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Article: Upregulation of erythropoietin and its receptor expression in the rat carotid body during chronic and intermittent hypoxia

TitleUpregulation of erythropoietin and its receptor expression in the rat carotid body during chronic and intermittent hypoxia
Authors
KeywordsCarotid body
Chronic hypoxia
Erythropoietin
Intermittent hypoxia
Issue Date2009
PublisherSpringer New York LLC.
Citation
Advances In Experimental Medicine And Biology, 2009, v. 648, p. 207-214 How to Cite?
AbstractThe carotid body (CB) plays important roles in cardiorespiratory changes in intermittent hypoxia (IH). Erythropoietin (EPO), a hypoxia-inducible factor (HIF)-1 target gene, is present in the chemoreceptive type-I cells in the CB but its expression and role in IH resembling sleep apnoeic conditions are not known. We hypothesized that IH upregulates the expression of EPO and its receptor (EPOr) in the rat CB. The CB expressions of EPO and EPOr were examined in rats breathing 10% O 2 (in isobaric chamber for CH, 24 hour/day) or in IH (cyclic between air and 5% O 2 per minute, 8 hour/day) for 3-28 days. Immunohistochemical studies revealed that the EPO and EPOr proteins were localized in CB glomic clusters. The proportional amount of cells with positive staining of EPO immunoreactivities was significantly increased in both IH and CH groups when compared with the normoxic control. The EPO expression was more markedly increased in the CH than that of the IH groups throughout the time course, reaching a peak level at day 14. The positive EPOr immunostaining was increased significantly in the 3-day CH group. By day 14, the EPOr expression elevated considerably at peak levels in both IH and CH rats, whereas the elevation was greater in the CH rats. These results suggest an upregulation of EPO and its receptor expression in the rat CB under IH and CH conditions, presumably mediated by the activation of HIF-1 pathway. The increased EPO binding to its receptor might play a role in the enhancement of CB excitability during the early pathogenesis in patients with sleep-disordered breathing. © Springer Science+Business Media B.V. 2009.
DescriptionProceeding of the XVIIth ISAC Meeting (International Society for Arterial Chemoreception Meeting), School of Medicine of Valladolid, Valladolid, Spain, July 1–5, 2008
Persistent Identifierhttp://hdl.handle.net/10722/125338
ISSN
2015 Impact Factor: 1.953
2015 SCImago Journal Rankings: 0.887
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, SYen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorFung, MLen_HK
dc.date.accessioned2010-10-31T11:25:28Z-
dc.date.available2010-10-31T11:25:28Z-
dc.date.issued2009en_HK
dc.identifier.citationAdvances In Experimental Medicine And Biology, 2009, v. 648, p. 207-214en_HK
dc.identifier.issn0065-2598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125338-
dc.descriptionProceeding of the XVIIth ISAC Meeting (International Society for Arterial Chemoreception Meeting), School of Medicine of Valladolid, Valladolid, Spain, July 1–5, 2008-
dc.description.abstractThe carotid body (CB) plays important roles in cardiorespiratory changes in intermittent hypoxia (IH). Erythropoietin (EPO), a hypoxia-inducible factor (HIF)-1 target gene, is present in the chemoreceptive type-I cells in the CB but its expression and role in IH resembling sleep apnoeic conditions are not known. We hypothesized that IH upregulates the expression of EPO and its receptor (EPOr) in the rat CB. The CB expressions of EPO and EPOr were examined in rats breathing 10% O 2 (in isobaric chamber for CH, 24 hour/day) or in IH (cyclic between air and 5% O 2 per minute, 8 hour/day) for 3-28 days. Immunohistochemical studies revealed that the EPO and EPOr proteins were localized in CB glomic clusters. The proportional amount of cells with positive staining of EPO immunoreactivities was significantly increased in both IH and CH groups when compared with the normoxic control. The EPO expression was more markedly increased in the CH than that of the IH groups throughout the time course, reaching a peak level at day 14. The positive EPOr immunostaining was increased significantly in the 3-day CH group. By day 14, the EPOr expression elevated considerably at peak levels in both IH and CH rats, whereas the elevation was greater in the CH rats. These results suggest an upregulation of EPO and its receptor expression in the rat CB under IH and CH conditions, presumably mediated by the activation of HIF-1 pathway. The increased EPO binding to its receptor might play a role in the enhancement of CB excitability during the early pathogenesis in patients with sleep-disordered breathing. © Springer Science+Business Media B.V. 2009.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC.-
dc.relation.ispartofAdvances in Experimental Medicine and Biologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectCarotid bodyen_HK
dc.subjectChronic hypoxiaen_HK
dc.subjectErythropoietinen_HK
dc.subjectIntermittent hypoxiaen_HK
dc.subject.meshAnoxia - metabolism-
dc.subject.meshCarotid Body - drug effects - metabolism-
dc.subject.meshErythropoietin - metabolism-
dc.subject.meshReceptors, Erythropoietin - metabolism-
dc.subject.meshUp-Regulation - drug effects-
dc.titleUpregulation of erythropoietin and its receptor expression in the rat carotid body during chronic and intermittent hypoxiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0065-2598&volume=648&spage=207&epage=214&date=2009&atitle=Upregulation+of+erythropoietin+and+its+receptor+expression+in+the+rat+carotid+body+during+chronic+and+intermittent+hypoxiaen_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1007/978-90-481-2259-2_24en_HK
dc.identifier.pmid19536483-
dc.identifier.scopuseid_2-s2.0-67651211931en_HK
dc.identifier.hkuros173740en_HK
dc.identifier.hkuros160574-
dc.identifier.hkuros188471-
dc.identifier.hkuros188474-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67651211931&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume648en_HK
dc.identifier.spage207en_HK
dc.identifier.epage214en_HK
dc.identifier.isiWOS:000267288300024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLam, SY=7402279518en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK
dc.identifier.citeulike4923694-

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