Natural Products Molecular Targets Identification by Quantitative Pathway Analysis


Grant Data
Project Title
Natural Products Molecular Targets Identification by Quantitative Pathway Analysis
Principal Investigator
Dr Fung, Eva Yi Man   (Principal investigator)
Co-Investigator(s)
Dr Lok Chun Nam   (Co-Investigator)
Duration
36
Start Date
2015-09-01
Completion Date
2018-08-31
Amount
782205
Conference Title
Presentation Title
Keywords
HPLC/MS-MS, Traditional Chinese Medicine, Proteomics, Bioinformatics, Molecular target
Discipline
Proteomics
Panel
Biology and Medicine (M)
Sponsor
RGC General Research Fund (GRF)
HKU Project Code
17127915
Grant Type
General Research Fund (GRF)
Funding Year
2015/2016
Status
On-going
Objectives
2 Aim 2. To apply the optimized procedure to search for novel molecular targets of selected bioactive TCM components. The same procedure has been applied to another bioactive component in herbs, corydaline, an alkaloid found in corydalis rhizome. Our preliminary study with corydaline hypothesized the proto-oncogene c-fos as one of the potential targets. Other than corydaline, a few bioactive TCM components will be selected based on their cytotoxcities, and the target identification procedure will be applied subsequently. Several TCM has been targeted based on their pharmacological effects stated in traditional Chinese medicinal literature. The selected TCM including: Dendrobium nobile (Shihu), Juncus effusus (Dengxincao), Lithospermum erythrorhizon (Zicao), Houttuynia cordata (Yuxingcao) and dandelion (Pugongying), their major bioactive components will be used in the proposed research. 3 Aim 3. To validate the potential targets of the selected bioactive TCM components and to elucidate their mechanism-of-action. A potential targets generation method is helpful in providing research direction to study the mechanism-of-action of a drug. The proposed potential molecular targets found in Aim 2 will be validated through orthogonal biological assays. And the mechanism-of-action of the bioactive TCM components will be elucidated through the identified targets. We are validating the proto-oncogene c-fos as a target of corydaline. Our preliminary results showed that corydaline inhibits a c-fos related transcriptional activity.