Dataset

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Title of Dataset
Data from: Sign epistasis limits evolutionary trade-offs at the confluence of single- and multi-carbon metabolism in Methylobacterium extorquens AM1
Author of Dataset
Carroll, Sean Michael1
Lee, Ming-Chun2
Marx, Christopher James3
Contact
Marx, Christopher James3
Date of Dataset Creation
2013-10-08
Description
Adaptation of one set of traits is often accompanied by attenuation of traits important in other selective environments, leading to fitness trade-offs. The mechanisms that either promote or prevent the emergence of trade-offs remain largely unknown, and are difficult to discern in most systems. Here, we investigate the basis of trade-offs that emerged during experimental evolution of Methylobacterium extorquens AM1 to distinct growth substrates. After 1500 generations of adaptation to a multi-carbon substrate, succinate (S), many lineages had lost the ability to use one-carbon compounds such as methanol (M), generating a mixture of M+ and M− evolved phenotypes. We show that trade-offs in M− strains consistently arise via antagonistic pleiotropy through recurrent selection for loss-of-function mutations to ftfL (formate-tetrahydrofolate ligase), which improved growth on S while simultaneously eliminating growth on M. But if loss of FtfL was beneficial, why were M trade-offs not found in all populations? We discovered that eliminating FtfL was not universally beneficial on S, as it was neutral or even deleterious in certain evolved lineages that remained M+. This suggests that sign epistasis with earlier arising mutations prevented the emergence of mutations that drove trade-offs through antagonistic pleiotropy, limiting the evolution of metabolic specialists in some populations.
Citation
Carroll, SM, Lee, MC, Marx, CJ. (2013). Data from: Sign epistasis limits evolutionary trade-offs at the confluence of single- and multi-carbon metabolism in Methylobacterium extorquens AM1. [Data File]. Access to Files at http://dx.doi.org/10.5061/dryad.n2645
Subject (RGC Codes)
M1 — Biological Sciences — 生物科學
  • 1102 — Biochemistry — 生物化學
Subject (ANZSRC)
11 — MEDICAL AND HEALTH SCIENCES — 醫學與衛生科學
  • 1101 — MEDICAL BIOCHEMISTRY AND METABOLOMICS — 醫學生物化學和代謝
    • 110101 — Medical Biochemistry: Amino Acids and Metabolites — 醫學生物化學
Keyword
Adaptation
Epistasis
Trade-Offs
Physiology
Pleiotropy
Selection - Experimental
Affiliations
  1. Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA
  2. Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA ; Univ Hong Kong, Dept Biochem, Pok Fu Lam, Hong Kong, Peoples R China
  3. Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA ; Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA