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Article: Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract
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TitleTropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract
 
AuthorsChan, MCW1
Chan, RWY1
Yu, WCL1
Ho, CCC1
Yuen, KM1
Fong, JHM1
Tang, LLS1
Lai, WW1
Lo, ACY1
Chui, WH1
Sihoe, ADL1
Kwong, DLW1
Wong, DSH1
Tsao, GSW1
Poon, LLM1
Guan, Y1
Nicholls, JM1
Peiris, JSM2
 
Issue Date2010
 
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
 
CitationAmerican Journal Of Pathology, 2010, v. 176 n. 4, p. 1828-1840 [How to Cite?]
DOI: http://dx.doi.org/10.2353/ajpath.2010.091087
 
AbstractThe novel pandemic influenza H1N1 (H1N1pdm) virus of swine origin causes mild disease but occasionally leads to acute respiratory distress syndrome and death. It is important to understand the pathogenesis of this new disease in humans. We compared the virus tropism and host-responses elicited by pandemic H1N1pdm and seasonal H1N1 influenza viruses in ex vivo cultures of human conjunctiva, nasopharynx, bronchus, and lung, as well as in vitro cultures of human nasopharyngeal, bronchial, and alveolar epithelial cells. We found comparable replication and host-responses in seasonal and pandemic H1N1 viruses. However, pandemic H1N1pdm virus differs from seasonal H1N1 influenza virus in its ability to replicate in human conjunctiva, suggesting subtle differences in its receptor-binding profile and highlighting the potential role of the conjunctiva as an additional route of infection with H1N1pdm. A greater viral replication competence in bronchial epithelium at 33°C may also contribute to the slight increase in virulence of the pandemic influenza virus. In contrast with highly pathogenic influenza H5N1 virus, pandemic H1N1pdm does not differ from seasonal influenza virus in its intrinsic capacity for cytokine dysregulation. Collectively, these results suggest that pandemic H1N1pdm virus differs in modest but subtle ways from seasonal H1N1 virus in its intrinsic virulence for humans, which is in accord with the epidemiology of the pandemic to date. These findings are therefore relevant for understanding transmission and therapy. Copyright © American Society for Investigative Pathology.
 
ISSN0002-9440
2013 Impact Factor: 4.602
 
DOIhttp://dx.doi.org/10.2353/ajpath.2010.091087
 
PubMed Central IDPMC2843473
 
ISI Accession Number IDWOS:000276471500029
Funding AgencyGrant Number
Research Fund for Control of Infectious Disease06060552
08070842
Research Fund for Control of Infectious Disease, Health, Welfare, and Food Bureau, Hong Kong SAR Government
General Research Fund (HKU)7612/08M
7610/09M
7530/06M
7735/07M
Research Grants Council, Hong Kong SAR Government200907176007
The University of Hong Kong
National Institutes of Health (NIAID)HHSN266200700005C
Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
Funding Information:

Supported by Research Fund for Control of Infectious Disease grant (Ref: LAB-15, RFCID commissioned study on human swine influenza virus and RFCID grant, reference no: 06060552, 08070842) from the Research Fund for Control of Infectious Disease, Health, Welfare, and Food Bureau, Hong Kong SAR Government, and the General Research Fund (HKU 7612/08M and 7610/09M to M.C.W.C., HKU 7530/06M to L.L.M.P and HKU 7735/07M to J.M.N), Research Grants Council, Hong Kong SAR Government; Small project funding (reference no: 200907176007 to R.W.Y.C), The University of Hong Kong; National Institutes of Health (NIAID contract HHSN266200700005C) and AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government.

 
ReferencesReferences in Scopus
 
GrantsReplication and pathogenesis of avian influenza A (H5N1) viruses in polarized human bronchial and alveolar epithelium
Susceptibility of the upper respiratory tract to influenza virus infection following desialyation
Control of Pandemic and Inter-pandemic Influenza
 
DC FieldValue
dc.contributor.authorChan, MCW
 
dc.contributor.authorChan, RWY
 
dc.contributor.authorYu, WCL
 
dc.contributor.authorHo, CCC
 
dc.contributor.authorYuen, KM
 
dc.contributor.authorFong, JHM
 
dc.contributor.authorTang, LLS
 
dc.contributor.authorLai, WW
 
dc.contributor.authorLo, ACY
 
dc.contributor.authorChui, WH
 
dc.contributor.authorSihoe, ADL
 
dc.contributor.authorKwong, DLW
 
dc.contributor.authorWong, DSH
 
dc.contributor.authorTsao, GSW
 
dc.contributor.authorPoon, LLM
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorNicholls, JM
 
dc.contributor.authorPeiris, JSM
 
dc.date.accessioned2011-09-23T05:42:39Z
 
dc.date.available2011-09-23T05:42:39Z
 
dc.date.issued2010
 
dc.description.abstractThe novel pandemic influenza H1N1 (H1N1pdm) virus of swine origin causes mild disease but occasionally leads to acute respiratory distress syndrome and death. It is important to understand the pathogenesis of this new disease in humans. We compared the virus tropism and host-responses elicited by pandemic H1N1pdm and seasonal H1N1 influenza viruses in ex vivo cultures of human conjunctiva, nasopharynx, bronchus, and lung, as well as in vitro cultures of human nasopharyngeal, bronchial, and alveolar epithelial cells. We found comparable replication and host-responses in seasonal and pandemic H1N1 viruses. However, pandemic H1N1pdm virus differs from seasonal H1N1 influenza virus in its ability to replicate in human conjunctiva, suggesting subtle differences in its receptor-binding profile and highlighting the potential role of the conjunctiva as an additional route of infection with H1N1pdm. A greater viral replication competence in bronchial epithelium at 33°C may also contribute to the slight increase in virulence of the pandemic influenza virus. In contrast with highly pathogenic influenza H5N1 virus, pandemic H1N1pdm does not differ from seasonal influenza virus in its intrinsic capacity for cytokine dysregulation. Collectively, these results suggest that pandemic H1N1pdm virus differs in modest but subtle ways from seasonal H1N1 virus in its intrinsic virulence for humans, which is in accord with the epidemiology of the pandemic to date. These findings are therefore relevant for understanding transmission and therapy. Copyright © American Society for Investigative Pathology.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationAmerican Journal Of Pathology, 2010, v. 176 n. 4, p. 1828-1840 [How to Cite?]
DOI: http://dx.doi.org/10.2353/ajpath.2010.091087
 
dc.identifier.doihttp://dx.doi.org/10.2353/ajpath.2010.091087
 
dc.identifier.eissn1525-2191
 
dc.identifier.epage1840
 
dc.identifier.hkuros172048
 
dc.identifier.isiWOS:000276471500029
Funding AgencyGrant Number
Research Fund for Control of Infectious Disease06060552
08070842
Research Fund for Control of Infectious Disease, Health, Welfare, and Food Bureau, Hong Kong SAR Government
General Research Fund (HKU)7612/08M
7610/09M
7530/06M
7735/07M
Research Grants Council, Hong Kong SAR Government200907176007
The University of Hong Kong
National Institutes of Health (NIAID)HHSN266200700005C
Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
Funding Information:

Supported by Research Fund for Control of Infectious Disease grant (Ref: LAB-15, RFCID commissioned study on human swine influenza virus and RFCID grant, reference no: 06060552, 08070842) from the Research Fund for Control of Infectious Disease, Health, Welfare, and Food Bureau, Hong Kong SAR Government, and the General Research Fund (HKU 7612/08M and 7610/09M to M.C.W.C., HKU 7530/06M to L.L.M.P and HKU 7735/07M to J.M.N), Research Grants Council, Hong Kong SAR Government; Small project funding (reference no: 200907176007 to R.W.Y.C), The University of Hong Kong; National Institutes of Health (NIAID contract HHSN266200700005C) and AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government.

 
dc.identifier.issn0002-9440
2013 Impact Factor: 4.602
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC2843473
 
dc.identifier.pmid20110407
 
dc.identifier.scopuseid_2-s2.0-77950584589
 
dc.identifier.spage1828
 
dc.identifier.urihttp://hdl.handle.net/10722/138946
 
dc.identifier.volume176
 
dc.languageeng
 
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Pathology
 
dc.relation.projectReplication and pathogenesis of avian influenza A (H5N1) viruses in polarized human bronchial and alveolar epithelium
 
dc.relation.projectSusceptibility of the upper respiratory tract to influenza virus infection following desialyation
 
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBronchi - cytology
 
dc.subject.meshConjunctiva - virology
 
dc.subject.meshInfluenza A Virus, H1N1 Subtype - metabolism
 
dc.subject.meshInfluenza, Human - virology
 
dc.subject.meshRespiratory System - virology
 
dc.titleTropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. HKU-Pasteur Research Centre