Interactions between Epstein Barr Viral (EBV) proteins and p53 tumor suppressor in the development of nasopharyngeal carcinoma (NPC)
Dr Cao, Liang (Principal investigator)
Emeritus Professor Ng Mun Hon (Co-Investigator)
Croucher Foundation - Research Grants
To confirm that functional p53 protein abnormality commonly seen in NPC tumors may be largely ascribed to epigenetic mechanisms, rather than aberrations of p53 gene; to identify the host and EBV specific proteins from these tumors that may influence the function of wild type p53 protein by binding directly or indirectly to it; to study the structure and function of such interacting protein complex by investigating: (a) nature of this association, i.e. whether the association is direct or indirect; (b) specificity of this interaction with wild type and mutant forms of p53 proteins; (c) transcription activation via p53 protein binding sites with wild type p53; (d) co-accumulation of both p53 and its viral (cellular) binding proteins inside individual cells; (e) conformational change of p53 protein upon the binding of the viral proteins; to analyze the natural occurrence of both p53 and the viral protein in NPC tumor specimens.