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Article: Homologous and heterologous enhancers modulate spatial expression but not cell-type specificity of the murine γF-crystallin promoter

TitleHomologous and heterologous enhancers modulate spatial expression but not cell-type specificity of the murine γF-crystallin promoter
Authors
Keywordsγ-crystallin
Developmental regulation
lacZ
Lens
Transgenic mice
Issue Date1990
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
Citation
Development, 1990, v. 110 n. 1, p. 131-139 How to Cite?
AbstractPrevious studies have shown that mouse γF-crystallin sequences -759 to +45, which include the core promoter and two upstream enhancer elements, contain sufficient information for directing gene expression to terminally differentiated fiber cells of the ocular lens. To investigate the role that proximal sequences of the mouse γF-crystallin promoter play in the developmental regulation of gene expression, we generated transgenic mice containing the lacZ gene driven by either mouse γF-crystallin sequences -171 to +45, which lack functional enhancers, or a hybrid hamster αA-/mouse γF-crystallin promoter, which contains the hamster αA-crystallin enhancer instead of operational γF-crystallin enhancers. In situ analysis of lacZ expression in these mice revealed that the mouse γF-crystallin promoter segment -171 to +45, which shows low activity in vitro, is able to direct gene expression to the fiber cells in the nucleus of the lens. However, animals expressing γ171-lacZ show both a lower level of expression of the lacZ gene and a narrower pattern of staining in the lens nucleus than mice expressing γ759-lacZ, which contains the two enhancer elements located between -392 and -278 and -226 to -123. Moreover, animals expressing the lacZ gene driven by the hybrid αA-/γF-crystallin promoter show a pattern of staining in the lens nucleus similar to that of mice expressing γ759-lacZ, indicating that although the γA-crystallin enhancer is unable to alter the cell-type specificity conferred by the mouse γF-crystallin proximal promoter segment -171 to +45, it is able to expand the spatial domain of gene expression in a manner similar to the native γF-crystallin enhancers. The significance of these results, in terms of the mechanisms governing spatial regulation of gene expression, is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/133883
ISSN
2015 Impact Factor: 6.059
2015 SCImago Journal Rankings: 5.239
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, CCKen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBreitman, MLen_HK
dc.date.accessioned2011-06-03T03:52:26Z-
dc.date.available2011-06-03T03:52:26Z-
dc.date.issued1990en_HK
dc.identifier.citationDevelopment, 1990, v. 110 n. 1, p. 131-139en_HK
dc.identifier.issn0950-1991en_HK
dc.identifier.urihttp://hdl.handle.net/10722/133883-
dc.description.abstractPrevious studies have shown that mouse γF-crystallin sequences -759 to +45, which include the core promoter and two upstream enhancer elements, contain sufficient information for directing gene expression to terminally differentiated fiber cells of the ocular lens. To investigate the role that proximal sequences of the mouse γF-crystallin promoter play in the developmental regulation of gene expression, we generated transgenic mice containing the lacZ gene driven by either mouse γF-crystallin sequences -171 to +45, which lack functional enhancers, or a hybrid hamster αA-/mouse γF-crystallin promoter, which contains the hamster αA-crystallin enhancer instead of operational γF-crystallin enhancers. In situ analysis of lacZ expression in these mice revealed that the mouse γF-crystallin promoter segment -171 to +45, which shows low activity in vitro, is able to direct gene expression to the fiber cells in the nucleus of the lens. However, animals expressing γ171-lacZ show both a lower level of expression of the lacZ gene and a narrower pattern of staining in the lens nucleus than mice expressing γ759-lacZ, which contains the two enhancer elements located between -392 and -278 and -226 to -123. Moreover, animals expressing the lacZ gene driven by the hybrid αA-/γF-crystallin promoter show a pattern of staining in the lens nucleus similar to that of mice expressing γ759-lacZ, indicating that although the γA-crystallin enhancer is unable to alter the cell-type specificity conferred by the mouse γF-crystallin proximal promoter segment -171 to +45, it is able to expand the spatial domain of gene expression in a manner similar to the native γF-crystallin enhancers. The significance of these results, in terms of the mechanisms governing spatial regulation of gene expression, is discussed.en_HK
dc.languageeng-
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.orgen_HK
dc.relation.ispartofDevelopmenten_HK
dc.subjectγ-crystallinen_HK
dc.subjectDevelopmental regulationen_HK
dc.subjectlacZen_HK
dc.subjectLensen_HK
dc.subjectTransgenic miceen_HK
dc.subject.meshCrystallins - genetics-
dc.subject.meshEnhancer Elements, Genetic - genetics-
dc.subject.meshGene Expression Regulation - genetics-
dc.subject.meshLens, Crystalline - embryology-
dc.subject.meshPromoter Regions, Genetic - genetics-
dc.titleHomologous and heterologous enhancers modulate spatial expression but not cell-type specificity of the murine γF-crystallin promoteren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-1991&volume=110&issue=1&spage=131&epage=139&date=1990&atitle=Homologous+and+heterologous+enhancers+modulate+spatial+expression+but+not+cell-type+specificity+of+the+murine+gamma+F-crystallin+promoter-
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid2081455-
dc.identifier.scopuseid_2-s2.0-0025180204en_HK
dc.identifier.volume110en_HK
dc.identifier.issue1en_HK
dc.identifier.spage131en_HK
dc.identifier.epage139en_HK
dc.identifier.isiWOS:A1990DZ67200012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYu, CCK=8071135400en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK

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