Development of DNA and viral vector vaccines against Epstein-Barr virus-positive nasopharyngeal carcinoma


Grant Data
Project Title
Development of DNA and viral vector vaccines against Epstein-Barr virus-positive nasopharyngeal carcinoma
Principal Investigator
Dr Lin, Chen-Lung   (Principal investigator)
Co-Investigator(s)
Professor Austyn Jonathan M.   (Co-Investigator)
Dr Xu Xiaoning   (Co-Investigator)
Duration
24
Start Date
2003-09-01
Completion Date
2005-08-31
Amount
624000
Conference Title
Presentation Title
Discipline
Cancer
Sponsor
RGC General Research Fund (GRF)
HKU Project Code
HKU 7444/03M
Grant Type
General Research Fund (GRF)
Funding Year
2003/2004
Status
Completed
Objectives
To design and parpare DNA vaccine encoding EBNA1 and LMP2, and the MVA vaccine encoding the cytokine GM-CSF, the chemokine RANTES, the EBV- specific lytic antigen EBNA-1, and EBV-specific latent antigen LMP2; to identify the efficacy of these vaccines in vitro on their capacity in attracting dendritic cells and also in the induction of EBNA-1 specific CD4+T cell response and LMP2-specific CTL immunity; to verify the efficacy of these vaccines in a mouse NPC and a B cell lymphoma model, including the potency of CD4+ and CTL immunity, and also to test the effect of the dosage, route, and the effect of combinational use of both the DNA and MVA vaccines.