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Article: Biomechanical Characterization of a Micro/Macroporous Polycaprolactone Tissue Integrating Vascular Graft

TitleBiomechanical Characterization of a Micro/Macroporous Polycaprolactone Tissue Integrating Vascular Graft
Authors
KeywordsBiomechanical properties
Fibroblast
Polycaprolactone
Porous-walled tubes
Scaffolds
Vascular grafts
X-ray microcomputed tomography μCT
Issue Date2010
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/biomed/journal/13239
Citation
Cardiovascular Engineering And Technology, 2010, v. 1 n. 3, p. 202-215 How to Cite?
AbstractThe objective of the present study was to characterize the short-term biomechanical properties of cast micro/macroporous poly(caprolactone) (PCL) tubes intended for application as tissue integrating blood vessel substitutes. Micro/macroporous PCL vascular grafts (5. 5 mm internal diameter, 7.5 mm external diameter) with defined macropore structures were produced by rapidly cooling PCL solutions containing dispersed gelatin particles in dry ice, followed by solvent and gelatin extraction. A Bose-Enduratec BioDynamic chamber configured for cardiovascular applications was used to measure the diametrical stability (dilation) of tubular samples under hydrodynamic flow conditions at 37 °C. Microporous PCL tubes withstood the hydrodynamic stresses induced by short, 2-min duration flow rates up to 1000 mL/min, which resulted in estimated internal pressures in excess of arterial pressure (80-130 mmHg). Micro/macroporous PCL tubes having a maximum macroporosity of 23% accommodated the hydrodynamic stresses generated by short duration, flow rates up to 1000 mL/min, which resulted in estimated internal pressures similar to venous pressure (30 mmHg). The dilation of microporous PCL tubes under short, (5 min) pulsatile flow conditions (1 Hz) increased from 10 to 100 μm with increasing mean flow rate from 50 to 500 mL/min. Both microporous and macroporous tubes exhibited a burst strength higher than 900 mmHg under hydrostatic fluid pressure, which is in excess of arterial pressure (80-130 mmHg) by a factor of approximately 7. Quantitative analysis of the macropore structure was performed using micro-computed tomography for correlation with mechanical properties and cell growth rates. Mouse fibroblasts efficiently colonized the external surface of macroporous PCL materials over 8 days in cell culture and cell numbers were higher by a factor of two compared with microporous PCL. These findings demonstrate that micro/macroporous PCL tubes designed for vascular tissue engineering can accommodate the hydrodynamic stresses generated by short duration, simulated blood flow conditions and exhibit good potential for integration with host tissue. © 2010 Biomedical Engineering Society.
Persistent Identifierhttp://hdl.handle.net/10722/125261
ISSN
2015 SCImago Journal Rankings: 0.415
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLam, Jen_HK
dc.contributor.authorZhang, Ben_HK
dc.contributor.authorTomlins, PEen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorAlpar, Oen_HK
dc.contributor.authorWertheim, DFen_HK
dc.contributor.authorJones, ASen_HK
dc.contributor.authorCoombes, AGAen_HK
dc.date.accessioned2010-10-31T11:20:39Z-
dc.date.available2010-10-31T11:20:39Z-
dc.date.issued2010en_HK
dc.identifier.citationCardiovascular Engineering And Technology, 2010, v. 1 n. 3, p. 202-215en_HK
dc.identifier.issn1869-408Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/125261-
dc.description.abstractThe objective of the present study was to characterize the short-term biomechanical properties of cast micro/macroporous poly(caprolactone) (PCL) tubes intended for application as tissue integrating blood vessel substitutes. Micro/macroporous PCL vascular grafts (5. 5 mm internal diameter, 7.5 mm external diameter) with defined macropore structures were produced by rapidly cooling PCL solutions containing dispersed gelatin particles in dry ice, followed by solvent and gelatin extraction. A Bose-Enduratec BioDynamic chamber configured for cardiovascular applications was used to measure the diametrical stability (dilation) of tubular samples under hydrodynamic flow conditions at 37 °C. Microporous PCL tubes withstood the hydrodynamic stresses induced by short, 2-min duration flow rates up to 1000 mL/min, which resulted in estimated internal pressures in excess of arterial pressure (80-130 mmHg). Micro/macroporous PCL tubes having a maximum macroporosity of 23% accommodated the hydrodynamic stresses generated by short duration, flow rates up to 1000 mL/min, which resulted in estimated internal pressures similar to venous pressure (30 mmHg). The dilation of microporous PCL tubes under short, (5 min) pulsatile flow conditions (1 Hz) increased from 10 to 100 μm with increasing mean flow rate from 50 to 500 mL/min. Both microporous and macroporous tubes exhibited a burst strength higher than 900 mmHg under hydrostatic fluid pressure, which is in excess of arterial pressure (80-130 mmHg) by a factor of approximately 7. Quantitative analysis of the macropore structure was performed using micro-computed tomography for correlation with mechanical properties and cell growth rates. Mouse fibroblasts efficiently colonized the external surface of macroporous PCL materials over 8 days in cell culture and cell numbers were higher by a factor of two compared with microporous PCL. These findings demonstrate that micro/macroporous PCL tubes designed for vascular tissue engineering can accommodate the hydrodynamic stresses generated by short duration, simulated blood flow conditions and exhibit good potential for integration with host tissue. © 2010 Biomedical Engineering Society.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/biomed/journal/13239en_HK
dc.relation.ispartofCardiovascular Engineering and Technologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectBiomechanical propertiesen_HK
dc.subjectFibroblasten_HK
dc.subjectPolycaprolactoneen_HK
dc.subjectPorous-walled tubesen_HK
dc.subjectScaffoldsen_HK
dc.subjectVascular graftsen_HK
dc.subjectX-ray microcomputed tomography μCTen_HK
dc.titleBiomechanical Characterization of a Micro/Macroporous Polycaprolactone Tissue Integrating Vascular Graften_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1869-408X&volume=1&issue=3&spage=202&epage=215&date=2010&atitle=Biomechanical+characterization+of+a+micro/macroporous+polycaprolactone+tissue+integrating+vascular+graft-
dc.identifier.emailLam, J: jkwlam@hku.hken_HK
dc.identifier.authorityLam, J=rp01346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s13239-010-0019-1en_HK
dc.identifier.scopuseid_2-s2.0-79953218699en_HK
dc.identifier.hkuros175319en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79953218699&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1en_HK
dc.identifier.issue3en_HK
dc.identifier.spage202en_HK
dc.identifier.epage215en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Y=23390880400en_HK
dc.identifier.scopusauthoridLam, J=8404243000en_HK
dc.identifier.scopusauthoridZhang, B=7406909365en_HK
dc.identifier.scopusauthoridTomlins, PE=6701753012en_HK
dc.identifier.scopusauthoridLi, X=35205953500en_HK
dc.identifier.scopusauthoridAlpar, O=24529734700en_HK
dc.identifier.scopusauthoridWertheim, DF=15137366100en_HK
dc.identifier.scopusauthoridJones, AS=7407103840en_HK
dc.identifier.scopusauthoridCoombes, AGA=7005603231en_HK

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