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Article: Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs

TitleDeep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs
Authors
Issue Date2010
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2010, v. 116 n. 23, p. e118-e127 How to Cite?
AbstractA role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells through deep sequencing of 31 normal and malignant human B-cell samples that comprise the spectrum of B-cell differentiation and common malignant phenotypes. We identified the expression of 333 known miRNAs, which is more than twice the number previously recognized in any tissue type. We further identified the expression of 286 candidate novel miRNAs in normal and malignant B cells. These miRNAs were validated at a high rate (92%) using quantitative polymerase chain reaction, and we demonstrated their application in the distinction of clinically relevant subgroups of lymphoma. We further demonstrated that a novel miRNA cluster, previously annotated as a hypothetical gene LOC100130622, contains 6 novel miRNAs that regulate the transforming growth factor-β pathway. Thus, our work suggests that more than a third of the miRNAs present in most cellular types are currently unknown and that these miRNAs may regulate important cellular functions.
Persistent Identifierhttp://hdl.handle.net/10722/139441
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Doris Duke Charitable Foundation
National Institutes of Health
Funding Information:

S.S.D. was supported by the Doris Duke Charitable Foundation and the National Institutes of Health.

References

 

DC FieldValueLanguage
dc.contributor.authorJima, DDen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorJacobs, Cen_HK
dc.contributor.authorRichards, KLen_HK
dc.contributor.authorDunphy, CHen_HK
dc.contributor.authorChoi, WWLen_HK
dc.contributor.authorAu, WYen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorCzader, MBen_HK
dc.contributor.authorRizzieri, DAen_HK
dc.contributor.authorLagoo, ASen_HK
dc.contributor.authorLugar, PLen_HK
dc.contributor.authorMann, KPen_HK
dc.contributor.authorFlowers, CRen_HK
dc.contributor.authorBernalMizrachi, Len_HK
dc.contributor.authorNaresh, KNen_HK
dc.contributor.authorEvens, AMen_HK
dc.contributor.authorGordon, LIen_HK
dc.contributor.authorLuftig, Men_HK
dc.contributor.authorFriedman, DRen_HK
dc.contributor.authorWeinberg, JBen_HK
dc.contributor.authorThompson, MAen_HK
dc.contributor.authorGill, JIen_HK
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorHow, Ten_HK
dc.contributor.authorGrubor, Ven_HK
dc.contributor.authorGao, Yen_HK
dc.contributor.authorPatel, Aen_HK
dc.contributor.authorWu, Hen_HK
dc.contributor.authorZhu, Jen_HK
dc.contributor.authorBlobe, GCen_HK
dc.contributor.authorLipsky, PEen_HK
dc.contributor.authorChadburn, Aen_HK
dc.contributor.authorDave, SSen_HK
dc.date.accessioned2011-09-23T05:49:51Z-
dc.date.available2011-09-23T05:49:51Z-
dc.date.issued2010en_HK
dc.identifier.citationBlood, 2010, v. 116 n. 23, p. e118-e127en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139441-
dc.description.abstractA role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells through deep sequencing of 31 normal and malignant human B-cell samples that comprise the spectrum of B-cell differentiation and common malignant phenotypes. We identified the expression of 333 known miRNAs, which is more than twice the number previously recognized in any tissue type. We further identified the expression of 286 candidate novel miRNAs in normal and malignant B cells. These miRNAs were validated at a high rate (92%) using quantitative polymerase chain reaction, and we demonstrated their application in the distinction of clinically relevant subgroups of lymphoma. We further demonstrated that a novel miRNA cluster, previously annotated as a hypothetical gene LOC100130622, contains 6 novel miRNAs that regulate the transforming growth factor-β pathway. Thus, our work suggests that more than a third of the miRNAs present in most cellular types are currently unknown and that these miRNAs may regulate important cellular functions.en_HK
dc.languageengen_US
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.rightsThis research was originally published in The Hematologist: ASH News and Reports. Author(s). Title. The Hematologist: ASH News and Reports. Year;Vol,Issue:pp-pp. © the American Society of Hematology.en_US
dc.subject.meshB-Lymphocytes-
dc.subject.meshGene Expression Profiling - methods-
dc.subject.meshLymphoma, Large B-Cell, Diffuse - genetics-
dc.subject.meshMicroRNAs - analysis - genetics-
dc.titleDeep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=116&issue=23&spage=e118&epage=e127&date=2010&atitle=Deep+sequencing+of+the+small+RNA+transcriptome+of+normal+and+malignant+human+B+cells+identifies+hundreds+of+novel+microRNAsen_US
dc.identifier.emailChoi, WWL:wlchoi@pathology.hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityChoi, WWL=rp00247en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1182/blood-2010-05-285403en_HK
dc.identifier.pmid20733160-
dc.identifier.pmcidPMC3012600-
dc.identifier.scopuseid_2-s2.0-78649743127en_HK
dc.identifier.hkuros183788en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649743127&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume116en_HK
dc.identifier.issue23en_HK
dc.identifier.spagee118en_HK
dc.identifier.epagee127en_HK
dc.identifier.eissn1528-0020-
dc.identifier.isiWOS:000284880200001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike9054399-

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