Molecular Mechanism of PACT-dependent Innate Antiviral Response


Grant Data
Project Title
Molecular Mechanism of PACT-dependent Innate Antiviral Response
Principal Investigator
Professor Jin, Dong-Yan   (Project coordinator)
Co-Investigator(s)
Dr Yeung Man Lung   (Co-principal investigator)
Professor Zheng Bojian   (Co-principal investigator)
Dr Zhou Jie   (Co-principal investigator)
Professor Yuen Kwok Yung   (Co-principal investigator)
Professor Au Shannon Wong Ngor   (Co-principal investigator)
Professor Sham Mai Har   (Co-principal investigator)
Dr Chan Chi Ping   (Co-principal investigator)
Duration
36
Start Date
2016-06-01
Completion Date
2019-05-31
Amount
7555731
Conference Title
Presentation Title
Keywords
virus-host interaction, innate antiviral response, PACT, influenza A virus, hepatitis B virus
Discipline
Virology,Infection/Parasitology
Panel
Medicine
Sponsor
RGC Collaborative Research Fund (CRF)
HKU Project Code
C7011-15R
Grant Type
Collaborative Research Fund (CRF) - Group Research Project
Funding Year
2015/2016
Status
On-going
Objectives
2. We will determine the dual antiviral function of PACT in IAV infection. The interaction of PACT with viral polymerase subunits PA, PB1 and PB2 as well as their mutual antagonism will be characterized. We will elucidate the mechanism by which PA, PB1 and PB2 inhibit PACT activation of innate antiviral response. We will also shed light on the roles of PACT in viral RNA sensing and in the inhibition of viral replication. 3. We will determine the dual antiviral function of PACT in the context of HBV infection. The requirement of PACT for innate sensing of the ε element on pgRNA of HBV and the induction of type III IFNs will be established. The molecular determinants on the ε element required for the activation of PACT will be delineated. The mechanism by which PACT inhibits the function of the ?? element in HBV replication will be elucidated.