File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Occurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across china and other global locations

TitleOccurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across china and other global locations
Authors
Issue Date2011
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2011, v. 45 n. 7, p. 2598-2604 How to Cite?
AbstractActivated sludge was sampled from 15 sewage treatment plants (STPs) across China and other global locations to investigate the occurrence, abundance and diversity of tetracycline resistance genes (tet) in the STPs. Occurrence and abundance of 14 tet genes were determined using polymerase chain reaction (PCR) and quantitative real time PCR. Six genes (tet(A), tet(C), tet(G), tet(M), tet(S), and tet(X)) were detected in all the STPs, while no sludge sample contained tet(Q). Total concentration of the 14 genes was significantly different among the STPs and average tet abundance of the STPs varied greatly among the tet types (p < 0.05). Tet(G) had the highest concentration in the STPs, followed by tet(C), tet(A) and tet(S). Phylogenetic diversity of the genes was investigated using DNA cloning. BLAST analysis showed that all of the 450 cloned sequences matched known tet genes, except for tet(G). The 56 tet(G) clones were grouped into 14 genotypes, among which type G24 had an identical sequence to tet(G) carried by Salmonella enterica or Acinetobacter baumannii, while the other sequences had low similarity to the known genes in GenBank. The results of this study might be useful to understand the diversity of these resistance genes in STPs. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/137227
ISSN
2015 Impact Factor: 5.393
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU7202/09E
Small Project Fund201007176069
University of Hong Kong
Funding Information:

This study was financially supported by the Research Grants Council of Hong Kong (HKU7202/09E), Small Project Fund (201007176069), and SPACE Research Fund of the University of Hong Kong. We would like to thank Prof. Gao DW, Prof. Deng BL, Dr. Huang QC, Dr. Zhu HG, Dr. Liang DW, Dr. Duan JZ, Dr. Zhang M, Mr. Yu K, Miss Yang Y, and Mr. Ye L for the sludge sampling.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, XXen_HK
dc.contributor.authorZhang, Ten_HK
dc.date.accessioned2011-08-26T14:21:35Z-
dc.date.available2011-08-26T14:21:35Z-
dc.date.issued2011en_HK
dc.identifier.citationEnvironmental Science And Technology, 2011, v. 45 n. 7, p. 2598-2604en_HK
dc.identifier.issn0013-936Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/137227-
dc.description.abstractActivated sludge was sampled from 15 sewage treatment plants (STPs) across China and other global locations to investigate the occurrence, abundance and diversity of tetracycline resistance genes (tet) in the STPs. Occurrence and abundance of 14 tet genes were determined using polymerase chain reaction (PCR) and quantitative real time PCR. Six genes (tet(A), tet(C), tet(G), tet(M), tet(S), and tet(X)) were detected in all the STPs, while no sludge sample contained tet(Q). Total concentration of the 14 genes was significantly different among the STPs and average tet abundance of the STPs varied greatly among the tet types (p < 0.05). Tet(G) had the highest concentration in the STPs, followed by tet(C), tet(A) and tet(S). Phylogenetic diversity of the genes was investigated using DNA cloning. BLAST analysis showed that all of the 450 cloned sequences matched known tet genes, except for tet(G). The 56 tet(G) clones were grouped into 14 genotypes, among which type G24 had an identical sequence to tet(G) carried by Salmonella enterica or Acinetobacter baumannii, while the other sequences had low similarity to the known genes in GenBank. The results of this study might be useful to understand the diversity of these resistance genes in STPs. © 2011 American Chemical Society.en_HK
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_HK
dc.relation.ispartofEnvironmental Science and Technologyen_HK
dc.subject.meshBacteria - classification - genetics - isolation and purification-
dc.subject.meshEnvironmental Monitoring-
dc.subject.meshSewage - microbiology-
dc.subject.meshTetracycline Resistance - genetics-
dc.subject.meshWaste Disposal, Fluid-
dc.titleOccurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across china and other global locationsen_HK
dc.typeArticleen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/es103672xen_HK
dc.identifier.pmid21388174-
dc.identifier.scopuseid_2-s2.0-79953240174en_HK
dc.identifier.hkuros190128en_US
dc.identifier.hkuros208074-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79953240174&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2598en_HK
dc.identifier.epage2604en_HK
dc.identifier.isiWOS:000288841500017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, XX=35239161000en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats