High resolution 3D X-ray structural analysis and functional study of human glucose-6-phosphate dehydrogenase (G6PD): normal and mutant enzymes


Grant Data
Project Title
High resolution 3D X-ray structural analysis and functional study of human glucose-6-phosphate dehydrogenase (G6PD): normal and mutant enzymes
Principal Investigator
Dr Lam, Veronica Min Sien   (Principal investigator)
Co-Investigator(s)
Dr Adams Margaret J   (Co-Investigator)
Professor Engel Paul Cowper   (Co-Investigator)
Duration
53
Start Date
1998-11-01
Completion Date
2003-03-31
Amount
1197000
Conference Title
Presentation Title
Keywords
3D X-ray, glucose-6-phosphate dehydrogenase, mutant enzymes
Discipline
Biochemistry
Sponsor
RGC General Research Fund (GRF)
HKU Project Code
HKU 7272/98M
Grant Type
General Research Fund (GRF)
Funding Year
1998/1999
Status
Completed
Objectives
To understand the role of individual amino acid and how changes lead to enzyme deficiency, we need to obtain a higher resolution structure, at 2.5 A or better; to solve the wild type (B) enzyme; to obtain, a full set of kinetic data on substrate/coenzyme binding, stability of the proteins under varying conditins, susceptibility to thermal/chemical denaturation and ease of folding/renaturation in order to establish the primary functional basis of the disease, we can then interpret the data in the context of the structural details.