim of our study was to examine the level of anti-HSP70 antibodies in patients with other vascular diseases. Materials and methods: a questionnaire was designed for the subjects in the study, with documentation of clinical details and ankle-brachial pressure index. Patients with concomitant infection, malignancy, hepatorenal failure, or recent surgery were excluded. Enzyme-linked immunosorbent assay (ELISA) was used to identify anti-HSP70 antibodies in the sera in different dilutions. Graphs of optical density (OD) vs. negative log dilution were plotted, the gradient of which was taken to be the estimated optical density for each subject (proportional to antibody level). Our groups consisted of controls (n = 21, mean age 59.0 ± 19.2), lower limb claudicants (n = 19, mean age 60.0 ± 12.6), patients with lower-limb critical ischaemia (n = 22, mean age 68.5 ± 10.07), and patients with abdominal aortic aneurysms ((n = 20, mean age 69.9 ± 6.2). Results: we found no correlation between age and the estimated OD in our subjects (Spearman's correlation coefficient (r) = 0.123, one-tailed p value was 0.135). Patients with intermittent claudication, critical lower limb ischaemia, and aneurysms had higher estimated OD, and therefore higher anti-HSP70 antibody levels, than controls (Mann-Whitney test; p = 0.0127, 0.0037, 0.0008, respectively). Conclusions: our data provide the first evidence of a correlation between anti-HSP70 antibodies and different types of vascular diseases, suggesting that HSP70 might be involved in the pathogenesis and propagation of atherosclerosis. Since the immune response to HSPs can be modulated, this opens up the possibility of new therapeutic approaches.
Persistent Identifierhttp://hdl.handle.net/10722/147673
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.723
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, YCen_HK
dc.contributor.authorShukla, Nen_HK
dc.contributor.authorAbdusSamee, Men_HK
dc.contributor.authorBerwanger, CSen_HK
dc.contributor.authorStanford, Jen_HK
dc.contributor.authorSingh, Men_HK
dc.contributor.authorMansfield, AOen_HK
dc.contributor.authorStanby, Gen_HK
dc.date.accessioned2012-05-29T06:07:51Z-
dc.date.available2012-05-29T06:07:51Z-
dc.date.issued1999en_HK
dc.identifier.citationEuropean Journal Of Vascular And Endovascular Surgery, 1999, v. 18 n. 5, p. 381-385en_HK
dc.identifier.issn1078-5884en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147673-
dc.description.abstractIntroduction and aim of study: there is recent evidence that the immune system plays an essential role in the pathogenesis of atherosclerosis, with both cellular and humoral mechanisms being involved. Heat-shock proteins (HSPs) have been detected in atherosclerotic lesions, and antibodies to HSPs have also been found to be raised in patients with carotid stenoses. The aim of our study was to examine the level of anti-HSP70 antibodies in patients with other vascular diseases. Materials and methods: a questionnaire was designed for the subjects in the study, with documentation of clinical details and ankle-brachial pressure index. Patients with concomitant infection, malignancy, hepatorenal failure, or recent surgery were excluded. Enzyme-linked immunosorbent assay (ELISA) was used to identify anti-HSP70 antibodies in the sera in different dilutions. Graphs of optical density (OD) vs. negative log dilution were plotted, the gradient of which was taken to be the estimated optical density for each subject (proportional to antibody level). Our groups consisted of controls (n = 21, mean age 59.0 ± 19.2), lower limb claudicants (n = 19, mean age 60.0 ± 12.6), patients with lower-limb critical ischaemia (n = 22, mean age 68.5 ± 10.07), and patients with abdominal aortic aneurysms ((n = 20, mean age 69.9 ± 6.2). Results: we found no correlation between age and the estimated OD in our subjects (Spearman's correlation coefficient (r) = 0.123, one-tailed p value was 0.135). Patients with intermittent claudication, critical lower limb ischaemia, and aneurysms had higher estimated OD, and therefore higher anti-HSP70 antibody levels, than controls (Mann-Whitney test; p = 0.0127, 0.0037, 0.0008, respectively). Conclusions: our data provide the first evidence of a correlation between anti-HSP70 antibodies and different types of vascular diseases, suggesting that HSP70 might be involved in the pathogenesis and propagation of atherosclerosis. Since the immune response to HSPs can be modulated, this opens up the possibility of new therapeutic approaches.en_HK
dc.languageengen_US
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ejvsen_HK
dc.relation.ispartofEuropean Journal of Vascular and Endovascular Surgeryen_HK
dc.subjectAneurysmen_HK
dc.subjectAntibodiesen_HK
dc.subjectCritical ischaemiaen_HK
dc.subjectELISAen_HK
dc.subjectHeat-shock protein 70en_HK
dc.subjectIntermittent claudicationen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntibodies - Blooden_US
dc.subject.meshAortic Aneurysm, Abdominal - Immunologyen_US
dc.subject.meshCritical Illnessen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methods - Statistics & Numerical Dataen_US
dc.subject.meshFemaleen_US
dc.subject.meshHsp70 Heat-Shock Proteins - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIntermittent Claudication - Immunologyen_US
dc.subject.meshIschemia - Immunologyen_US
dc.subject.meshLeg - Blood Supplyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshReference Valuesen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.titleAnti-heat-shock protein 70 kDa antibodies in vascular patientsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, YC: ycchan88@hkucc.hku.hken_HK
dc.identifier.authorityChan, YC=rp00530en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1053/ejvs.1999.0885en_HK
dc.identifier.pmid10610825-
dc.identifier.scopuseid_2-s2.0-0033230175en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033230175&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue5en_HK
dc.identifier.spage381en_HK
dc.identifier.epage385en_HK
dc.identifier.isiWOS:000083996400003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, YC=27170769400en_HK
dc.identifier.scopusauthoridShukla, N=7102998160en_HK
dc.identifier.scopusauthoridAbdusSamee, M=6507802868en_HK
dc.identifier.scopusauthoridBerwanger, CS=6701673031en_HK
dc.identifier.scopusauthoridStanford, J=7202266598en_HK
dc.identifier.scopusauthoridSingh, M=35434195600en_HK
dc.identifier.scopusauthoridMansfield, AO=7005917823en_HK
dc.identifier.scopusauthoridStanby, G=6504426465en_HK

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