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Article: Multiple coding and non-coding RNAs in the Hoxb3 locus and their spatial expression patterns during mouse embryogenesis

TitleMultiple coding and non-coding RNAs in the Hoxb3 locus and their spatial expression patterns during mouse embryogenesis
Authors
KeywordsAnti-sense RNA
Expression patterns
Hoxb3
Initiation exon
Non-coding RNA
Promoter
Issue Date2010
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2010, v. 398 n. 2, p. 153-159 How to Cite?
AbstractHoxb3 plays important roles in embryogenesis and it has a complex transcription profile of mRNAs, non-coding RNAs and anti-sense RNAs. Characterization of the spatial expression patterns of these RNAs is important to understand their functions. We investigated the regulation and spatial expression patterns of multiple RNA transcripts derived from the Hoxb3 gene locus. By 5'-RACE we identified four novel transcription initiation sites and initiating exons, and by luciferase activity assay we identified a new promoter region. Expression pattern analysis of the alternative transcripts containing specific initiation exons in mouse embryos suggests that there are co-operations between the initiation exons, their adjacent promoters and enhancer elements to orchestrate overlapping neural tube specific transcription profiles for Hoxb3. Furthermore, we showed that anti-sense transcripts derived from the Hoxb3 locus were expressed in the hindbrain with distinct rhombomere boundaries, in a pattern complementary to the sense coding mRNA transcripts. Our results suggest that the multiple non-coding RNAs could be involved in the regulation of Hoxb3. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/124509
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

We thank Drs. K.W. Ling, Michelle Kahmeyer-Gabbe and Eric C.W. So for their experimental assistance. This work was supported by a CRCG Small Project Funding Grant from the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, KTen_HK
dc.contributor.authorQi, Jen_HK
dc.contributor.authorSham, MHen_HK
dc.date.accessioned2010-10-31T10:38:26Z-
dc.date.available2010-10-31T10:38:26Z-
dc.date.issued2010en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2010, v. 398 n. 2, p. 153-159en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/124509-
dc.description.abstractHoxb3 plays important roles in embryogenesis and it has a complex transcription profile of mRNAs, non-coding RNAs and anti-sense RNAs. Characterization of the spatial expression patterns of these RNAs is important to understand their functions. We investigated the regulation and spatial expression patterns of multiple RNA transcripts derived from the Hoxb3 gene locus. By 5'-RACE we identified four novel transcription initiation sites and initiating exons, and by luciferase activity assay we identified a new promoter region. Expression pattern analysis of the alternative transcripts containing specific initiation exons in mouse embryos suggests that there are co-operations between the initiation exons, their adjacent promoters and enhancer elements to orchestrate overlapping neural tube specific transcription profiles for Hoxb3. Furthermore, we showed that anti-sense transcripts derived from the Hoxb3 locus were expressed in the hindbrain with distinct rhombomere boundaries, in a pattern complementary to the sense coding mRNA transcripts. Our results suggest that the multiple non-coding RNAs could be involved in the regulation of Hoxb3. © 2010 Elsevier Inc.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, 2010, v. 398 n. 2, p. 153-159. DOI: 10.1016/j.bbrc.2010.05.150-
dc.subjectAnti-sense RNAen_HK
dc.subjectExpression patternsen_HK
dc.subjectHoxb3en_HK
dc.subjectInitiation exonen_HK
dc.subjectNon-coding RNAen_HK
dc.subjectPromoteren_HK
dc.subject.meshEmbryonic Development - genetics-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshHomeodomain Proteins - genetics-
dc.subject.meshRNA, Messenger - genetics-
dc.subject.meshRNA, Untranslated - genetics-
dc.titleMultiple coding and non-coding RNAs in the Hoxb3 locus and their spatial expression patterns during mouse embryogenesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=398&issue=2&spage=153&epage=159&date=2010&atitle=Multiple+coding+and+non-coding+RNAs+in+the+Hoxb3+locus+and+their+spatial+expression+patterns+during+mouse+embryogenesis-
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2010.05.150en_HK
dc.identifier.pmid20515659-
dc.identifier.scopuseid_2-s2.0-77955058078en_HK
dc.identifier.hkuros179582en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955058078&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume398en_HK
dc.identifier.issue2en_HK
dc.identifier.spage153en_HK
dc.identifier.epage159en_HK
dc.identifier.isiWOS:000280458400001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, KT=7406034062en_HK
dc.identifier.scopusauthoridQi, J=15623852600en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.citeulike7238941-

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