PIKE-A GTPase and Energy Metabolism
Dr Chan, Chi Bun (Principal investigator)
AMPK, Lipid, Obesity, PIKE-A
Biology and Medicine (M)
RGC Early Career Scheme (ECS)
HKU Project Code
Early Career Scheme (ECS)
2. To identify the metabolic factors that regulate PIKE-A/AMPK association. PIKE-A interacts with AMPK in obese muscles, yet the hormonal signals or intracellular events that govern the protein complex formation remain unexplored. To address this issue, we will examine the effect of TNFα on PIKE-A/AMPK interaction in C2C12 cells. We will also determine if the presence of PIKE-A is critical for TNFα-provoked AMPK inhibition in cultured myotubes. These data will provide a new mechanistic insight on obesity-induced PIKE-A/AMPK association. 3. To study the impacts of PIKE-A/AMPK dissociation on energy metabolism. We will use muscle-specific PIKE knockout (MPKO) mice as the in vivo model to assess the role PIKE-A/AMPK complex in systemic glucose and energy metabolisms. Physiological parameters including growth rate, body composition, blood biochemistry, insulin sensitivity, lipid oxidation and energy expenditure profiles of high-fat-diet-fed Flox/Flox and MPKO mice will be studied.