PIKE-A GTPase and Energy Metabolism


Grant Data
Project Title
PIKE-A GTPase and Energy Metabolism
Principal Investigator
Dr Chan, Chi Bun   (Principal investigator)
Duration
36
Start Date
2016-09-01
Completion Date
2019-08-31
Amount
1424600
Conference Title
Presentation Title
Keywords
AMPK, Lipid, Obesity, PIKE-A
Discipline
Diabetes/Metabolism,Signal Transduction
Panel
Biology and Medicine (M)
Sponsor
RGC Early Career Scheme (ECS)
HKU Project Code
27100816
Grant Type
Early Career Scheme (ECS)
Funding Year
2016/2017
Status
On-going
Objectives
2. To identify the metabolic factors that regulate PIKE-A/AMPK association. PIKE-A interacts with AMPK in obese muscles, yet the hormonal signals or intracellular events that govern the protein complex formation remain unexplored. To address this issue, we will examine the effect of TNFα on PIKE-A/AMPK interaction in C2C12 cells. We will also determine if the presence of PIKE-A is critical for TNFα-provoked AMPK inhibition in cultured myotubes. These data will provide a new mechanistic insight on obesity-induced PIKE-A/AMPK association. 3. To study the impacts of PIKE-A/AMPK dissociation on energy metabolism. We will use muscle-specific PIKE knockout (MPKO) mice as the in vivo model to assess the role PIKE-A/AMPK complex in systemic glucose and energy metabolisms. Physiological parameters including growth rate, body composition, blood biochemistry, insulin sensitivity, lipid oxidation and energy expenditure profiles of high-fat-diet-fed Flox/Flox and MPKO mice will be studied.