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Conference Paper: Functional characterization of Dihydroflavonol 4-reductase-like1 (DRL1) homologs in plant male gametophyte development

TitleFunctional characterization of Dihydroflavonol 4-reductase-like1 (DRL1) homologs in plant male gametophyte development
Authors
Issue Date2010
PublisherAmerican Society of Plant Biologists and the Canadian Society of Plant Physiologists.
Citation
Plant Biology 2010, Joint Annual Meetings of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists, Montreal, Canada, 31 July- 4 August 2010, p. abstract no. P04054 How to Cite?
AbstractThe process of pollen development in higher plants is a complex strategy involving a diverse range of genes and interactions of gene products. However, little is known about the genes involved in the biosynthesis of the phenolic constituents of pollen wall. Previously, we have reported an anther-specific DRL1 (Dihydroflavonol 4-reductase-like1) gene in Arabidopsis, which is essential for pollen development and male fertility. However, its role in pollen development has not been fully characterized. Using the native promoter and a functional enhanced yellow fluorescent protein fusion to analyze the temporal and spatial expression of AtDRL1, we demonstrated that the DRL1: eYFP fusion protein is localized within the tapetum and is expressed in a developmentally regulated manner during early microsporogenesis. Moreover, the expression of the DRL1: eYFP fusion protein in drl1 homozygous mutants can complement the phenotype and restore seed formation. Another DRL1 homolog from tobacco was designated NtDRL1. Its full coding sequence and promoter region were isolated by RT-PCR and TAIL-PCR respectively. NtDRL1 expression was only detected in the developing tobacco anther, especially in stage -1 to 2, which corresponding to the meiosis and young microspore stage. In situ hybridization analysis showed NtDRL1 was expressed only in the tapetum layer of anther. Based on the analysis of the gene and protein expression profiles, DRL1 homolog genes are finely regulated during anther development and play a critical role in production of viable pollen. However, the detailed biochemical functions of DRL1 homologs remain unknown. Due to the high sequence homology, DRL1 homologs may be involved in a common biosynthesis pathway of phenolic compounds.
DescriptionConference theme: Bringing together the global community of plant biologists
Poster session: P04 - Development
Persistent Identifierhttp://hdl.handle.net/10722/138282

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_US
dc.contributor.authorLo, CSCen_US
dc.date.accessioned2011-08-26T14:44:19Z-
dc.date.available2011-08-26T14:44:19Z-
dc.date.issued2010en_US
dc.identifier.citationPlant Biology 2010, Joint Annual Meetings of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists, Montreal, Canada, 31 July- 4 August 2010, p. abstract no. P04054en_US
dc.identifier.urihttp://hdl.handle.net/10722/138282-
dc.descriptionConference theme: Bringing together the global community of plant biologists-
dc.descriptionPoster session: P04 - Development-
dc.description.abstractThe process of pollen development in higher plants is a complex strategy involving a diverse range of genes and interactions of gene products. However, little is known about the genes involved in the biosynthesis of the phenolic constituents of pollen wall. Previously, we have reported an anther-specific DRL1 (Dihydroflavonol 4-reductase-like1) gene in Arabidopsis, which is essential for pollen development and male fertility. However, its role in pollen development has not been fully characterized. Using the native promoter and a functional enhanced yellow fluorescent protein fusion to analyze the temporal and spatial expression of AtDRL1, we demonstrated that the DRL1: eYFP fusion protein is localized within the tapetum and is expressed in a developmentally regulated manner during early microsporogenesis. Moreover, the expression of the DRL1: eYFP fusion protein in drl1 homozygous mutants can complement the phenotype and restore seed formation. Another DRL1 homolog from tobacco was designated NtDRL1. Its full coding sequence and promoter region were isolated by RT-PCR and TAIL-PCR respectively. NtDRL1 expression was only detected in the developing tobacco anther, especially in stage -1 to 2, which corresponding to the meiosis and young microspore stage. In situ hybridization analysis showed NtDRL1 was expressed only in the tapetum layer of anther. Based on the analysis of the gene and protein expression profiles, DRL1 homolog genes are finely regulated during anther development and play a critical role in production of viable pollen. However, the detailed biochemical functions of DRL1 homologs remain unknown. Due to the high sequence homology, DRL1 homologs may be involved in a common biosynthesis pathway of phenolic compounds.-
dc.languageengen_US
dc.publisherAmerican Society of Plant Biologists and the Canadian Society of Plant Physiologists.-
dc.relation.ispartofPlant Biology 2011: Annual Meeting of The American Society of Plant Biologists and the Canadian Society of Plant Physiologistsen_US
dc.titleFunctional characterization of Dihydroflavonol 4-reductase-like1 (DRL1) homologs in plant male gametophyte developmenten_US
dc.typeConference_Paperen_US
dc.identifier.emailLo, CSC: clivelo@hkucc.hku.hken_US
dc.identifier.authorityLo, CSC=rp00751en_US
dc.identifier.hkuros189522en_US
dc.identifier.spageabstract no. P04054-
dc.identifier.epageabstract no. P04054-
dc.publisher.placeUnited States-

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