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Article: Culture of chondrocytes of hereditary multiple exostosis in vitro

TitleCulture of chondrocytes of hereditary multiple exostosis in vitro
Authors
Issue Date2003
Citation
Chinese Journal Of Clinical Rehabilitation, 2003, v. 7 n. 26, p. 3590-3591 How to Cite?
AbstractAim: To observe growth and related biological characters by culturing chondrocytes of hereditary multiple exostosis in vitro from human and to establish bases for further research on the etiology of hereditary multiple exostosis. Methods: Chondrocytes were culture primarily from exostosis and passaged. The morphology of chondrocytes was observed by microscope. Meanwhile the abilities of cell proliferation and adhesive rates were detected and were compared with control cartilage chondrocytes. Results: The living chondrocytes still were 95% after complete digestion. After passaged, exostosis chondrocytes grew in mololayer culture had a stellate appearance with more sticklike structures in the cytoplasm and had many obvious granules. Proliferation of exostosis chondrocytes turned slowly with passaged incresed, and the obvious difference existed between the 2th passaged and the primary exostosis chondrocytes( P < 0.01, t = 3.203) Most of the primary exostosis chondrocytes could adhere dishes within 24 hours. But with passaged incresed, the adhesive abilities turned down. The obvious difference existed between the 4th passaged and the primary exostosis chondrocytes. (P < 0.01, t = 4.611). Conclusion: The morphology and biological characters (proliferation, adhesive ability) of exostosis chondrocytes are different from control normal cartilage chondrocytes. It can establish some culture bases for further etiology research of hereditary multiple exostosis.
Persistent Identifierhttp://hdl.handle.net/10722/147520
ISSN
2009 SCImago Journal Rankings: 0.105
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Len_HK
dc.contributor.authorLuo, ZJen_HK
dc.contributor.authorJi, Qen_HK
dc.contributor.authorJin, XBen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorHui, WSen_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorCheah, KSEen_HK
dc.date.accessioned2012-05-29T06:04:19Z-
dc.date.available2012-05-29T06:04:19Z-
dc.date.issued2003en_HK
dc.identifier.citationChinese Journal Of Clinical Rehabilitation, 2003, v. 7 n. 26, p. 3590-3591en_HK
dc.identifier.issn1671-5926en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147520-
dc.description.abstractAim: To observe growth and related biological characters by culturing chondrocytes of hereditary multiple exostosis in vitro from human and to establish bases for further research on the etiology of hereditary multiple exostosis. Methods: Chondrocytes were culture primarily from exostosis and passaged. The morphology of chondrocytes was observed by microscope. Meanwhile the abilities of cell proliferation and adhesive rates were detected and were compared with control cartilage chondrocytes. Results: The living chondrocytes still were 95% after complete digestion. After passaged, exostosis chondrocytes grew in mololayer culture had a stellate appearance with more sticklike structures in the cytoplasm and had many obvious granules. Proliferation of exostosis chondrocytes turned slowly with passaged incresed, and the obvious difference existed between the 2th passaged and the primary exostosis chondrocytes( P < 0.01, t = 3.203) Most of the primary exostosis chondrocytes could adhere dishes within 24 hours. But with passaged incresed, the adhesive abilities turned down. The obvious difference existed between the 4th passaged and the primary exostosis chondrocytes. (P < 0.01, t = 4.611). Conclusion: The morphology and biological characters (proliferation, adhesive ability) of exostosis chondrocytes are different from control normal cartilage chondrocytes. It can establish some culture bases for further etiology research of hereditary multiple exostosis.en_HK
dc.languageengen_US
dc.relation.ispartofChinese Journal of Clinical Rehabilitationen_HK
dc.titleCulture of chondrocytes of hereditary multiple exostosis in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, D:chand@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-2442522420en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442522420&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue26en_HK
dc.identifier.spage3590en_HK
dc.identifier.epage3591en_HK
dc.identifier.scopusauthoridYang, L=9845703100en_HK
dc.identifier.scopusauthoridLuo, ZJ=8510080000en_HK
dc.identifier.scopusauthoridJi, Q=55186488000en_HK
dc.identifier.scopusauthoridJin, XB=55238349300en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridHui, WS=7103196473en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK

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