Article: Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract

TitleTropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract
Authors
Issue Date2010
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 2010, v. 176 n. 4, p. 1828-1840 How to Cite?
AbstractThe novel pandemic influenza H1N1 (H1N1pdm) virus of swine origin causes mild disease but occasionally leads to acute respiratory distress syndrome and death. It is important to understand the pathogenesis of this new disease in humans. We compared the virus tropism and host-responses elicited by pandemic H1N1pdm and seasonal H1N1 influenza viruses in ex vivo cultures of human conjunctiva, nasopharynx, bronchus, and lung, as well as in vitro cultures of human nasopharyngeal, bronchial, and alveolar epithelial cells. We found comparable replication and host-responses in seasonal and pandemic H1N1 viruses. However, pandemic H1N1pdm virus differs from seasonal H1N1 influenza virus in its ability to replicate in human conjunctiva, suggesting subtle differences in its receptor-binding profile and highlighting the potential role of the conjunctiva as an additional route of infection with H1N1pdm. A greater viral replication competence in bronchial epithelium at 33°C may also contribute to the slight increase in virulence of the pandemic influenza virus. In contrast with highly pathogenic influenza H5N1 virus, pandemic H1N1pdm does not differ from seasonal influenza virus in its intrinsic capacity for cytokine dysregulation. Collectively, these results suggest that pandemic H1N1pdm virus differs in modest but subtle ways from seasonal H1N1 virus in its intrinsic virulence for humans, which is in accord with the epidemiology of the pandemic to date. These findings are therefore relevant for understanding transmission and therapy. Copyright © American Society for Investigative Pathology.
Persistent Identifierhttp://hdl.handle.net/10722/138946
ISSN
2014 Impact Factor: 4.591
2014 SCImago Journal Rankings: 2.259
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Fund for Control of Infectious Disease06060552
08070842
Research Fund for Control of Infectious Disease, Health, Welfare, and Food Bureau, Hong Kong SAR Government
General Research Fund (HKU)7612/08M
7610/09M
7530/06M
7735/07M
Research Grants Council, Hong Kong SAR Government200907176007
The University of Hong Kong
National Institutes of Health (NIAID)HHSN266200700005C
Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
Funding Information:

Supported by Research Fund for Control of Infectious Disease grant (Ref: LAB-15, RFCID commissioned study on human swine influenza virus and RFCID grant, reference no: 06060552, 08070842) from the Research Fund for Control of Infectious Disease, Health, Welfare, and Food Bureau, Hong Kong SAR Government, and the General Research Fund (HKU 7612/08M and 7610/09M to M.C.W.C., HKU 7530/06M to L.L.M.P and HKU 7735/07M to J.M.N), Research Grants Council, Hong Kong SAR Government; Small project funding (reference no: 200907176007 to R.W.Y.C), The University of Hong Kong; National Institutes of Health (NIAID contract HHSN266200700005C) and AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorChan, MCWen_HK
dc.contributor.authorChan, RWYen_HK
dc.contributor.authorYu, WCLen_HK
dc.contributor.authorHo, CCCen_HK
dc.contributor.authorYuen, KMen_HK
dc.contributor.authorFong, JHMen_HK
dc.contributor.authorTang, LLSen_HK
dc.contributor.authorLai, WWen_HK
dc.contributor.authorLo, ACYen_HK
dc.contributor.authorChui, WHen_HK
dc.contributor.authorSihoe, ADLen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorWong, DSHen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2011-09-23T05:42:39Z-
dc.date.available2011-09-23T05:42:39Z-
dc.date.issued2010en_HK
dc.identifier.citationAmerican Journal Of Pathology, 2010, v. 176 n. 4, p. 1828-1840en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138946-
dc.description.abstractThe novel pandemic influenza H1N1 (H1N1pdm) virus of swine origin causes mild disease but occasionally leads to acute respiratory distress syndrome and death. It is important to understand the pathogenesis of this new disease in humans. We compared the virus tropism and host-responses elicited by pandemic H1N1pdm and seasonal H1N1 influenza viruses in ex vivo cultures of human conjunctiva, nasopharynx, bronchus, and lung, as well as in vitro cultures of human nasopharyngeal, bronchial, and alveolar epithelial cells. We found comparable replication and host-responses in seasonal and pandemic H1N1 viruses. However, pandemic H1N1pdm virus differs from seasonal H1N1 influenza virus in its ability to replicate in human conjunctiva, suggesting subtle differences in its receptor-binding profile and highlighting the potential role of the conjunctiva as an additional route of infection with H1N1pdm. A greater viral replication competence in bronchial epithelium at 33°C may also contribute to the slight increase in virulence of the pandemic influenza virus. In contrast with highly pathogenic influenza H5N1 virus, pandemic H1N1pdm does not differ from seasonal influenza virus in its intrinsic capacity for cytokine dysregulation. Collectively, these results suggest that pandemic H1N1pdm virus differs in modest but subtle ways from seasonal H1N1 virus in its intrinsic virulence for humans, which is in accord with the epidemiology of the pandemic to date. These findings are therefore relevant for understanding transmission and therapy. Copyright © American Society for Investigative Pathology.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.subject.meshBronchi - cytology-
dc.subject.meshConjunctiva - virology-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - metabolism-
dc.subject.meshInfluenza, Human - virology-
dc.subject.meshRespiratory System - virology-
dc.titleTropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tracten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9440&volume=176&issue=4&spage=1828&epage=1840&date=2010&atitle=Tropism+and+innate+host+responses+of+the+2009+pandemic+H1N1+influenza+virus+in+ex+vivo+and+in+vitro+cultures+of+human+conjunctiva+and+respiratory+tract-
dc.identifier.emailChan, MCW: mchan@hku.hken_HK
dc.identifier.emailChan, RWY: reneewy@hku.hken_HK
dc.identifier.emailLai, WW: wicolai@hku.hken_HK
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_HK
dc.identifier.emailKwong, DLW: dlwkwong@hku.hken_HK
dc.identifier.emailWong, DSH: shdwong@hku.hken_HK
dc.identifier.emailTsao, GSW: gswtsao@hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityChan, MCW=rp00420en_HK
dc.identifier.authorityChan, RWY=rp01596en_HK
dc.identifier.authorityLai, WW=rp00531en_HK
dc.identifier.authorityLo, ACY=rp00425en_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityWong, DSH=rp00516en_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.2353/ajpath.2010.091087en_HK
dc.identifier.pmid20110407en_HK
dc.identifier.pmcidPMC2843473-
dc.identifier.scopuseid_2-s2.0-77950584589en_HK
dc.identifier.hkuros172048en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950584589&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume176en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1828en_HK
dc.identifier.epage1840en_HK
dc.identifier.eissn1525-2191-
dc.identifier.isiWOS:000276471500029-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectReplication and pathogenesis of avian influenza A (H5N1) viruses in polarized human bronchial and alveolar epithelium-
dc.relation.projectSusceptibility of the upper respiratory tract to influenza virus infection following desialyation-
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridChan, MCW=26654715500en_HK
dc.identifier.scopusauthoridChan, RWY=26661379100en_HK
dc.identifier.scopusauthoridYu, WCL=26324133100en_HK
dc.identifier.scopusauthoridHo, CCC=35299371300en_HK
dc.identifier.scopusauthoridYuen, KM=35301205900en_HK
dc.identifier.scopusauthoridFong, JHM=36790906900en_HK
dc.identifier.scopusauthoridTang, LLS=36999571500en_HK
dc.identifier.scopusauthoridLai, WW=7402231098en_HK
dc.identifier.scopusauthoridLo, ACY=7102780640en_HK
dc.identifier.scopusauthoridChui, WH=7003524497en_HK
dc.identifier.scopusauthoridSihoe, ADL=6603611976en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridWong, DSH=7401536078en_HK
dc.identifier.scopusauthoridTsao, GSW=7102813116en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridNicholls, JM=7201463077en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK

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