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Article: Pertussis toxin, but not tyrosine kinase inhibitors, abolishes effects of U-50488H on [Ca2+](i) in myocytes

TitlePertussis toxin, but not tyrosine kinase inhibitors, abolishes effects of U-50488H on [Ca2+](i) in myocytes
Authors
Issue Date1997
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal of Physiology - Cell Physiology, 1997, v. 272 n. 2 41-2, p. C560-C564 How to Cite?
AbstractThe effects of 10-5 M trans-3,4-dichloro-N-[2-(1- pyrrolidinyl)cyclohexyl]benzeacetamidel (U-50488H), a κ-opioid receptor agonist, on cytosolic Ca2+ concentration ([Ca2+](i)) and the [Ca2+](i) transient in quiescent and electrically stimulated rat ventricular myocytes, respectively, were determined after the cells had been pretreated with pertussis toxin (PTX) or a tyrosine kinase inhibitor (genistein or tyrphostin). The [Ca2+](i) was determined with a spectrofluorometric method, with fura 2 as Ca2+ indicator. U-50488H at 10-5 M itself induced a [Ca2+](i) transient in the quiescent cells but inhibited the [Ca2+](i) transient in electrically stimulated cells. The effects of 10-5 M U-50488H on [Ca2+](i), which were blocked by a selective K-opioid receptor antagonist, nor-binaltorphimine (10-6 M), were abolished after pretreatment with PTX (1 μg/ml) for 24 h, but not with genistein (10-4 M) or tyrphostin (5 x 10-5 M) for 30 min. 1-[6-[[(17b)-3-Methoxyestra-1,3,5(10)-trien-17- yl]amino]hexyl]-1H-pyrrole-2,5-dione (U-73122), an inhibitor of phospholipase C, at 10-5 M, but not its inactive structural isomer 1-[6-[[(17b)-3- methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-2,5-pyrrolidinedione (U- 73343), also blocked the Ca2+ responses to U-50488H. The results indicate that activation of phospholipase C on κ-opioid receptor stimulation is via PTX-sensitive G proteins but does not involve protein tyrosine phosphorylation.
Persistent Identifierhttp://hdl.handle.net/10722/147508
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSheng, JZen_US
dc.contributor.authorWong, NSen_US
dc.contributor.authorWang, HXen_US
dc.contributor.authorWong, TMen_US
dc.date.accessioned2012-05-29T06:04:13Z-
dc.date.available2012-05-29T06:04:13Z-
dc.date.issued1997en_US
dc.identifier.citationAmerican Journal of Physiology - Cell Physiology, 1997, v. 272 n. 2 41-2, p. C560-C564en_US
dc.identifier.issn0363-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/147508-
dc.description.abstractThe effects of 10-5 M trans-3,4-dichloro-N-[2-(1- pyrrolidinyl)cyclohexyl]benzeacetamidel (U-50488H), a κ-opioid receptor agonist, on cytosolic Ca2+ concentration ([Ca2+](i)) and the [Ca2+](i) transient in quiescent and electrically stimulated rat ventricular myocytes, respectively, were determined after the cells had been pretreated with pertussis toxin (PTX) or a tyrosine kinase inhibitor (genistein or tyrphostin). The [Ca2+](i) was determined with a spectrofluorometric method, with fura 2 as Ca2+ indicator. U-50488H at 10-5 M itself induced a [Ca2+](i) transient in the quiescent cells but inhibited the [Ca2+](i) transient in electrically stimulated cells. The effects of 10-5 M U-50488H on [Ca2+](i), which were blocked by a selective K-opioid receptor antagonist, nor-binaltorphimine (10-6 M), were abolished after pretreatment with PTX (1 μg/ml) for 24 h, but not with genistein (10-4 M) or tyrphostin (5 x 10-5 M) for 30 min. 1-[6-[[(17b)-3-Methoxyestra-1,3,5(10)-trien-17- yl]amino]hexyl]-1H-pyrrole-2,5-dione (U-73122), an inhibitor of phospholipase C, at 10-5 M, but not its inactive structural isomer 1-[6-[[(17b)-3- methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-2,5-pyrrolidinedione (U- 73343), also blocked the Ca2+ responses to U-50488H. The results indicate that activation of phospholipase C on κ-opioid receptor stimulation is via PTX-sensitive G proteins but does not involve protein tyrosine phosphorylation.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.mesh3,4-Dichloro-N-Methyl-N-(2-(1-Pyrrolidinyl)-Cyclohexyl)-Benzeneacetamide, (Trans)-Isomeren_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCell Separationen_US
dc.subject.meshCytosol - Metabolismen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshEstrenes - Pharmacologyen_US
dc.subject.meshHeart Ventriclesen_US
dc.subject.meshMaleen_US
dc.subject.meshMyocardium - Cytology - Metabolismen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshPertussis Toxinen_US
dc.subject.meshProtein-Tyrosine Kinases - Antagonists & Inhibitorsen_US
dc.subject.meshPyrrolidines - Antagonists & Inhibitors - Pharmacologyen_US
dc.subject.meshPyrrolidinones - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshVirulence Factors, Bordetella - Pharmacologyen_US
dc.titlePertussis toxin, but not tyrosine kinase inhibitors, abolishes effects of U-50488H on [Ca2+](i) in myocytesen_US
dc.typeArticleen_US
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_US
dc.identifier.authorityWong, NS=rp00340en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9124299-
dc.identifier.scopuseid_2-s2.0-16944367171en_US
dc.identifier.hkuros25881-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16944367171&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume272en_US
dc.identifier.issue2 41-2en_US
dc.identifier.spageC560en_US
dc.identifier.epageC564en_US
dc.identifier.isiWOS:A1997WJ63300023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSheng, JZ=36846286500en_US
dc.identifier.scopusauthoridWong, NS=7202836641en_US
dc.identifier.scopusauthoridWang, HX=15119990000en_US
dc.identifier.scopusauthoridWong, TM=7403531434en_US

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