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Title of Dataset
Data from: A Structurally and Functionally Biomimetic Biphasic Scaffold for Intervertebral Disc Tissue Engineering
Author of Dataset
Choy, Andrew Tsz Hang1
Chan, Barbara Pui1
Date of Dataset Creation
Tissue engineering offers high hopes for the treatment of intervertebral disc (IVD) degeneration. Whereas scaffolds of the disc nucleus and annulus have been extensively studied, a truly biomimetic and mechanically functional biphasic scaffold using naturally occurring extracellular matrix is yet to be developed. Here, a biphasic scaffold was fabricated with collagen and glycosaminoglycans (GAGs), two of the most abundant extracellular matrix components in the IVD. Following fabrication, the scaffold was characterized and benchmarked against native disc. The biphasic scaffold was composed of a collagen-GAG co-precipitate making up the nucleus pulposus-like core, and this was encapsulated in multiple lamellae of photochemically crosslinked collagen membranes comprising the annulus fibrosus-like lamellae. On mechanical testing, the height of our engineered disc recovered by ~82-89% in an annulus-independent manner, when compared with the 99% recovery exhibited by native disc. The annulus-independent nature of disc height recovery suggests that the fluid replacement function of the engineered nucleus pulposus core might mimic this hitherto unique feature of native disc. Biphasic scaffolds comprised of 10 annulus fibrosus-like lamellae had the best overall mechanical performance among the various designs owing to their similarity to native disc in most aspects, including elastic compliance during creep and recovery, and viscous compliance during recovery. However, the dynamic mechanical performance (including dynamic stiffness and damping factor) of all the biphasic scaffolds was similar to that of the native discs. This study contributes to the rationalized design and development of a biomimetic and mechanically viable biphasic scaffold for IVD tissue engineering.
Choy, ATH, Chan, BP. (2015). Data from: A Structurally and Functionally Biomimetic Biphasic Scaffold for Intervertebral Disc Tissue Engineering. [Data File]. All relevant data are within the paper and its Supporting Information files. Click on “Linked Publications” to access the publication and access supporting information on figshare at
Subject (RGC Codes)
M2 — Medicine, Dentistry & Health — 醫學, 牙科學及保健
  • 1241 — Biomedical Engineering — 生物醫學工程
Subject (ANZSRC)
  • 0903 — BIOMEDICAL ENGINEERING — 生物醫學工程
    • 090301 — Biomaterials — 生物材料
Biomimetic Biphasic Scaffold
biphasic scaffold
extracellular matrix components
Intervertebral Disc Tissue Engineering Tissue engineering
nucleus pulposus core
photochemically crosslinked collagen membranes
fluid replacement function
disc height recovery
IVD tissue engineering
  1. Univ Hong Kong, Dept Mech Engn, Tissue Engn Lab, Hong Kong, Hong Kong, Peoples R China