Interactions between Epstein Barr Viral (EBV) proteins and p53 tumor suppressor in the development of nasopharyngeal carcinoma (NPC)
Dr Cao, Liang (Principal investigator)
Emeritus Professor Ng Mun Hon (Co-Investigator)
RGC General Research Fund (GRF)
HKU Project Code
General Research Fund (GRF)
To confirm that functional p53 protein abnormality commonly seen in NPC tumors may be largely ascribed to epigenetic mechanisms, rather than aberrations of p53 gene; to identify the host and EBV specific proteins from these tumors that may influence the function of wild type p53 protein by binding directly or indirectly to it; to study the structure and function of such interacting protein complex by investigating: (a) nature of this association, i.e. whether the association is direct or indirect; (b) specificity of this interaction with wild type and mutant forms of p53 proteins; (c) transcription activation via p53 protein binding sites with wild type p53; (d) co-accumulation of both p53 and its viral (cellular) binding proteins inside individual cells; (e) conformational change of p53 protein upon the binding of the viral proteins; to analyze the natural occurrence of both p53 and the viral protein in NPC tumor specimens.